New Developments in Profiling and Imaging of Proteins from Tissue Sections by MALDI Mass Spectrometry

Chaurand, Pierre; Norris, Jeremy L.; Cornett, Dale S.; Mobley, James A.; Caprioli, Richard M.

doi:10.1021/pr060346u

Cited by 262 publications

(265 citation statements)

References 59 publications

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“…M atrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) is a powerful technique for analyzing the spatial distribution of a wide range of biomolecules [1][2][3]. Phospholipids are integral structural and regulatory components of biological membranes, important in cell signaling pathways, and they serve as precursors to many active biomolecules [4 -7].…”

mentioning

confidence: 99%

Solvent-free matrix dry-coating for MALDI imaging of phospholipids

Puolitaival

Burnum

Cornett

et al. 2008

J. Am. Soc. Mass Spectrom.

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214

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A fast and simple, solvent-free matrix deposition protocol was developed for positive ionization mode phospholipid analysis in tissues. Finely ground 2,5-dihydroxybenzoic acid was deposited onto sagittal mouse brain sections using a dry-coating technique, in which solid matrix particles were filtered directly onto the tissue through a 20-m stainless steel sieve. Phospholipid signals were obtained directly off these sections, allowing acquisition of high-resolution MS images. These images were compared to those from serial sections that were spray-coated with a thin-layer chromatography (TLC) reagent sprayer. Signals obtained from the dry matrix deposition method were comparable to those from spray-coated sections, producing identical localization patterns with a simpler and faster sample preparation with virtually no analyte delocalization. This approach was found to yield highly reproducible results, eliminating much of the variance caused by operator differences, and making it an attractive alternative to the currently used matrix application methods. (J

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mentioning

confidence: 99%

Solvent-free matrix dry-coating for MALDI imaging of phospholipids

Puolitaival

Burnum

Cornett

et al. 2008

J. Am. Soc. Mass Spectrom.

Self Cite

214

208

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“…The basis of MALDI was developed by Koichi Tanaka (Nobel laureate in chemistry in 2002), and sophisticated by Franz Hillenkamp and Michael Karas (Karas and Hillenkamp 1988), allowing the analysis of large numbers of biomolecules ranging from small metabolite molecules (m/z<1000) (Garrett et al 2006;Khatib-Shahidi et al 2006) to much larger proteins with molecular weights of 10 5 (Chaurand et al 2006;Stoeckli et al 2002), by covering them with a matrix and ionizing them using a pulse laser beam. High-molecular weight materials, such as proteins and DNA, are fragile and tend to be fragmented when ionized by conventional ionization methods.…”

Section: Principle Of Msmentioning

confidence: 99%

Imaging mass spectrometry: principle and application

2009

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Imaging mass spectrometry (IMS) is twodimensional mass spectrometry to visualize the spatial distribution of biomolecules, which does not need either separation or purification of target molecules, and enables us to monitor not only the identification of unknown molecules but also the localization of numerous molecules simultaneously. Among the ionization techniques, matrix assisted laser desorption/ionization (MALDI) is one of the most generally used for IMS, which allows the analysis of numerous biomolecules ranging over wide molecular weights. Proper selection and preparation of matrix is essential for successful imaging using IMS. Tandem mass spectrometry, which is referred to MS n , enables the structural analysis of a molecule detected by the first step of IMS. Applications of IMS were initially developed for studying proteins or peptides. At present, however, targets of IMS research have expanded to the imaging of small endogenous metabolites such as lipids, exogenous drug pharmacokinetics, exploring new disease markers, and other new scientific fields. We hope that this new technology will open a new era for biophysics.

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“…However, in many cases, the most interesting signals are those observed between 2000 and 30 000 m/z, which include low-mw signaling molecules (chemokines, cytokines, growth factors, etc.) [54]. MS-Imaging technique has been used in several pathologies [55][56][57][58], although the application to vascular tissues has not been described yet.…”

Section: Ms-imaging Of Atheroma Plaquesmentioning

confidence: 99%

Vascular proteomics

Vivanco

Mas

Dardé

et al. 2007

Proteomics Clinical Apps

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The characterization of patients with acute coronary syndromes (ACS) at the molecular and cellular levels provides a novel vision for understanding the pathological and clinical expression of the disease. Recent advances in proteomic technologies permit the evaluation of systematic changes in protein expression in many biological systems and have been extensively applied to cardiovascular diseases (CVD). The cardiovascular system is in permanent intimate contact with blood, making blood-based biomarker discovery a particularly worthwhile approach. Thus, proteomics can potentially yield novel biomarkers reflecting CVD, establish earlier detection strategies, and monitor response to therapy. Here we review the different proteomic strategies used in the study of atherosclerosis and the novel proteins differentially expressed and secreted by atherosclerotic lesions which constitute novel potential biomarkers (HSP-27, Cathepsin D). Special attention is paid to MS-Imaging of atheroma plaque and the generation, for the first time, of 2-D images of lipids, showing the distribution of these molecules in the different areas of the atherosclerotic lesions. In addition new potential biomarkers have been identified in plasma (amyloid A1a, transtherytin), circulating cells (protein profile in monocytes from ACS patients) and individual cells constituents of atheroma plaques (endothelial, VSMC, macrophages) which provide novel insights into vascular pathophysiology.

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New Developments in Profiling and Imaging of Proteins from Tissue Sections by MALDI Mass Spectrometry

Cited by 262 publications

References 59 publications

Solvent-free matrix dry-coating for MALDI imaging of phospholipids

Solvent-free matrix dry-coating for MALDI imaging of phospholipids

Imaging mass spectrometry: principle and application

Vascular proteomics

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