New hepatitis C virus (HCV) genotyping system that allows for identification of HCV genotypes 1a, 1b, 2a, 2b, 3a, 3b, 4, 5a, and 6a

Ohno, O; Mizokami, Masashi; Wu, Rongrong; Saleh, Mohamed; Ohba, Ken Ichi; Orito, Etsuro; Mukaide, Motokazu; Williams, Roger L.; Lau, Johnson Y.N.

doi:10.1128/jcm.35.1.201-207.1997

Cited by 431 publications

(244 citation statements)

References 43 publications

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“…The HCV genotypes were identified by PCR with combinations of genotype-specific primers, targeting the core region as previously described. (Ohno et al, 1997) Treatment and monitoring. Patients were treated with a combination of interferon and ribavirin for 12 months.…”

Section: Methodsmentioning

confidence: 99%

Interferon and ribavirin as frontline treatment for chronic hepatitis C infection in thalassaemia major

Li¹,

Chan

Ling

et al. 2002

Br J Haematol

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Summary. Hepatitis C virus (HCV) infection is common in transfusion-dependent thalassaemia. The clinical usefulness of 12-month treatment, using interferon alpha 3 MIU/m 2 thrice weekly and oral ribavirin 16 mg/kg/d, was evaluated in 18 previously untreated thalassaemia patients. The median age at start of treatment was 16 years (range 7-29). Fourteen were infected with genotype 1b and 4 with genotype 6a. The sustained biochemical and virological response rates 6 months after stopping treatment were both 72AE2%. Blood consumption was temporarily increased by 30% due to ribavirin-associated haemolysis. This study demonstrated a high, sustained response rate to combination treatment despite infection with genotype 1b.

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Section: Methodsmentioning

confidence: 99%

Interferon and ribavirin as frontline treatment for chronic hepatitis C infection in thalassaemia major

Li¹,

Chan

Ling

et al. 2002

Br J Haematol

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“…Total RNA was extracted from the serum samples using the SepaGene RV-R Nucleic acid extraction kit (Sanko Junyaku Co., Ltd, Tokyo, Japan) in accordance with the manufacturer's protocol. Viral RNA were reverse transcribed to complementary DNA using SuperScript II RNase H_Reverse Transcriptase (Invitrogen Corp., Carlsbad, CA) and random hexamer primer (Takara Shuzo Co. Ltd, Tokyo, Japan) as described previously [Ohno et al, 1997]. Screening of HEV RNA was carried out with a specific set of screening primers targeting a partial nucleotide sequence of ORF1 region of the HEV genome, as described previously [Takahashi et al, 2003].…”

Section: Detection Of Hev Rna and Hev Genotypingmentioning

confidence: 99%

Investigating an outbreak of acute viral hepatitis caused by hepatitis E virus variants in Karachi, South Pakistan

et al. 2011

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Hepatitis E is a classic water-borne disease in developing countries. Detection of anti-HEV IgM and IgG antibodies, in addition to HEV RNA are useful epidemiological markers in diagnosis of hepatitis E. This study was conducted to investigate an outbreak of acute viral hepatitis in South-Pakistan. Anti-HEV IgM and IgG were assessed comparatively with serological kits manufactured by Abbott, Cosmic, TGH, and Wantai, selecting HEV RNA as reference assay. Molecular evolutionary analysis was performed by phylogeny and HEV spread time analysis by Bayesian Coalescent Theory approach. Of the 89 patients, 24 (26.9%) did not have acute hepatitis viral marker. Of the remaining 65 cases, 4 (6.1%) were positive for anti-HAV IgM, one (1.5%) for anti-HBc IgM, 2 (3%) for HCV, 53 (81.5%) for anti-HEV IgM, and 5 (7.7%) were hepatitis-negative. The Wantai test was 100% sensitive and specific followed by Cosmic (98.1% and 100%), TGH (98.1% and 97.2%) and Abbott (79.2% and 83.3%). Two HEV variant strains were detected by phylogeny responsible for this acute hepatitis outbreak. Estimates on demographic history of HEV showed that HEV in Pakistan has remained at a steady nonexpanding phase from around 1970 to the year 2005, in which it expanded explosively with the emergence of new HEV variants. In conclusion, the limited sensitivity of available assay (Abbott anti-HEV EIA) may be a concern in HEV diagnosis in Pakistan. This study cautions that the dissemination of the variant strains to other areas of Pakistan may lead to explosive HEV outbreaks.

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“…Reverse transcription polymerase chain reaction (RT-PCR) and nested PCR of core genome were performed according to the method of Ohno et al 25 with minor modification. Briefly, extracted RNA was first amplified by RT-PCR with outer primers Sc2 and Ac2 at a portion of core region.…”

Section: Rt-pcr and Nested Pcr For Core Regionmentioning

confidence: 99%

Hepatitis C virus genotype distribution in Myanmar: Predominance of genotype 6 and existence of new genotype 6 subtype

Lwin

Shinji²,

Khin

et al. 2007

Hepatology Research

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New hepatitis C virus (HCV) genotyping system that allows for identification of HCV genotypes 1a, 1b, 2a, 2b, 3a, 3b, 4, 5a, and 6a

Cited by 431 publications

References 43 publications

Interferon and ribavirin as frontline treatment for chronic hepatitis C infection in thalassaemia major

Interferon and ribavirin as frontline treatment for chronic hepatitis C infection in thalassaemia major

Investigating an outbreak of acute viral hepatitis caused by hepatitis E virus variants in Karachi, South Pakistan

Hepatitis C virus genotype distribution in Myanmar: Predominance of genotype 6 and existence of new genotype 6 subtype

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