New developments in two photon endoscopy

Schwarz, Martin K.; Riemann, Iris; Weinigel, Martin; König, Karsten; Messerschmidt, Bernhard; Harzic, Ronan Le

doi:10.1117/12.808781

Cited by 3 publications

(3 citation statements)

References 21 publications

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“…The two-photon rigid microendoscope module in combination with the DermaInspect JL DI 017 10 was employed in wound healing studies in human subjects after permission by the Ethics Committee. Also a flexible nonlinear optical microendoscope was developed at JenLab based on a compact two axes piezo scanner and a miniature objective lens (51)(52)(53)(54) but not used in vivo.…”

Section: Two-photon Microendoscopementioning

confidence: 99%

Review: history of multiphoton tomography

König

2023

Multiphoton Microscopy in the Biomedical Sciences XXIII

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Two decades ago, the very first prototype of a multiphoton tomograph was built and delivered as a high-resolution skin imaging device to the cosmetic company Beiersdorf AG on November 2, 2001. The tomograph was based on a tunable 80 MHz NIR femtosecond laser system and able to detect two-photon excited autofluorescence intensity and fluorescence lifetime based on time-correlated single photon counting (TCSPC) as well as second harmonic generation (SHG) with 300 nm lateral resolution and 250 ps temporal resolution. Two-photon fluorescence lifetime imaging (FLIM) was performed based in a biexponential decay and false-color coding. The first clinical in vivo study on melanocytic lesions with the DermaInspect started at the Department of Dermatology of the University Jena on September 5, 2003. The study was performed on 53 patients and resulted in optical biopsies based on 2108 optical sections. The tomograph worked under clinical conditions without any problem. Finally, the CE0118 certificate for the multiphoton tomograph DermaInspect as medical device was granted by the European Certified Body Thuringian State Authority of Metrology and Verification (LMET) on December 22, 2004. The DermaInspect became the world’s first femtosecond laser medical device for clinical imaging. The CE0118 certificate for the 2nd generation of multiphoton tomographs, the portable medical device with a tunable titanium:sapphire laser, an optical arm and a 360° measurement head mounted on a mechanical arm, the “MPTflex”, was granted on Nov 8, 2010. The third generation, the multimodal multiphoton tomograph MPTcompact, is based on a chiller-free ultracompact femtosecond fiber laser and miniaturized detectors to measure autofluorescence, SHG, FLIM, and confocal reflectance. The first two MPTcompact prototypes were delivered to hospitals in Heidelberg and Gera in 2018 to realize a multicenter clinical study on the detection of malignant melanoma. JenLab’s multiphoton tomographs have been used to realize the word’s first clinical in vivo two-photon imaging of patients, including (i) the first two-photon fluorescence lifetime imaging, (ii) the first microendoscopy, the first (iii) diseased skin imaging, (iv) brain imaging, and (v) the first CARS studies.

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Section: Two-photon Microendoscopementioning

confidence: 99%

Review: history of multiphoton tomography

König

2023

Multiphoton Microscopy in the Biomedical Sciences XXIII

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“…The main advantages of the GRIN lenses over conventional lenses are the small diameters down to 0.2 mm (maximum 2 mm) and the flat surfaces that allow easy assembly of complex optical systems. A more recent report on the development of a miniaturized two-photon fluorescence endoscope using GRIN lenses demonstrated that this technique is highly flexible and controllable in terms of time acquisition, resolution, and magnification and yet capable of discerning and resolving microscopic cellular details [75].…”

Section: Two-photon Induced Photoluminescence (Tpip) Imagingmentioning

confidence: 99%

Theranostic Applications of Gold Nanoparticles in Cancer

Diagaradjane

Mohindra

Krishnan

2011

Nanoplatform‐Based Molecular Imaging

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“…Along with other nonlinear imaging techniques, this technology has shown great potential for clinical diagnostic, leading to a strong interest for flexible fiber-based nonlinear endoscopy. Recently, several demonstrations were accomplished for two-photon fluorecence (TPEF) [2] and second harmonic generation (SHG) [3]. To date, many technical challenges hinder the development of a CARS endoscope.…”

Section: Introductionmentioning

confidence: 99%

Design and modeling of a prototype fiber scanning CARS endoscope

et al. 2010

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An endoscope capable of Coherent Anti-Stokes Raman scattering (CARS) imaging would be of significant clinical value for improving early detection of endoluminal cancers. However, developing this technology is challenging for many reasons. First, nonlinear imaging techniques such as CARS are single point measurements thus requiring fast scanning in a small footprint if video rate is to be achieved. Moreover, the intrinsic nonlinearity of this modality imposes several technical constraints and limitations, mainly related to pulse and beam distortions that occur within the optical fiber and the focusing objective.Here, we describe the design and report modeling results of a new CARS endoscope. The miniature microscope objective design and its anticipated performance are presented, along with its compatibility with a new spiral scanningfiber imaging technology developed at the University of Washington. This technology has ideal attributes for clinical use, with its small footprint, adjustable field-of-view and high spatial-resolution. This compact hybrid fiber-based endoscopic CARS imaging design is anticipated to have a wide clinical applicability.

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New developments in two photon endoscopy

Cited by 3 publications

References 21 publications

Review: history of multiphoton tomography

Review: history of multiphoton tomography

Theranostic Applications of Gold Nanoparticles in Cancer

Design and modeling of a prototype fiber scanning CARS endoscope

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