Recently, there has been a surge in interest in evaluating research accomplishments based on publications using bibliometric tools. This study aims to investigate the top 100 most cited articles in Neem using bibliometric analysis while also providing readers with a practical guide for determining the most influential publications over the past decades. VOSviewer 1.6.6, the "Bibliometrix app" in R. studio cloud, and SPSS were used to analyze the data. The top 100 highly-cited articles in Neem, which included 91 articles and nine reviews, were published between 1971 and 2017. The articles received a total citation score of 14,186 (citations ranging from 65 to 1,531), with an average total citation score of 141.9 and a total citation score per article of 10.07. 398 authors from 22 countries contributed a total of 100 publications, with Bhattacharyya KG being the most productive and influential. Researchers from India published about 45 percent of the articles and had the highest number of citations (8637). The Banaras Hindu University in India was discovered to be the top-ranked institution, with six publications and 1,491 citations. The top 100 literature was published in 72 journals, with the Journal of Ethnopharmacology publishing nine papers. The findings of this study provided extensive information and research direction for future research in this domain, including the most influential journals, countries, authors, institutions, and articles and mapping co-authorship and reported keywords.