New cryptic karyotypic differences between cattle (Bos taurus) and goat (Capra hircus)

Lorenzi, L. De; Planas, Jordi; Rossi, Elena; Malagutti, Luca; Parma, P.

doi:10.1007/s10577-014-9462-4

Cited by 13 publications

(12 citation statements)

References 18 publications

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“…2). The present finding is in agreement with the earlier reports [4] in indigenous cattle, and the karyotype investigation is being used for evolution of species as well as for comparison among species [5][6][7]. The cytogenetic studies were also used for screening the chiameric calves [8] and ruling out fertility problems in female [9].…”

Section: Resultssupporting

confidence: 90%

Complete Cytogenetic Insight of Tho-Tho Cattle

Longkumer¹,

Mukherjee²,

Yenisetti³

et al. 2015

JAST-A

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Abstract:To investigate the cytogenetic parameters and characterise the chromosomal banding pattern of Tho-Tho cattle, a breed of indigenous cattle found in the Northeastern states of India were reared for meat purpose. Lymphocyte culture technique was carried out in 28 Tho-Tho cattle and culture was harvested for good metaphase spread. Good metaphase spreads were selected for analysis, such as relative length, centromeric index and arm ratio. Centromeric banding (C-banding) and reverse banding (R-banding) methods were done for detail and better understanding of the chromosome morphology. The chromosome number in Tho-Tho cattle was observed to be 2n = 60 in all complete metaphase. The mean relative length of the autosomal chromosomes varied from 5.48% ± 0.107% to 1.79% ± 0.105% in male and 5.31% ± 0.148% to 1.86% ± 0.055% in female, respectively. The chromosome banding showed C-positive dark band heterochromatin in all the acrocentric autosome. However, in sex chromosome, the Y-chromosome showed negative C-band and also the X-chromosome did not show any stain at the centromeric region. The numbers of R-band pattern were observed to be 490 and 499 band in male and female, respectively. One of the X-chromosome showed light banding pattern, confirming the inactivation during the embryonic development in female. The fundamental chromosome number and banding pattern of Tho-Tho cattle did not vary from the other breed of the Bos indicus. However, it is necessary to start a cytogenetic screening of the Tho-Tho cattle and expand upon more number to be kept at different villages of Nagaland in order to identify animals with chromosomal abnormalities, so that it can be excluded from future breeding strategies for conservation of Tho-Tho genetic resource.

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Section: Resultssupporting

confidence: 90%

Complete Cytogenetic Insight of Tho-Tho Cattle

Longkumer¹,

Mukherjee²,

Yenisetti³

et al. 2015

JAST-A

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“…An alternative to the high resolution BAC mapping is the in silico bioinformatic approach, which allows to detect cryptic chromosomal divergencies. However, this strategy is limited only to the species with sequenced and well assembled genomes [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

Karyotype relationships among selected deer species and cattle revealed by bovine FISH probes

et al. 2017

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The Cervidae family comprises more than fifty species divided into three subfamilies: Capreolinae, Cervinae and Hydropotinae. A characteristic attribute for the species included in this family is the great karyotype diversity, with the chromosomal numbers ranging from 2n = 6 observed in female Muntiacus muntjak vaginalis to 2n = 70 found in Mazama gouazoubira as a result of numerous Robertsonian and tandem fusions. This work reports chromosomal homologies between cattle (Bos taurus, 2n = 60) and nine cervid species using a combination of whole chromosome and region-specific paints and BAC clones derived from cattle. We show that despite the great diversity of karyotypes in the studied species, the number of conserved chromosomal segments detected by 29 cattle whole chromosome painting probes was 35 for all Cervidae samples. The detailed analysis of the X chromosomes revealed two different morphological types within Cervidae. The first one, present in the Capreolinae is a sub/metacentric X with the structure more similar to the bovine X. The second type found in Cervini and Muntiacini is an acrocentric X which shows rearrangements in the proximal part that have not yet been identified within Ruminantia. Moreover, we characterised four repetitive sequences organized in heterochromatic blocks on sex chromosomes of the reindeer (Rangifer tarandus). We show that these repeats gave no hybridization signals to the chromosomes of the closely related moose (Alces alces) and are therefore specific to the reindeer.

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“…Once we identified the chromosome with the neocentromere (using BAC 200C01, not shown), we proceeded with the selection of several specific BACs of BTA17 as previously reported [De Lorenzi et al, 2015]. All BACs tested were on the "new" BTA17p arm ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Centromere Repositioning in Cattle (<b><i>Bos taurus</i></b>) Chromosome 17

Lorenzi

Iannuzzi²,

Rossi³

et al. 2017

Cytogenet Genome Res

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Eukaryotic organisms have developed a structure, called centromere, able to preserve the integrity of the genome during cell division. A young bull from the Marchigiana breed, with a normal external phenotype, underwent routine cytogenetic analysis to enter the reproduction center. All metaphases analyzed showed an unusual biarmed chromosome of medium size despite a diploid set of chromosomes (2n = 60,XY). FISH analysis excluded a pericentric inversion or a reciprocal translocation, but highlighted a repositioning of the centromere in BTA17. The satellite DNA was still in an acrocentric position. The telomeres were normally present. The primary constriction on the abnormal chromosome was C-band negative. Finally, the absence of a large genomic deletion in the BTA17 pericentromeric region was demonstrated by both array-CGH analysis and SNP array. To our knowledge, this is the first case of centromere repositioning reported in cattle.

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New cryptic karyotypic differences between cattle (Bos taurus) and goat (Capra hircus)

Cited by 13 publications

References 18 publications

Complete Cytogenetic Insight of Tho-Tho Cattle

Complete Cytogenetic Insight of Tho-Tho Cattle

Karyotype relationships among selected deer species and cattle revealed by bovine FISH probes

Centromere Repositioning in Cattle (<b><i>Bos taurus</i></b>) Chromosome 17

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