New frontiers in oilseed biotechnology: meeting the global demand for vegetable oils for food, feed, biofuel, and industrial applications

Lu, Chaofu; Napier, Johnathan A.; Clemente, Thomas E.; Cahoon, Edgar B.

doi:10.1016/j.copbio.2010.11.006

Cited by 237 publications

(186 citation statements)

References 55 publications

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“…gov/oilseeds/). There is a pressing need to improve the yield of oil crops to meet the world's growing demand for renewable oils (Lu et al, 2011). It has recently been proposed that a step change in oil yield may be achievable in terrestrial crops if they can be engineered to produce oil in vegetative tissues rather than in seeds (Durrett et al, 2008;Ohlrogge et al, 2009).…”

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confidence: 99%

The SUGAR-DEPENDENT1 Lipase Limits Triacylglycerol Accumulation in Vegetative Tissues of Arabidopsis

et al. 2013

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There has been considerable interest recently in the prospect of engineering crops to produce triacylglycerol (TAG) in their vegetative tissues as a means to achieve a step change in oil yield. Here, we show that disruption of TAG hydrolysis in the Arabidopsis (Arabidopsis thaliana) lipase mutant sugar-dependent1 (sdp1) leads to a substantial accumulation of TAG in roots and stems but comparatively much lower TAG accumulation in leaves. TAG content in sdp1 roots increases with the age of the plant and can reach more than 1% of dry weight at maturity, a 50-fold increase over the wild type. TAG accumulation in sdp1 roots requires both ACYL-COENZYME A:DIACYLGLYCEROL ACYLTRANSFERASE1 (DGAT1) and PHOSPHATIDYLCHOLINE: DIACYLGLYCEROL ACYLTRANSFERASE1 and can also be strongly stimulated by the provision of exogenous sugar. In transgenic plants constitutively coexpressing WRINKLED1 and DGAT1, sdp1 also doubles the accumulation of TAG in roots, stems, and leaves, with levels ranging from 5% to 8% of dry weight. Finally, provision of 3% (w/v) exogenous Suc can further boost root TAG content in these transgenic plants to 17% of dry weight. This level of TAG is similar to seed tissues in many plant species and establishes the efficacy of an engineering strategy to produce oil in vegetative tissues that involves simultaneous manipulation of carbohydrate supply, fatty acid synthesis, TAG synthesis, and also TAG breakdown.

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The SUGAR-DEPENDENT1 Lipase Limits Triacylglycerol Accumulation in Vegetative Tissues of Arabidopsis

et al. 2013

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“…Plant oils are an important source of energy for human and animal diets and are regularly used in production of biofuels and industrial products (Lu et al, 2011). Therefore, there is considerable effort to increase the oil quality and yield in plants through breeding and application of biotechnology (Tan et al, 2011;Shi et al, 2012;Haslam et al, 2013;Vanhercke et al, 2013;van Erp et al, 2014;Li et al, 2015a).…”

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confidence: 99%

“…Camelina was chosen for obvious advantages-it can be floral-dip transformed (Lu et al, 2011); it has a short lifecycle; and it has oil-rich seeds (30% to 40% of seed weight) that contain high levels of PUFAs (.50%), especially a-linolenic acid (18:3, .30% of total fatty acids; Campbell et al, 2013;Iskandarov et al, 2013). Recently phospholipase A overexpression in Camelina resulted in an increase in total oil (Li et al, 2015a), and other transgenics have resulted in TAG containing nonnative FAs (Lu and Kang, 2008;Petrie et al, 2014;Ruiz-Lopez et al, 2014;Liu et al, 2015;Nguyen et al, 2015).…”

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confidence: 99%

Phospholipase Dζ Enhances Diacylglycerol Flux into Triacylglycerol

Yang

Wang

et al. 2017

Plant Physiol.

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Plant seeds are the primary source of triacylglycerols (TAG) for food, feed, fuel, and industrial applications. As TAG is produced from diacylglycerol (DAG), successful engineering strategies to enhance TAG levels have focused on the conversion of DAG to TAG. However, the production of TAG can be limited by flux through the enzymatic reactions that supply DAG. In this study, two Arabidopsis phospholipase D z genes (AtPLD z1 and AtPLD z2 ) were coexpressed in Camelina sativa to test whether the conversion of phosphatidylcholine to DAG impacts TAG levels in seeds. The resulting transgenic plants produced 2% to 3% more TAG as a component of total seed biomass and had increased 18:3 and 20:1 fatty acid levels relative to wild type. Increased DAG and decreased PC levels were examined through the kinetics of lipid assembly by [ 14 C]acetate and [ 14 C]glycerol incorporation into glycerolipids. [ 14 C]acetate was rapidly incorporated into TAG in both wild-type and overexpression lines, indicating a significant flux of nascent and elongated acyl-CoAs into the sn-3 position of TAG. Stereochemical analysis revealed that newly synthesized fatty acids were preferentially incorporated into the sn-2 position of PC, but the sn-1 position of de novo DAG and indicated similar rates of nascent acyl groups into the Kennedy pathway and acyl editing. [ 14 C]glycerol studies demonstrated PC-derived DAG is the major source of DAG for TAG synthesis in both tissues. The results emphasize that the interconversions of DAG and PC pools can impact oil production and composition.

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“…The volume of vegetable oil that is produced globally has increased very substantially in recent decades and now is in excess of 150 million metric tons per year (http://www.fas.usda.gov/data/ oilseeds-world-markets-and-trade). There is an urgent need to improve the yield of oil crops to meet societies' ever-growing demand for this renewable feedstock (Lu et al, 2011). The genetic architecture of yield in oilseed crops is complex (Würschum et al, 2012).…”

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Multigene Engineering of Triacylglycerol Metabolism Boosts Seed Oil Content in Arabidopsis

et al. 2014

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Increasing the yield of oilseed crops is an important objective for biotechnologists. A number of individual genes involved in triacylglycerol metabolism have previously been reported to enhance the oil content of seeds when their expression is altered. However, it has yet to be established whether specific combinations of these genes can be used to achieve an additive effect and whether this leads to enhanced yield. Using Arabidopsis (Arabidopsis thaliana) as an experimental system, we show that seed-specific overexpression of WRINKLED1 (a transcriptional regulator of glycolysis and fatty acid synthesis) and DIACYLGLYCEROL ACYLTRANSFERASE1 (a triacylglycerol biosynthetic enzyme) combined with suppression of the triacylglycerol lipase SUGAR-DEPENDENT1 results in a higher percentage seed oil content and greater seed mass than manipulation of each gene individually. Analysis of total seed yield per plant suggests that, despite a reduction in seed number, the total yield of oil is also increased.

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New frontiers in oilseed biotechnology: meeting the global demand for vegetable oils for food, feed, biofuel, and industrial applications

Cited by 237 publications

References 55 publications

The SUGAR-DEPENDENT1 Lipase Limits Triacylglycerol Accumulation in Vegetative Tissues of Arabidopsis

The SUGAR-DEPENDENT1 Lipase Limits Triacylglycerol Accumulation in Vegetative Tissues of Arabidopsis

Phospholipase Dζ Enhances Diacylglycerol Flux into Triacylglycerol

Multigene Engineering of Triacylglycerol Metabolism Boosts Seed Oil Content in Arabidopsis

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