2010
DOI: 10.1002/chem.200903272
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New Fluorinated Rhodamines for Optical Microscopy and Nanoscopy

Abstract: New photostable rhodamine dyes represented by the compounds 1 a-r and 3-5 are proposed as efficient fluorescent markers with unique combination of structural features. Unlike rhodamines with monoalkylated nitrogen atoms, N',N-bis(2,2,2-trifluoroethyl) derivatives 1 e, 1 i, 1 j, 3-H and 5 were found to undergo sulfonation of the xanthene fragment at the positions 4' and 5'. Two fluorine atoms were introduced into the positions 2' and 7' of the 3',6'-diaminoxanthene fragment in compounds 1 a-d, 1 i-l and 1 m-r. … Show more

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Cited by 107 publications
(97 citation statements)
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“…These include long-established STED fluorophores such as Chromeo 488, Atto 565, and Atto 647 N, as well the recent rhodamine derivatives, carbopyronine derivatives, or Abberior STAR series fluorophores which are now commercially available (e.g., Abberior STAR 635P) [34,52,81,82,99,100]. Multicolor imaging strategies include the use of common excitation and depletion wavelengths with closely emitting fluorophores (e.g., that can be separated through spectral unmixing techniques, as was described above for two-color fluorescent protein imaging).…”
Section: Organic Fluorophores For Super-resolution Imagingmentioning
confidence: 99%
“…These include long-established STED fluorophores such as Chromeo 488, Atto 565, and Atto 647 N, as well the recent rhodamine derivatives, carbopyronine derivatives, or Abberior STAR series fluorophores which are now commercially available (e.g., Abberior STAR 635P) [34,52,81,82,99,100]. Multicolor imaging strategies include the use of common excitation and depletion wavelengths with closely emitting fluorophores (e.g., that can be separated through spectral unmixing techniques, as was described above for two-color fluorescent protein imaging).…”
Section: Organic Fluorophores For Super-resolution Imagingmentioning
confidence: 99%
“…Therefore, a low probability of excited-state absorption at the wavelength of the STED laser is one of the priorities when designing novel fluorescent labels optimized for STED nanoscopy. A whole palette of fluorescent labels has been designed throughout recent years [75][76][77] ranging from organic dyes for immuno- [75] and related labeling approaches [78] or for live-cell tagging using the Halo, Snap or Clip and similar technologies [35,36,[79][80][81], and similarly fluorescent proteins for common live-cell STED recordings [32,37].…”
Section: Optimization For Cellular Imagingmentioning
confidence: 99%
“…The red emitting dyes are of interest in the area of OLED [1][2][3][4], protein tracking [5], multicolor imaging [6], far-field optical nanoscopy [7,8]. The red emitting dyes are important in OLEDs to complement their blue [9,10] and green [11,12] dye counterparts in fabrication of displays.…”
Section: Introductionmentioning
confidence: 99%