New generation of peptide antibiotics.

Dubin, Adam; Mak, Paweł; Dubin, Grzegorz; Rzychon, Małgorzata; Steć-Niemczyk, Justyna; Władyka, Benedykt; Maziarka, Katarzyna; Chmiel, Dorota

doi:10.18388/abp.2005_3423

Cited by 21 publications

(13 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The function of a factor of virulence in pathogenic microorganisms lowering the vulnerability to the defence mechanisms of the hosts. Important for both Pro-and Eukaryotic pathogens, it draws one's attention in quest of specific targeting of individual microbial virulence factors as an alternative antimicrobial strategy (Dubin et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Melanin synthesis in microorganisms--biotechnological and medical aspects.

Płonka¹,

Grabacka²

2006

Acta Biochim Pol

289

197

View full text Add to dashboard Cite

Melanins form a diverse group of pigments synthesized in living organisms in the course of hydroxylation and polymerization of organic compounds. Melanin production is observed in all large taxa from both Pro- and Eukaryota. The basic functions of melanins are still a matter of controversy and speculation, even though their adaptative importance has been proved. Melanogenesis has probably evolved parallel in various groups of free living organisms to provide protection from environmental stress conditions, but in pathogenic microorganisms it correlates with an increased virulence. The genes responsible for melanization are collected in some cases within operons which find a versatile application in genetic engineering. This review summarizes current views on melanogenesis in Pro- and Eukaryotic microorganisms in terms of their biotechnological and biomedical importance.

show abstract

Section: Discussionmentioning

confidence: 99%

Melanin synthesis in microorganisms--biotechnological and medical aspects.

Płonka¹,

Grabacka²

2006

Acta Biochim Pol

289

197

View full text Add to dashboard Cite

show abstract

“…As AMPs begin showing their potential as highly selective antitumor drugs, even against diseases resistant to chemotherapy (Dubin et al, 2005;Mader & Hoskin, 2006), this potential should be taken advantage of to solve the main problems in conventional anti-cancer chemotherapy: toxic side-effects, low response of slowgrowing tumors and dormant cells (Naumov et al, 2003) and the development of multi-drug resistance by tumor cells (Gottesman, 2002) even against anti-angiogenic drugs (Ferrara & Kerbel, 2005). Understanding the relationships between AMPs structure and antitumor activity would be helpful in the development of synthetic peptides with the highest antitumor activity and selectivity and low side-effects, e.g., haemolysis.…”

Section: Tnf-α Release In Supernatants Of U937 Cell Line Treated Withmentioning

confidence: 99%

“…Antimicrobial peptides (AMPs) constitute an evolutionarily conserved part of the innate immune defense mechanism found in a wide range of prokaryotic and eukaryotic organisms. They are active against bacteria, fungi, viruses and also take part in anti-tumor response making them promising candidates for therapeutic use (Lehrer & Ganz, 1999;Reddy et al, 2004;Dubin et al, 2005). Over 1000 AMPs have already been isolated and characterized at the level of their primary structure (Bulet et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Antitumor activity of antimicrobial peptides against U937 histiocytic cell line.

Koszałka

Kamysz²,

Wejda³

et al. 2011

Acta Biochim Pol

View full text Add to dashboard Cite

We investigated cytotoxic activity of antimicrobial peptides of different origin (both naturally occurring and synthetic), structure and known mechanisms of action against human histiocytic lymphoma cell line U937. The strongest cytotoxic activity against U937 cell line was shown by Pexiganan MSI-78, followed by Citropin 1.1, Protegrin 1 and a synthetic lipopeptide, N-α-palmitoyl-L-lysyl-L-lysine amide (Pal-Lys-Lys-NH₂). The cytotoxic activity of the peptides was more dependent on the time of incubation than concentration. Only for the lipopeptide, whose mode of action was restricted to disruption of electric potential of the cell membrane, the correlation between cytotoxicity and concentration was almost linear. The high cytotoxicity of Pexiganan MSI-78, Protegrin 1 and the lipopeptide could be basically explained by their membranolytic activity leading to necrosis. However, in the case of Citropin 1.1, the cell membrane integrity was disrupted only slightly and independently of the peptide concentration. Therefore, some other mechanism of action might be responsible for its strong dose-dependent cytotoxic activity, e.g., membranolytic activity leading to apoptosis. Furthermore, TNF-α production due to LPS (lipopolysaccharide) stimulation was suppressed by the presence of Citropin 1.1, Pexiganan MSI-78 or Protegrin 1, but not by Buforin 2 or the lipopeptide. Our experiments have shown that cytotoxic activity is not limited to some specific molecular structure of a peptide, but rather to the length of the peptide chain as it is likely to affect the efficiency of the tumor cell membrane disruption and interaction with LPS.

show abstract

“…Antimicrobial peptides are found in a variety of organisms and are considered the future of anti-infective therapy drugs. Antimicrobial peptides have active smallmolecule peptides with broad-spectrum antimicrobial activity and are a first line of defense for various living organisms, 6,7 with the advantages of high efficiency, broad spectrum, and little drug resistance.…”

Section: Introductionmentioning

confidence: 99%

Human Beta-Defensin-3 for the Diagnosis of Periprosthetic Joint Infection and Loosening

Liu¹,

Yu²,

Ou³

et al. 2014

Orthopedics

View full text Add to dashboard Cite

In this study, the difference in expression of human beta-defensin-3 in periprosthetic tissue and cancellous bone was observed in the periprosthetic tissue and cancellous bone of patients in the periprosthetic joint infection group, the aseptic loosening group, and the spacer treatment group as well as the synovial membrane and ilium of the normal control group. Hematoxylin and eosin staining of the synovial tissue showed different levels of neutrophil infiltration in all groups except for the normal control group. Immunofluorescence staining of periprosthetic tissue and cancellous bone showed the most positive cells expressing human beta-defensin-3 and the largest mean optical density in the periprosthetic joint infection group, followed by the aseptic loosening group, the spacer treatment group, and the normal control group, with a significant difference in comparison between the periprosthetic joint infection group and the other 3 groups (P<.01). White blood cell count, erythrocyte sedimentation rate, and C-reactive protein level were highest in the periprosthetic joint infection group, whereas no difference was found between the other 3 groups. Taken together, high levels of human beta-defensin-3 protein expression were found in the periprosthetic tissue and cancellous bone of patients with periprosthetic joint infection and aseptic loosening, but there are differential expressions of human beta-defensin-3, and this may provide a new marker for the differential diagnosis of infection and loosening of the artificial joint.

show abstract

New generation of peptide antibiotics.

Cited by 21 publications

References 34 publications

Melanin synthesis in microorganisms--biotechnological and medical aspects.

Melanin synthesis in microorganisms--biotechnological and medical aspects.

Antitumor activity of antimicrobial peptides against U937 histiocytic cell line.

Human Beta-Defensin-3 for the Diagnosis of Periprosthetic Joint Infection and Loosening

Contact Info

Product

Resources

About