Abstract. In this study, the optimization of the surface plasmon resonance (SPR) sensor based on graphene-silver substrate was investigated. We simulated the refl ection spectrum that depends on the metal thickness and the number of graphene layers. The addition of a certain number of graphene layers based on the knowledge that silver oxidation decreases the sensitivity of the sensor improved the sensitivity S RI , while the detection accuracy decreased. To optimize the sensor performance, the investigation focused on monolayer graphene. Furthermore, genetic algorithms were used to optimize the SPR biosensor refl ection by adjusting the coeffi cients of the system, which are the incidence angle and metal layer thickness.