New Diagnostic Possibilities for Neonatal Sepsis

Tzialla, Chryssoula; Manzoni, Paolo; Achille, Cristian; Bollani, Lina; Stronati, Mauro; Borghesi, A.

doi:10.1055/s-0038-1639361

Cited by 37 publications

(40 citation statements)

References 15 publications

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“…Some inflammatory markers and cytokines have been investigated for the determination of an ongoing infection. However, the incidence at which each marker begins to show increasing and the time to normalization differs through each marker [6–8].…”

Section: Introductionmentioning

confidence: 99%

Presepsin and fetuin-A dyad for the diagnosis of proven sepsis in preterm neonates

et al. 2019

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Background Diagnosis is the most strenuous step in the evaluation of neonatal sepsis. No gold standard diagnostic method is available except for blood culture. We aimed to investigate the role of positive and negative acute phase reactants, namely presepsin and fetuin-A, in the diagnosis of culture-proven late-onset sepsis. Methods A prospective, case-control study with the infants ≤32 weeks of age with a diagnosis of culture-proven late-onset sepsis was designed. Twenty-nine preterm infants with similar gestational and postnatal ages without sepsis constituted the control group. Serum values of presepsin, fetuin-A, C-reactive protein and interleukin-6 were evaluated at the enrollment, third and seventh days of the diagnosis in the infants with positive blood culture results. Results First-day presepsin values were significantly higher in the culture-positive infants than the control group [1583 ng/L (1023–1731) vs. 426 ng/L (287–589), p = < 0.0001]. Presepsin was found to have an 88.9% sensitivity and 88.9% specificity with a cut-off value of 823 ng/ml for culture-proven LOS in our study, and area under the receiver-operating curve was 0.939. Fetuin-A levels were similar between the study and control groups ( p > 0.05). Conclusion Presepsin may be an accurate marker for both diagnosis and monitoring of treatment response for culture-proven late-onset sepsis in preterm infants. However, fetuin-A does not seem to be a useful tool for the diagnosis of sepsis.

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Section: Introductionmentioning

confidence: 99%

Presepsin and fetuin-A dyad for the diagnosis of proven sepsis in preterm neonates

et al. 2019

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“…Neonatal sepsis (NS) is a leading cause of morbidity and mortality in newborns (3), and it may be categorized as early-onset NS (EOS) or late-onset NS (LOS) (4). The morbidity and mortality of NS have been reduced due to progress made in neonatal care (5). However, there have been minimal advances in the specific clinical management and accuracy of diagnostic testing options over the last several decades (6).…”

Section: Introductionmentioning

confidence: 99%

Downregulation of miR‑1184 serves as a diagnostic biomarker in neonatal sepsis and regulates LPS‑induced inflammatory response by inhibiting IL‑16 in monocytes

Wang

Han

2021

Exp Ther Med

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Neonatal sepsis (NS) remains a global problem. In the present study, abnormal expression of microRNA-1184 (miR-1184) was detected in neonates with NS and it was endeavored to investigate the diagnostic value of miR-1184, as well as its regulatory role in lipopolysaccharide (LPS)-induced inflammatory response in vitro. Furthermore, the correlation between interleukin-16 (IL-16) and miR-1184 was investigated to elucidate the pathological mechanisms of NS development. Reverse transcription-quantitative PCR was used to detect the expression of miR-1184. Receiver operating characteristic curve analysis was performed to evaluate the diagnostic value of miR-1184 in NS. Furthermore, a sepsis cell model was established by using LPS-induced monocytes to explore the effect of miR-1184 on the inflammatory response. The levels of inflammatory cytokines were determined by ELISA. A luciferase reporter assay was used to investigate the direct targeting interaction between miR-1184 and IL-16. The results indicated that the serum levels of miR-1184 in neonates with sepsis were decreased and miR-1184 had a high diagnostic value when differentiating NS from respiratory conditions in neonates. In vitro, the expression of miR-1184 in monocytes was inhibited by LPS and overexpression of miR-1184 reversed the effect of LPS to stimulate the inflammatory response. IL-16 was demonstrated to be a target of miR-1184 and a negative correlation between them was identified in patients with NS. The inflammatory response inhibited by miR-1184 mimics was enhanced by overexpression of IL-16 in LPS-induced monocytes. In conclusion, decreased levels of serum miR-1184 may be a potential diagnostic biomarker for NS. In addition, miR-1184 inhibited the LPS-induced inflammatory response by targeting IL-16 in monocytes, suggesting that the miR-1184/IL-16 axis may be a potential therapeutic target for NS.

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“…Neonatal sepsis is an in ammatory system syndrome caused by bacteria or viruses, which is the main cause of neonatal morbidity and death [1][2][3].Researchers have been looking for an ideal biomarker with high sensitivity and speci city in order to diagnose and eliminate neonatal sepsis as early as possible [4].Currently, the most commonly used biomarkers are C-reactive protein(CRP),micro-erythrocyte sedimentation rate and procalcitonin (PCT) and serum amyloid A [5][6][7].However, the speci city of these indicators is poor, and their clinical application is limited.So far, no ideal biomarker can meet the standard [8,9].Therefore, exploring new diagnostic methods and molecular mechanisms is essential for neonatal sepsis.…”

Section: Introductionmentioning

confidence: 99%

MIR-141 is Negatively Correlated With TLR4 in Neonatal Sepsis and Regulates LPS-induced Inflammatory Responses in Monocytes

Lin

Wang

2020

Preprint

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Background: Neonatal sepsis is an inflammatory system syndrome caused by bacteria or viruses, which is the main cause of neonatal morbidity and mortality. But currently there is a lack of an ideal biomarker to meet the standard. The abnormally expression of miRNAs may be a group of potential diagnostic biomarkers in neonatal sepsis.Methods: First, qRT-PCR was used to calculate the expression of miR-141 in the serum of neonatal with sepsis and control. The diagnostic value of PCT and serum miR-141 was evaluated by receiver operating characteristic (ROC) curve. Second, the relationship between miR-141 and TLR4 was verified using luciferase report assay. An inflammation model was established using monocytes with the LPS treatment. Elisa analyzed the relationship between miR-141 and inflammatory factors.Results: qRT-PCR found that the expression of miR-141 in neonatal sepsis was significantly lower than healthy controls. ROC curve shows that miR-141 has high diagnostic accuracy. LPS stimulation in monocytes also led to a decrease in the expression of miR-141. Luciferase report assay proves that miR-141 can target and regulate TLR4 expression. ELISA proved that overexpression of miR-141 has the effect of inhibiting the LPS-induced inflammation in monocytes.Conclusion: In conclusion, miR-141 serves as a candidate diagnostic biomarker and is involved in the regulation of inflammatory response in the development of neonatal sepsis. Overexpression of miR-141 inhibits LPS-induced inflammation by targeting TLR4. Therefore, miR-141 is expected to be a potential diagnostic biomarker and a therapeutic target in neonatal sepsis.

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New Diagnostic Possibilities for Neonatal Sepsis

Cited by 37 publications

References 15 publications

Presepsin and fetuin-A dyad for the diagnosis of proven sepsis in preterm neonates

Presepsin and fetuin-A dyad for the diagnosis of proven sepsis in preterm neonates

Downregulation of miR‑1184 serves as a diagnostic biomarker in neonatal sepsis and regulates LPS‑induced inflammatory response by inhibiting IL‑16 in monocytes

MIR-141 is Negatively Correlated With TLR4 in Neonatal Sepsis and Regulates LPS-induced Inflammatory Responses in Monocytes

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