The new IntCal20 radiocarbon record continues decades of successful practice by employing one calibration curve as an approximation for different regions across the hemisphere. Here we investigate three radiocarbon time-series of archaeological and historical importance from the Mediterranean-Anatolian region, which indicate, or may include, offsets from IntCal20 (~0-22 14 C years). While modest, these differences are critical for our precise understanding of historical and environmental events across the Mediterranean Basin and Near East. Offsets towards older radiocarbon ages in Mediterranean-Anatolian wood can be explained by a divergence between high-resolution radiocarbon dates from the recent generation of accelerator mass spectrometry (AMS) versus dates from previous technologies, such as low-level gas proportional counting (LLGPC) and liquid scintillation spectrometry (LSS). However, another reason is likely differing growing season lengths and timings, which would affect the seasonal cycle of atmospheric radiocarbon concentrations recorded in different geographic zones. Understanding and correcting these offsets is key to the welldefined calendar placement of a Middle Bronze Age tree-ring chronology. This in turn resolves longstanding debate over Mesopotamian chronology in the earlier second millennium BCE. Last but not least, accurate dating is needed for any further assessment of the societal and environmental impact of the Thera/Santorini volcanic eruption. The 2020 International Northern Hemisphere (NH) Radiocarbon (14 C) Calibration curve, IntCal20, forms the current basis to calendar ages for many scientific fields from 0 to 55 kyr ago 1,2. IntCal20 continues the longstanding assumption that a single 14 C calibration curve is applicable to the mid-latitudes of the NH 1-4. However, there are indications of small, fluctuating, 14 C offsets which, at high-resolution, may affect accurate 14 C-based chronology in some mid-latitude regions 5-10. Part of such differences may result from inter-laboratory offsets (see Supplementary Discussion 1), or derive from differences between recent AMS 14 C measurements versus those from previous 14 C dating technologies. Another part is inferred as a representation of the differing parts of the intra-annual atmospheric 14 C cycle, recorded because of different plant growth seasons or contexts. An example of the latter is the difference between the growth period of tree rings in central and northern Europe and northern America that comprise the Holocene IntCal record (spring through summer), versus those of many