New experimental technique to study blood cardioplegia in the isolated, perfused rat heart

Ozone Administration Reduces Reperfusion Injury in an Isolated Rat Heart Model

“…This is a well-established model permitting studies of various agents on reperfusion injury. 17,18 In our study we could demonstrate that hearts treated with ozone for five minutes during reperfusion had a significantly better recovery than had controls. One may argue that the improvement may be due to improved oxygenation in hearts perfused with ozone.…”

“…We chose the Langendorff isolated rat heart model. This is a well‐established model permitting studies of various agents on reperfusion injury 17,18 . In our study we could demonstrate that hearts treated with ozone for five minutes during reperfusion had a significantly better recovery than had controls.…”

A 31P NMR study of preconditioned isolated perfused rat heart exposed to intermittent ischemia

“…Male Sprague-Dawley rats (350-400 g) were injected with heparin (300 U/rat, intraperitoneally) and anesthetized (pentobarbital, 30 mg/rat, intraperitoneally) 30 min before the removal of the heart. The excised hearts were immediately immersed in an ice-cold saline solution and quickly mounted, through their aorta, onto a Langendorff perfusion system adapted for use with the NMR apparatus (18). The pulmonary artery was cut open in each heart to guarantee unrestricted coronary flow.…”