New Cytotoxic Norditerpene Dilactones from Leaves of Podocarpus macrophyllus var. maki.

Park, Hyun‐Sun; Kai, Noriyuki; Fukaya, Haruhiko; Aoyagi, Yutaka; Takeya, Koichi

doi:10.1002/chin.200422183

Cited by 2 publications

(2 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some of these compounds have antimicrobial, fungistatic, or bacteriostatic properties [33], [37], [38]. Other compounds have cytotoxic properties that may be useful in destroying cancer [39]–[43].…”

Section: Introductionmentioning

confidence: 99%

DNA Barcode Identification of Podocarpaceae—The Second Largest Conifer Family

2013

View full text Add to dashboard Cite

We have generated matK, rbcL, and nrITS2 DNA barcodes for 320 specimens representing all 18 extant genera of the conifer family Podocarpaceae. The sample includes 145 of the 198 recognized species. Comparative analyses of sequence quality and species discrimination were conducted on the 159 individuals from which all three markers were recovered (representing 15 genera and 97 species). The vast majority of sequences were of high quality (B 30 = 0.596–0.989). Even the lowest quality sequences exceeded the minimum requirements of the BARCODE data standard. In the few instances that low quality sequences were generated, the responsible mechanism could not be discerned. There were no statistically significant differences in the discriminatory power of markers or marker combinations (p = 0.05). The discriminatory power of the barcode markers individually and in combination is low (56.7% of species at maximum). In some instances, species discrimination failed in spite of ostensibly useful variation being present (genotypes were shared among species), but in many cases there was simply an absence of sequence variation. Barcode gaps (maximum intraspecific p–distance > minimum interspecific p–distance) were observed in 50.5% of species when all three markers were considered simultaneously. The presence of a barcode gap was not predictive of discrimination success (p = 0.02) and there was no statistically significant difference in the frequency of barcode gaps among markers (p = 0.05). In addition, there was no correlation between number of individuals sampled per species and the presence of a barcode gap (p = 0.27).

show abstract

Section: Introductionmentioning

confidence: 99%

DNA Barcode Identification of Podocarpaceae—The Second Largest Conifer Family

2013

View full text Add to dashboard Cite

show abstract

“…Some of these compounds have antimicrobial, fungistatic, or bacteriostatic properties (7,11,12]. Other compounds have cytotoxic properties that may be useful in destroying cancer (13,14,15,16,17].…”

Section: Introductionmentioning

confidence: 99%

Molecular Identification of Podocarpus spp. from Bukidnon and Batanes, Philippines using rbcL sequences

2016

IJACEBS

View full text Add to dashboard Cite

Abstract-Podocarpus(Podocarpaceae) consists of approximately 100 species spreading in the tropical and subtropical regions worldwide and also in temperate regions in the Southern Hemisphere. It is often difficult to accurately identify Podocarpus. DNA barcoding technique is helpful to provide rapid and accurate taxonomic identification using a specific DNA region. In this study, unknown Podocarpus samples are identified using rbcL sequences. Leaf samples were collected from Bukidnon and Batanes, Philippines. DNA extraction, PCR amplification and DNA sequencing were consequently conducted. The rbcL sequence of Podocarpus is about 700 bp in length. Molecular identification using Basic Local Alignment Search Tool (BLAST) for nucleotides reveals that the sample from Bukidnon is Podocarpus macrophyllus while that from Batanes is Podocarpus costalis. This identification may be helpful as an addition to the reference library of Podocarpaceae. This may allow researchers unfamiliar with the family's morphology and anatomy make accurate identification.

show abstract

New Cytotoxic Norditerpene Dilactones from Leaves of Podocarpus macrophyllus var. maki.

Cited by 2 publications

References 1 publication

DNA Barcode Identification of Podocarpaceae—The Second Largest Conifer Family

DNA Barcode Identification of Podocarpaceae—The Second Largest Conifer Family

Molecular Identification of Podocarpus spp. from Bukidnon and Batanes, Philippines using rbcL sequences

Contact Info

Product

Resources

About