New Data on Taxonomic and Geographic Distribution of the trnLUAA Intron Deletion of Chloroplast DNA in Hedysarum L. (Fabaceae L.)

Нуждина, Н. С.; Bondar, Alexander A.; Дорогина, О. В.

doi:10.1134/s1022795418110108

Cited by 4 publications

(5 citation statements)

References 44 publications

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“…The nuclear ETS marker allowed us to clarify relationships between two sections, Hedysarum and Multicaulia with 20 sampled accessions. Results of DNA sequencing presented herein demonstrated that the relationship registered between two sections using ETS sequences was consistent with the trnL-F chloroplast data [6].…”

Section: Resultssupporting

confidence: 78%

“…Our results revealed incongruence between ETS-tree location and subsectional affinities among Multicaulia section taxa. Species belonging to Subacaulia and Multicaulia subsections [4] were dispersed chaotically which has evidenced the unnatural subdivision of Multicaulia section, whereas the sequences of trnL-F locus of cpDNA analyzed in our previous study [6] have discovered the heterogeneity (biphyly) of section Hedysarum. Taking into consideration the intermediate position of H. ferganense at ETS phylogenetic tree, we can assume therefore that our data supports the hypothesis of non-monophyletic origin of Hedysarum proposed by Liu et al (2017).…”

Section: Resultsmentioning

confidence: 77%

“…setigerum, and its widespread congener H. gmelinii. Hence, the molecular DNA similarity between H. gmelinii and its stemless subspecies evidenced from trnL-F sequence data previously [6] has been improved. Another two local endemics, H. chaiyrakanicum and H. dahuricum possessed a high molecular similarity according their ETS nucleotide sequences (88 BS).…”

Section: Resultsmentioning

confidence: 98%

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DNA specificity in two Hedysarum sections, Hedysarum and Multicaulia (Siberia, Russia) inferred from ETS sequence data

2021

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Based on sequence data of the nuclear ETS markers of 20 species, the relationships of sections Hedysarum and Multicaulia were assessed. Phylogenetic analysis was performed on the complete sequences using the Neighbor-Joining methods. The nuclear marker ETS is investigated for the first time in the most species excluding H. hedysaroides, H. inundatum, H. ferganense, and H. neglectum. The length of sequences varies from 349 to 357 nucleotides. It is noteworthy that ETS data significantly discriminate H. ferganense from both sections, Hedysarum and Multicaulia. Moreover, our results did not support the subsectional division of Multicaulia section. Therefore, the ETS sequence data obtained in our study for 20 taxa of Hedysarum evidence the nonmonophyly of section Multicaulia.

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Section: Resultssupporting

confidence: 78%

Section: Resultsmentioning

confidence: 77%

Section: Resultsmentioning

confidence: 98%

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DNA specificity in two Hedysarum sections, Hedysarum and Multicaulia (Siberia, Russia) inferred from ETS sequence data

2021

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“…The species-speci c chromosomal markers found in the karyotypes of H. alpinum, H. caucasicum and H. neglectum may be useful in taxonomic studies of this section (Zviagina, et al 2013, Yurkevich, et al 2021. According to the literature, the phylogenetic difference between the two species H. theinum and H. neglectum was 59 percent (Nuzhdina, et al 2018).…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, an extended molecular phylogenetic study of the tribe Hedysareae has been proposed using cDNA marking. To study the phylogeny of the genus and to differentiate species, recent nucleotide sequence studies of nuclei and chloroplast origins have con rmed the polyphyletic nature of the genus Hedysarum (Nuzhdina et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of Anatomical, Phytochemical and Molecular Genetic Characteristics of Two Species of Hedysaruml.

Kusmangazinov

Kurmanbayeva

Zharkova

et al. 2022

Preprint

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Background Hedysarum L. is one of the large families of the Fabaceae, consisting of 200 species. Species in the genus Hedysarum are valuable forage and medicinal resources. In this paper we studied Hedysarum theinum Krasnob. and Hedysarum neglectum Ledeb. growing in the Western Altai of Eastern Kazakhstan. H.theinum is a perennial, herbaceous, valuable medicinal plant, a rare high-altitude species, an Altai-Dzungarian endemic and globally endangered species. H. neglectum is a perennial plant of high medicinal and fodder value, growing mainly in the sub-alpine zone. A complex phytochemical study of Hedysarum using modern methods, expanding the data on herbal medicines is actual. At the current time, taxonomic and phylogenetic questions of the genus Hedysarum are the subject of close attention, as the systematic knowledge of the genus Hedysarum L. Fabaceae: Hedysareae is still incomplete. Therefore, questions are being actively addressed by correlating data obtained by molecular genetic analyses. In the study, route-recognition methods were used in a field search of two species of Hedysarum for comparative morphological study in 2020–2021. Results The morphological measurements showed a difference in the external structure of the two species. Structural changes in the anatomical structure of the leaf, stem and root were analyzed as the plants adapted to different conditions in the Western Altai. The changes were expressed in the transition from the typical dorsiventral leaf structure of H.theinum to the isolateral-palisade structure of the mesophyll of H.neglectum. The phytochemical analysis revealed the highest amount of ethyl α-d glucopyranoside at 45.23% in the subterranean part of H. theinum, which was only 0.8% in H. neglectum. The root part of H. theinum contained the highest vitamin E content of 11.36%, while the above-ground part of H. neglectum showed an insignificant amount of tocopherol at 0.37%. Also the above-ground and underground parts of the two species contain squalene, which has an antioxidant effect and strengthens the immune system. Nowadays, an urgent task in pharmacognosy is the use of all kinds of DNA analysis methods for the identification of plant raw materials. In this regard, the possibility of using molecular genetic methods of research in the analysis of representatives of the genus Hedysarum L., for the identification of medicinal plant raw materials was studied. Conclusion Phylogenetic analysis using molecular genetic markers revealed that H. neglectum and H. theinum belong to the Hedysarum section. The data obtained can be used for the species identification of H.theinum and H.neglectum, which has so far been difficult due to the lack of some systematic morphological characters. The results obtained may be useful for the taxonomic studies of this section.

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Molecular characterization of Dittrichia viscosa (L.) greuter (Asteraceae) populations revealed by ISSR markers and chloroplast (CPDNA) tRNL intron sequences

Sevindik,

Bozkurt,

Yilmaz

et al. 2023

Genetika

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In this study, molecular characterization of Dittrichia viscosa (L.) Greuter populations collected from Ayd?n province was carried out based on ISSR technique and chloroplast DNA trnL intron sequences. 10 ISSR primers were used to determine the molecular characterization among the populations. For cpDNA trnL intron amplification, trnC and trnD primers were used. In ISSR analysis, a total of 70 bands were obtained. The polymorphism rate was determined to be approximately 94.28%. According to the ISSR analysis, the UPGMA dendrogram consisted of three groups. For cpDNA trnL intron sequences, phylogenetic analyses were obtained along with genetic distances. For populations, cpDNA trnL intron sequences were determined between 454 and 472 bases. The maximum likelihood phylogenetic tree consist of two clades. In addition, cpDNA trnL intron sequences of some species (Chrysophthalmum Pulicaria Inula Jasonia Stenachaenium Carpesium Blumea Iphiona Limbarda Rhanterium Lifago Duhaldea Chiliadenus) of Asteraceae family from NCBI with Dittrichia viscosa populations phylogenetic tree was constructed. As a result of the study, it was determined that the polymorphism rate of Dittrichia viscosa populations obtained by ISSR-PCR was higher than the results obtained from trnL intron sequences.

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New Data on Taxonomic and Geographic Distribution of the trnLUAA Intron Deletion of Chloroplast DNA in Hedysarum L. (Fabaceae L.)

Cited by 4 publications

References 44 publications

DNA specificity in two Hedysarum sections, Hedysarum and Multicaulia (Siberia, Russia) inferred from ETS sequence data

DNA specificity in two Hedysarum sections, Hedysarum and Multicaulia (Siberia, Russia) inferred from ETS sequence data

Comparative Analysis of Anatomical, Phytochemical and Molecular Genetic Characteristics of Two Species of Hedysaruml.

Molecular characterization of Dittrichia viscosa (L.) greuter (Asteraceae) populations revealed by ISSR markers and chloroplast (CPDNA) tRNL intron sequences

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