New detection of Cyprinid herpesvirus 2 associated with mass mortality in colour crucian carp ( Carassius auratus ), in China

Abstract

During October 2016, a mass mortality of colour crucian carp (Carassius auratus), which the affected fish were lethargic, inappetence and anoxic, was occurred in a fish farm located in Chengdu, Sichuan province, China. To elucidate the aetiology of this outbreak, histological and electron microscope examination, molecular investigation were conducted. Pathologic examination revealed multi foci necrosis on haematopoietic organs, gills, hearts and pancreas. Transmission electron microscopy observations exhibited sphere herpesvirus‐like particles distributed amongst the tissues of gill, spleen and kidney. Molecular analysis is verified that the causative agent of this outbreak was Cyprinid herpesvirus 2 (CyHV‐2). This report first report CyHV‐2 in colour crucian carp, which increases the concern about damage of CyHV‐2 and its potential role in species.

Cyprinid herpesvirus‐2 (CyHV‐2): a comprehensive review

“…2016; Zhao et al . 2019), posing threat for food security also, especially in China where more than 2.5 M metric tons of crucian carp and Prussian carp are produced (FAO 2014). Thus, considering the significance of fish trade worldwide, and emerging data on CyHV‐2 infections from various countries, improvements in its diagnosis and prophylaxis are called for to limit its occurrence and impacts to aquaculture.…”

“…However, scientific data on the disease caused by Cyprinid herpesvirus2 (CyHV-2), the aetiological agent of a highly contagious viral disease, namely herpesviral haematopoietic necrosis disease (HVHND), are very scattered in spite of massive damage caused by the disease to production of goldfish (C. auratus) and many other Cyprinids (Panicz et al 2019). Besides goldfish, recently, the disease has been reported from other species of the same genus like C. gibelio (Prussian carp) (Doszpoly et al 2011;Xu et al 2013) and C. carassius (crucian carp) (Fichi et al 2016;Zhao et al 2019), posing threat for food security also, especially in China where more than 2.5 M metric tons of crucian carp and Prussian carp are produced (FAO 2014). Thus, considering the significance of fish trade worldwide, and emerging data on CyHV-2 infections from various countries, improvements in its diagnosis and prophylaxis are called for to limit its occurrence and impacts to aquaculture.…”