New effects of GABAB receptor allosteric modulator rac-BHFF on ambient GABA, uptake/release, Em and synaptic vesicle acidification in nerve terminals

Pozdnyakova, Natalia; Dudarenko, Marina; Borisova, Тatiana

doi:10.1016/j.neuroscience.2015.07.037

Cited by 13 publications

(5 citation statements)

References 40 publications

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“…The synaptosomes were loaded with [ 3 H]GABA (50 nM, 4.7 µCi/ml) in the standard saline solution for 10 min. [ 3 H]GABA radioactivity was measured in aliquots of supernatants by liquid scintillation counting with Sigma-Fluor® High Performance LSC Cocktail according to (Pozdnyakova et al 2015 ).…”

Section: Methodsmentioning

confidence: 99%

A comparative study of wood sawdust and plastic smoke particulate matter with a focus on spectroscopic, fluorescent, oxidative, and neuroactive properties

Tarasenko

Pozdnyakova

Paliienko

et al. 2022

Environ Sci Pollut Res

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Here, water-suspended smoke aerosol preparation was synthesized from biomass-based fuel, i.e., a widespread product for residential heating, wood sawdust (WP) (pine, poplar, and birch mixture), and its properties were compared in parallel experiments with the smoke preparation from plastics (PP). Molecular groups in the PM preparations were analyzed using Raman and Fourier-transform infrared spectroscopy. WP was assessed in neurotoxicity studies using rat cortex nerve terminals (synaptosomes). Generation of spontaneous and H 2 O 2 -evoked reactive oxygen species (ROS) detected using fluorescent dye 2′,7′-dichlorofluorescein in nerve terminals was decreased by WP. In comparison with PP, WP demonstrated more pronounced reduction of spontaneous and H 2 O 2 -evoked ROS production. WP completely inhibited glutamate receptor agonist kainate-induced ROS production, thereby affecting the glutamate receptor-mediated signaling pathways. WP decreased the synaptosomal membrane potential in fluorimetric experiments and the synaptosomal transporter-mediated uptake of excitatory and inhibitory neurotransmitters, L-[ 14 C]glutamate and [ 3 H] γ-aminobutyric acid (GABA), respectively. PP decreased the ambient synaptosomal level of [ 3 H]GABA, whereas it did not change that of L-[ 14 C]glutamate. Principal difference between WP and PP was found in their ability to influence the ambient synaptosomal level of [ 3 H]GABA (an increase and decrease, respectively), thereby showing riskiness in mitigation of synaptic inhibition by PP and triggering development of neuropathology.

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Section: Methodsmentioning

confidence: 99%

A comparative study of wood sawdust and plastic smoke particulate matter with a focus on spectroscopic, fluorescent, oxidative, and neuroactive properties

Tarasenko

Pozdnyakova

Paliienko

et al. 2022

Environ Sci Pollut Res

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“…Membrane potential of synaptosomes was measured with a fluorescent dye rhodamine 6G (0.5 μM) using its potential-modulated binding to the plasma membrane. 55 The suspension of synaptosomes (0.2 mg/mL of final protein concentration) after preincubation at 37 °C for 30 min with or without DA-induced oligomers was added to the stirred thermostated cuvette. To estimate changes in the plasma membrane potential, the decrease in fluorescence intensity comparing to one at the initial time (F/F 0 ) was calculated.…”

Section: ■ Conclusionmentioning

confidence: 99%

Dopamine-Induced Oligomers of α-Synuclein Inhibit Amyloid Fibril Growth and Show No Toxicity

Galkin

Topcheva

Priss

et al. 2023

ACS Chem. Neurosci.

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Parkinson's disease is characterized by the selective death of dopaminergic neurons in the midbrain and accumulation of amyloid fibrils composed of α-synuclein (αSyn). Current treatment involves approaches that compensate the death of dopaminergic neurons by increasing the dopamine levels in remaining cells. However, dopamine can interact with αSyn and produce oligomeric species which were reported to be toxic in many models. We studied formation of dopamine-induced αSyn oligomers and their effect on the αSyn aggregation. Using the Thioflavin T kinetic assay, we have shown that small oligomers efficiently inhibit αSyn fibrillization by binding to fibril ends and blocking the elongation. Moreover, all the fractions of oligomer species proved to be nontoxic in the differentiated SH-SY5Y cell model and showed negligible neurotoxicity on isolated rat synaptosomes. The observed inhibition is an important insight in understanding of dopamine-enhancing therapy on Parkinson's disease progression and explains the absence of pathology enhancement.

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“…The GABA uptake was terminated in different time intervals (1, 5 and 10 min) by filtering aliquots through the Whatman GF/C filters. After twice washing, with 5 ml the standard salt solution, filters were dried, then were suspended in organic counting scintillant and counted in a Delta 300 (Tracor Analytic, USA) scintillation counter (Pozdnyakova et al 2015 ). Non-specific binding of [ 3 H]GABA was evaluated in cooling samples sedimented immediately after the addition of radiolabeled GABA.…”

Section: Methodsmentioning

confidence: 99%

Effects of surface functionalization of hydrophilic NaYF4 nanocrystals doped with Eu3+ on glutamate and GABA transport in brain synaptosomes

et al. 2017

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Specific rare earth doped nanocrystals (NCs), a recent class of nanoparticles with fluorescent features, have great bioanalytical potential. Neuroactive properties of NaYF4 nanocrystals doped with Eu3+ were assessed based on the analysis of their effects on glutamate- and γ-aminobutyric acid (GABA) transport process in nerve terminals isolated from rat brain (synaptosomes). Two types of hydrophilic NCs were examined in this work: (i) coated by polyethylene glycol (PEG) and (ii) with OH groups at the surface. It was found that NaYF4:Eu3+-PEG and NaYF4:Eu3+-OH within the concentration range of 0.5–3.5 and 0.5–1.5 mg/ml, respectively, did not influence Na+-dependent transporter-dependent l-[14C]glutamate and [3H]GABA uptake and the ambient level of the neurotransmitters in the synaptosomes. An increase in NaYF4:Eu3+-PEG and NaYF4:Eu3+-OH concentrations up to 7.5 and 3.5 mg/ml, respectively, led to the (1) attenuation of the initial velocity of uptake of l-[14C]glutamate and [3H]GABA and (2) elevation of ambient neurotransmitters in the suspension of nerve terminals. In the mentioned concentrations, nanocrystals did not influence acidification of synaptic vesicles that was shown with pH-sensitive fluorescent dye acridine orange, however, decreased the potential of the plasma membrane of synaptosomes. In comparison with other nanoparticles studied with similar methodological approach, NCs start to exhibit their effects on neurotransmitter transport at concentrations several times higher than those shown for carbon dots, detonation nanodiamonds and an iron storage protein ferritin, whose activity can be registered at 0.08, 0.5 and 0.08 mg/ml, respectively. Therefore, NCs can be considered lesser neurotoxic as compared to above nanoparticles.

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New effects of GABAB receptor allosteric modulator rac-BHFF on ambient GABA, uptake/release, Em and synaptic vesicle acidification in nerve terminals

Cited by 13 publications

References 40 publications

A comparative study of wood sawdust and plastic smoke particulate matter with a focus on spectroscopic, fluorescent, oxidative, and neuroactive properties

A comparative study of wood sawdust and plastic smoke particulate matter with a focus on spectroscopic, fluorescent, oxidative, and neuroactive properties

Dopamine-Induced Oligomers of α-Synuclein Inhibit Amyloid Fibril Growth and Show No Toxicity

Effects of surface functionalization of hydrophilic NaYF4 nanocrystals doped with Eu3+ on glutamate and GABA transport in brain synaptosomes

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