New Highly Sensitive Methods for Electroanalysis of the Catalytic Activity of Enzymes of Medical Significance

Abstract

The review is devoted to new highly effective methods for analyzing the catalytic activity of enzymes of medical significance, such as cytochromes P450, trypsin, asparaginase, beta-lactamase, and nucleases. The methods are based on registration the specific activity of enzymes using electroanalytical methods. The review analyzes the experimental data obtained by the authors. Two platforms have been developed that allow quantitative measurement of catalytic activity based on the electrochemical properties of the enzyme (cytochrome P450, bactosomes, asparaginase) or substrate (trypsin, nucleases, restriction enzymes, beta-lactamase).