New engineered Geobacillus lipase GD-95RM for industry focusing on the cleaner production of fatty esters and household washing product formulations

Druteika, Gytis; Sadauskas, Mikas; Malunavicius, Vilius; Lastauskienė, Eglė; Statkevičiūtė, Roberta; Savickaite, Agne; Gudiukaitė, Renata

doi:10.1007/s11274-020-02816-3

Cited by 8 publications

(1 citation statement)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Generally, this process involves two steps, including first, the construction of the mutant library, which needs to be of adequate size and diversity; the second step involves high-throughput screening, which is fast, sensitive, and directional. Druteika et al used this technique to mutate Geobacillus lipase GD-95 from with the goal of improving enzyme activity; it was identified that the specific activity of GD-95RM lipase increased by 1.3 times (2000 U/mg; Druteika et al, 2020 ).…”

Section: Heterologous Expression and Molecular Modification Of Microbial Lipasesmentioning

confidence: 99%

A Valuable Product of Microbial Cell Factories: Microbial Lipase

Yao

Liu

et al. 2021

Front. Microbiol.

View full text Add to dashboard Cite

As a powerful factory, microbial cells produce a variety of enzymes, such as lipase. Lipase has a wide range of actions and participates in multiple reactions, and they can catalyze the hydrolysis of triacylglycerol into its component free fatty acids and glycerol backbone. Lipase exists widely in nature, most prominently in plants, animals and microorganisms, among which microorganisms are the most important source of lipase. Microbial lipases have been adapted for numerous industrial applications due to their substrate specificity, heterogeneous patterns of expression and versatility (i.e., capacity to catalyze reactions at the extremes of pH and temperature as well as in the presence of metal ions and organic solvents). Now they have been introduced into applications involving the production and processing of food, pharmaceutics, paper making, detergents, biodiesel fuels, and so on. In this mini-review, we will focus on the most up-to-date research on microbial lipases and their commercial and industrial applications. We will also discuss and predict future applications of these important technologies.

show abstract

Section: Heterologous Expression and Molecular Modification Of Microbial Lipasesmentioning

confidence: 99%