New Foldback transposable element TFB1 found in histone genes of the midge Chironomus thummi

Hankeln, T; Schmidt, Erwin R.

doi:10.1016/s0022-2836(05)80159-7

Cited by 26 publications

(15 citation statements)

References 29 publications

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“…However, limited similarity may be noted between TIRs of PrFT, the FB elements of Drosophila melanogaster, and TFB1 from Chironomus thummi for which the corresponding TPases have not been identified (Fig. 2C) (17,18). The absence of identifiable coding sequences complicates determination of the current mobility status of foldback TEs in bdelloids.…”

Section: Resultsmentioning

confidence: 99%

Diverse DNA transposons in rotifers of the class Bdelloidea

Arkhipova

Meselson

2005

Proc. Natl. Acad. Sci. U.S.A.

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We surveyed the diversity, structural organization, and patterns of evolution of DNA transposons in rotifers of the class Bdelloidea, a group of basal triploblast animals that appears to have evolved for millions of years without sexual reproduction. Representatives of five superfamilies were identified: ITm (IS630͞Tc͞mariner), hAT, piggyBac, helitron, and foldback. Except for mariners, no fully intact copies were found. Mariners, both intact and decayed, are present in high copy number, and those described here may be grouped in several closely related lineages. Comparisons across lineages show strong evidence of purifying selection, whereas there is little or no evidence of such selection within lineages. This pattern could have resulted from repeated horizontal transfers from an exogenous source, followed by limited intragenomic proliferation, or, less plausibly, from within-host formation of new lineages under host-or element-based selection for function, in either case followed by eventual inactivation and decay. Unexpectedly, the flanking sequences surrounding the majority of mariners are very similar, indicating either insertion specificity or proliferation as part of larger DNA segments. Members of all superfamilies are present near chromosome ends, associated with the apparently domesticated retroelement Athena, in large clusters composed of diverse DNA transposons, often inserted into each other, whereas the examined gene-rich regions are nearly transposon-free.foldback ͉ hAT ͉ helitron ͉ mariner ͉ piggyBac

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Section: Resultsmentioning

confidence: 99%

Diverse DNA transposons in rotifers of the class Bdelloidea

Arkhipova

Meselson

2005

Proc. Natl. Acad. Sci. U.S.A.

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“…These new Galileo copies are basically composed of very long ITRs, with a relatively small and heterogeneous central region that does not seem to encode any protein involved in their transposition. Like the first two copies, they do not show homology to any known sequence in the available databases, but they display significant structural similarity to the Foldback elements described in many organisms (Bingham and Zachar 1989;Hoffman-Liebermann et al 1989;Hankeln and Schmidt 1990;Yuan et al 1991;Rebatchouk and Narita 1997), including the ability to form stable secondary structures when denatured (as indicated by the difficulties encountered in the PCR amplification of the segments containing these elements). Five other insertions corresponding to two closely related TEs (average sequence identity 84%) also show similarities to Foldback elements.…”

Section: Structural Variation At Inversion 2j Breakpoint Regionsmentioning

confidence: 99%

Molecular Characterization of Two Natural Hotspots in the Drosophila buzzatii Genome Induced by Transposon Insertions

Cáceres¹,

Puig²,

Ruíz³

2001

Genome Res.

104

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Transposable elements (TEs) have been implicated in the generation of genetic rearrangements, but their potential to mediate changes in the organization and architecture of host genomes could be even greater than previously thought. Here, we describe the naturally occurring structural and nucleotide variation around two TE insertions in the genome of Drosophila buzzatii. The studied regions correspond to the breakpoints of a widespread chromosomal inversion generated by ectopic recombination between oppositely oriented copies of a TE namedGalileo. A detailed molecular analysis by Southern hybridization, PCR amplification, and DNA sequencing of 7.1 kb surrounding the inversion breakpoints in 39 D. buzzatii lines revealed an unprecedented degree of restructuring, consisting of 22 insertions of ten previously undescribed TEs, 13 deletions, 1 duplication, and 1 small inversion. All of these alterations occurred exclusively in inverted chromosomes and appear to have accumulated after the insertion of the Galileo elements, within or close to them. The nucleotide variation at the studied regions is six times lower in inverted than in noninverted chromosomes, suggesting that most of the observed changes originated in only 84,000 years.Galileo elements thus seemed to promote the transformation of these, otherwise normal, chromosomal regions in genetically unstable hotspots and highly efficient traps for transposon insertions. The particular features of two new Galileo copies found indicate that this TE belongs to the Foldback family. Together, our results strengthen the importance of TEs, and especially DNA transposons, as inducers of genome plasticity in evolution.[The sequence data described in this paper have been submitted to the GenBank data library under accession nos.AF368842–AF368859 and AF368861–AF368900. In addition, sequences submitted under accession nos. AF162796–AF162799 were used as a basis for this study.]

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“…The FB element is supposed to be a DNA-type transposable element (Potter, 1982a). FB-like transposable elements with TIRs consisting of tandem repeats have been identified in other organisms: TU in sea urchin (Hoffman-Liebermann et al, 1985), TFB1 in Chiromomus thummi (Hankeln and Schmidt, 1990), and SoFT in Solanaceae plants (Rebatchouk and Narita, 1997).…”

Section: Introductionmentioning

confidence: 99%

Tnr8, a foldback transposable element from rice.

et al. 2000

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An insertion sequence 418 bp in length was found in one member of rice retroposon p-SINE1 in Oryza glaberrima. This sequence had long terminal inverted repeats (TIRs) and is flanked by direct repeats of a 9-bp sequence at the target site, indicative that the insertion sequence is a rice transposable element, which we named Tnr8. Interestingly, each TIR sequence consisted of a unique 9-bp terminal sequence and six tandem repeats of a sequence about 30 bp in length, like the foldback transposable element first identified in Drosophila. A homology search of databases and analysis by PCR revealed that a large number of Tnr8 members with sequence variations were present in the rice genome. Some of these members were not present at given loci in several rice species with the AA genome. These findings suggest that the Tnr8 family members transposed long ago, but some appear to have mobilized after rice strains with the AA genome diverged. The Tnr8 members are thought to be involved in rearrangements of the rice genome.

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New Foldback transposable element TFB1 found in histone genes of the midge Chironomus thummi

Cited by 26 publications

References 29 publications

Diverse DNA transposons in rotifers of the class Bdelloidea

Diverse DNA transposons in rotifers of the class Bdelloidea

Molecular Characterization of Two Natural Hotspots in the Drosophila buzzatii Genome Induced by Transposon Insertions

Tnr8, a foldback transposable element from rice.

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