New developments in the molecular epidemiology of adenovirus 8 keratoeonjunctivitis

Jong, J.C. de; Demazure, M.; Legrand-Quillien, Marie-Christine; Lay, G. Le; Colin, Joseph; Wermenbol, A. G.; Verweij-Uÿterwaal, Marja W.; Avoort, H. G. A. M. Van Der; Chastel, C

doi:10.1002/jmv.1890380206

Cited by 17 publications

(16 citation statements)

References 21 publications

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“…Adrian et al (1990) used six RE patterns in alphabetical order (BamHI, BglII, HindIII, KpnI, SalI and SmaI). Later, BglI, BstEII and SacI were included in successive studies (de Jong et al, 1992;Tanaka et al, 2000). The cleavage pattern of the prototypes for all REs was labelled as enzyme code 1.…”

Section: Genome Type Designation Based On Numerical Order: European Cmentioning

confidence: 99%

“…HAdV-8/D1 is, by definition, the DNA variant to which prototype strain Trim belongs. So far, HAdV-8/D1-HAdV-8/D12 have been described (Table 2) (Adrian et al, 1990;de Jong et al, 1992;Tanaka et al, 2000). In this classification system, BglI and HindIII are the most discriminating enzymes, followed by BamHI, BglII and SalI.…”

Section: Genome Type Designation Based On Numerical Order: European Cmentioning

confidence: 99%

“…This method enables prediction of a possible correlation between genomic variation and virulence of the strain. So far, HAdV-8A-8K and HAdV-8/ D1-D12 have been described using the Asian and European classification systems of genome typing, respectively (Fujii et al, 1983Fujii & Yamashita, 1984;Ishii et al, 1987;Sawada et al, 1987;Sheu et al, 1987;Guo et al, 1988;Chang et al, 2001;Adhikary et al, 2003Adhikary et al, , 2011Ohguchi et al, 2003;Jin et al, 2011;Takács et al, 1983;Adrian et al, 1990;de Jong et al, 1992;Tanaka et al, 2000). Nevertheless, there have also been several problems with genome typing, such as the application of variable numbers and types of REs as well as difficulties in the interpretation of results due to the lack of a published catalogue of HAdV-8 genome types.…”

Section: Introductionmentioning

confidence: 99%

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Human adenovirus type 8 genome typing

Adhikary

Ushijima

Fujimoto

2012

Journal of Medical Microbiology

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Section: Genome Type Designation Based On Numerical Order: European Cmentioning

confidence: 99%

Section: Genome Type Designation Based On Numerical Order: European Cmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Human adenovirus type 8 genome typing

Adhikary

Ushijima

Fujimoto

2012

Journal of Medical Microbiology

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“…The HAdV-8 clinical strains were HAdV-8b and HAdV-8e, and belonged to different genotypes (Adrian et al, 1990;De Jong et al, 1992). The clinical strains of HAdV-19 and HAdV-37 were HAdV-19a, HAdV-19/1997and HAdV-19/2001, and HAdV-37/1991, HAdV-37/1996and HAdV-37/2004, respectively.…”

mentioning

confidence: 99%

Analysis of the complete genome sequence of epidemic keratoconjunctivitis-related human adenovirus type 8, 19, 37 and a novel serotype

Kaneko

Iida

Ishiko

et al. 2009

Journal of General Virology

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We determined the complete genome sequence of epidemic keratoconjunctivitis (EKC)-related human adenoviruses (HAdVs). We analysed a total of 12 HAdV strains; three prototype strains and two HAdV-8, three HAdV-19 and three HAdV-37 clinical isolates from EKC patients in Japan, and one novel serotype of HAdV. Genome organization of these serotypes was identical to those of the recently determined HAdV-19 and HAdV-37. The identities of the whole genome were over 99 % among strains from the same serotype, except for HAdV-19p, which is not associated with conjunctivitis, resulting in the formation of a distinct cluster in the phylogenetic analysis. The penton, loop 1 and loop 2 of hexon, early region 3 (E3) and fiber were hypervariable regions between serotypes. Results suggest that the HAdV-19 clinical strain is a recombinant of HAdV-19p-like and HAdV-37-like strains, and that the acquisition of the penton, E3 or fiber may be related to ocular tropism.Adenoviruses are nonenveloped, double-stranded DNA viruses with icosahedral capsids (Swenson et al., 2003). Human adenoviruses (HAdVs) belong to the genus Mastadenovirus of the family Adenoviridae and are classified into six species, A to F (HAdV-A to HAdV-F) (Benkö et al., 2000;Wold & Horwitz, 2007). Adenoviral conjunctivitis is mainly caused by HAdV-3 (in HAdV-B), HAdV-4 (in HAdV-E), and HAdV-8, , with the three HAdV-D serotypes being known to cause epidemic keratoconjunctivitis (EKC). HAdV-8 is the original causative agent of EKC and remains the predominant HAdV serotype isolated in association with EKC in many countries (Ishii et al., 1987;Chang et al., 2001;Vainio et al., 2001;Aoki & Tagawa, 2002; Jin et al., 2006). In Japan, although HAdV-8 and HAdV-19 have been described, a novel serotype of HAdV recently isolated from EKC patients and HAdV-37 are the predominant causative serotype of EKC in Japan (Higuchi et al., 1987;Aoki & Tagawa, 2002;Ishiko et al., 2008;Kaneko et al., 2008).Nucleotide polymorphisms in HAdV strains isolated from EKC patients can be classified into discrete genotypes within a specific HAdV serotype on the basis of their restriction endonuclease cleavage pattern (Wadell et al., 1980;Adrian et al., 1986). It has been speculated that the appearance of new genotypes might contribute to the incidence of outbreaks of each serotype (Aoki & Tagawa, 2002;Ariga et al., 2005). DNA sequence analysis has allowed us to appreciate the molecular evolution of HAdV in greater detail, and revealed that the penton, hexon and fiber genes were the most variable among the different serotypes (Pring-Akerblom & Adrian, 1995;Arnberg et al., 1997;Ebner et al., 2005;Madisch et al., 2005Madisch et al., , 2007 MiuraOchiai et al., 2007). The complete genome sequences of 24 HAdV serotypes 2, 3, 4, 5, 7, 9, 11, 12, 14, 16, 17, 19, 21, 26, 34, 35, 37, 40, 41, 46, 48, 49 and 50) have now been determined.In this study, we describe the complete genome sequences of the prototype strains HAdV-8p, HAdV-19p and HAdV37p together with a novel HAdV serotype and eight clinical

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“…2 We have found that closely related genomic variants of adenovirus 8 were in circulation in the Strasbourg population during nine years. Unfortunately, we had only limited information about the patients, so it is diYcult to establish the aYliation between these identical strains.…”

Section: Discussionmentioning

confidence: 88%

Molecular epidemiology of ocular isolates of adenovirus 8 obtained over nine years.

et al. 1999

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Twenty nine strains of adenovirus 8 have been isolated over nine years in Strasbourg, France, 22 of which were from one private ophthalmologist. To assess a possible relation between these strains, the DNA of adenovirus was analysed by restriction fragment length polymorphism using eight diVerent enzymes. Among these, three proved discriminant (Xba I, Bgl II, Eco RI) and made it possible to define 13 genotypes diVering from each other by one to three DNA bands. Seven genotypes were unique isolates, while three, representing 16 strains, were isolated over five to eight years. All the genotypes but one were closely related, with 87% homology. All 13 diVered from an adenovirus 8 strain from Lyon (homology 68-76%). This study confirmed the stability of adenovirus 8 in a given population. (J Clin Pathol 1999;52:860-861) Keywords: adenovirus 8; molecular epidemiology; eye isolates Adenovirus 8 is a common cause of epidemic keratoconjunctivitis that may be transmitted by ocular examination apparatus. These infections are without any long term consequences on eye function. The epidemiology of adenoviruses is currently under investigation using restriction fragment length polymorphism (RFLP) of the entire adenovirus genome. This allows identification of genomic variants (genotypes) and analysis of their distribution in time and space. 1-5Methods From 1989 to 1997, 29 strains of adenovirus 8 have been isolated in the virology laboratory, Faculty of Medicine, Strasbourg, France. Twenty two strains were from one private ophthalmologist, three from another private specialist, and four from diVerent units of the Strasbourg University Hospital.All the strains were isolated on human diploid fibroblasts, MRC-5, serotyped by seroneutralisation or immunofluorescence with a monoclonal antibody at the time of isolation, and stored at −80°C. For RFLP analysis, viruses were grown on MRC-5 cells. The intracellular viral DNA was extracted according to the method of Shinagawa et al 6 modified by Li et al. 7 Briefly, infected cells were recovered in a 50 ml tube, pelleted (1500 g, 15 minutes, 4°C), and suspended in 1.5 ml of TE buVer (10 mM Tris-HCl, 1 mM EDTA, pH 7.9). Cells were lysed by 0.01% sodium dodecyl sulphate (SDS) in 1 M NaCl overnight at 4°C. The cellular debris was pelleted (17 500 g, 30 minutes, 4°C) and the virus containing supernatant was extracted with an equal volume of phenol. The phenolic phase was extracted twice with equal volumes of TE buVer, after which 1.5 volumes of 95% ethanol were added to the aqueous phase and left overnight at −20°C. After centrifugation (1500 g, 15 minutes, 4°C) the pellet was suspended in 500 µl of lysis buVer (0.5% SDS, 100 mM NaCl, 50 µg/ml proteinase K) and incubated at 37°C for about one hour. After two successive extractions with equal volumes of phenol and ether, the aqueous phase was incubated with an equal volume of isopropanol overnight at −20°C. After centrifugation (17 000 g, 20 minutes, 4°C), the pellet of purified adenovirus DNA was dissolved in 100 µl of TE buV...

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New developments in the molecular epidemiology of adenovirus 8 keratoeonjunctivitis

Cited by 17 publications

References 21 publications

Human adenovirus type 8 genome typing

Human adenovirus type 8 genome typing

Analysis of the complete genome sequence of epidemic keratoconjunctivitis-related human adenovirus type 8, 19, 37 and a novel serotype

Molecular epidemiology of ocular isolates of adenovirus 8 obtained over nine years.

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