New data on the level of immune stratum against Q fever agent in population of the of Republic of Guinea

Найденова, Е. В.; Каливоги, С; Карташов, М. Ю.; Бойко, А В; Бумбали, С.; Сафронов, Валентин; Захаров, К. С.; Nasser, Abdelkader; Dramé, Fatou Maria; Konstantinov, O. K.; Магасуба, Н.; Буаро, М. Й.; Щербакова, Светлана; Кутырев, Владимир

doi:10.15789/2220-7619-ndo-1485

Cited by 2 publications

(2 citation statements)

References 7 publications

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“…Coxiella burnetii is a gram-negative bacterium that resides within cells and is responsible for causing Q fever [ 39 ]. This infectious disease can be transmitted to humans through the consumption of contaminated products or the inhalation of aerosols containing the bacteria [ 40 ].…”

Section: Discussionmentioning

confidence: 99%

“…Our findings align with previous studies that have reported the presence of C. burnetii in ticks in Africa [ 47 ] and contribute to our understanding of its circulation in RG. Previously, epidemiological data on this bacterium in RG had been limited to the detection of bacterium-specific antibodies in humans and animals [ 39 , 48 ]. Furthermore, we confirm earlier observations that C. burnetii can be found in ticks collected from small mammals [ 7 , 49 ].…”

Section: Discussionmentioning

confidence: 99%

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Epidemiology of Zoonotic Coxiella burnetii in The Republic of Guinea

Ohlopkova,

Yakovlev,

Emmanuel

et al. 2023

Microorganisms

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Background: Q fever is a zoonotic infectious disease characterized by fever, malaise, chills, significant weakness, and muscle pain. In some cases, the disease can become chronic and affect the inner membranes of the heart, such as the valves, leading to endocarditis and a high risk of death. Coxiella burnetii (C. burnetii) is the primary causative agent of Q fever in humans. This study aims to monitor the presence of C. burnetii in ticks collected from small mammals and cattle in the Republic of Guinea (RG). Methods: Rodents were trapped in the Kindia region of RG during 2019–2020, and ticks were collected from cattle in six regions of RG. Total DNA was extracted using a commercial kit (RIBO-prep, InterLabService, Russia) following the manufacturer’s instructions. Real-time PCR amplification was conducted using the kit (AmpliSens Coxiella burnetii-FL, InterLabService, Russia) to detect C. burnetii DNA. Results and Conclusions: Bacterial DNA was detected in 11 out of 750 (1.4%) small mammals and 695 out of 9620 (7.2%) tick samples. The high number of infected ticks (7.2%) suggests that they are the main transmitters of C. burnetii in RG. The DNA was detected in the liver and spleen of a Guinea multimammate mouse, Mastomys erythroleucus. These findings demonstrate that C. burnetii is zoonotic in RG, and measures should be taken to monitor the bacteria’s dynamics and tick prevalence in the rodent population.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Epidemiology of Zoonotic Coxiella burnetii in The Republic of Guinea

Ohlopkova,

Yakovlev,

Emmanuel

et al. 2023

Microorganisms

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Current data on the circulation of the Q fever pathogen in the Republic of Guinea

Naidenova,

Zakharov,

Agafonov

et al. 2024

Journal of microbiology, epidemiology and immunobiology

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Background. Q fever is the one of the best-studied zoonoses, which is widespread throughout almost the entire territory of Africa, excluding the territory of the Sahara. However, the current data on the incidence of coxiellosis and the circulation of Coxiella burnetii on this continent are limited and vary according different sources. In 1980–1990, the Soviet-Guinean Research Virology and Microbiology Laboratory conducted studies to estimate the distribution of the Q fever pathogen, assess the herd immunity in humans and identify specific antibodies in the sera of livestock. However, in subsequent years, the research was suspended. The aim of this study is to obtain up-to-date data on the distribution of C. burnetii in all landscape and geographical zones of the Republic of Guinea. Materials and methods. The study was carried out in the laboratory of the Russian-Guinean Center for Epidemiology and Prevention of Infectious Diseases (Kindia, Republic of Guinea). The study involved 332 sera of febrile patients and 3156 sera from practically healthy volunteers, 1074 blood samples of livestock, 1648 suspensions of ticks, 319 specimens of small mammals and 298 of bats. The study was carried out using ELISA and PCR methods, selected samples were subjected to in-depth genetic analysis. Results and discussion. The study of the distribution of C. burnetii on the territory of all landscape-geographical zones of the Republic of Guinea was carried out. For the first time, an officially registered case of human Q fever case has been identified. The role of livestock, small mammals and bats in the circulation of the pathogen has been established. It has been shown that the main vectors in Guinea are ixodid ticks of the Amblyomma variegatum, Hyalomma truncatum and Rhipicephalus decoloratus species. Employing molecular methods, C. burnetii strains carrying the QpH1 plasmid, capable of causing diseases in humans and animals were identified. For the first time, the complete nucleotide sequence of 16S rRNA of the Q fever pathogen (OQ152497–OQ152500) identified on the territory of Guinea was determined and registered in the GenBank database. Conclusion. Taking into account the high epidemiological significance of Q fever, the study of the specifics of C. burnetii circulation in Guinea remains an urgent task. Regular monitoring and assessment of risk factors for diseases caused by coxiella are necessary for the development of an algorithm for laboratory diagnosis and recommendations for clinicians.

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New data on the level of immune stratum against Q fever agent in population of the of Republic of Guinea

Cited by 2 publications

References 7 publications

Epidemiology of Zoonotic Coxiella burnetii in The Republic of Guinea

Epidemiology of Zoonotic Coxiella burnetii in The Republic of Guinea

Current data on the circulation of the Q fever pathogen in the Republic of Guinea

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