New horizons in developing cell lysis methods: A review

Danaeifar, Mohsen

doi:10.1002/bit.28198

Cited by 35 publications

(15 citation statements)

References 109 publications

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“…To prepare cell extracts, several methods, such as mechanical, physical, chemical and biological techniques, have been established for lysing cells. Each technique applies a unique approach to breaking down the cell membrane and has its own set of advantages and disadvantages [ 42 , 43 ]. The freeze–thaw method is a type of physical technique, which creates thermodynamic instability and causes the disruption of cell membrane.…”

Section: Discussionmentioning

confidence: 99%

The extracts of osteoblast developed from adipose-derived stem cell and its role in osteogenesis

Tangporncharoen,

Silathapanasakul,

Tragoonlugkana

et al. 2024

J Orthop Surg Res

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Cell-based therapy has become an achievable choice in regenerative medicines, particularly for musculoskeletal disorders. Adipose-derived stem cells (ASCs) are an outstanding resource because of their ability and functions. Nevertheless, the use of cells for treatment comes with difficulties in operation and safety. The immunological barrier is also a major limitation of cell therapy, which can lead to unexpected results. Cell-derived products, such as cell extracts, have gained a lot of attention to overcome these limitations. The goal of this study was to optimize the production of ASC-osteoblast extracts as well as their involvement in osteogenesis. The extracts were prepared using a freeze–thaw method with varying temperatures and durations. Overall, osteogenic-associated proteins and osteoinductive potential of the extracts prepared from the osteogenic-induced ASCs were assessed. Our results demonstrated that the freeze–thaw approach is practicable for cell extracts production, with minor differences in temperature and duration having no effect on protein concentration. The ASC-osteoblast extracts contain a significant level of essential specialized proteins that promote osteogenicity. Hence, the freeze–thaw method is applicable for extract preparation and ASC-osteoblast extracts may be beneficial as an optional facilitating biologics in bone anabolic treatment and bone regeneration.

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Section: Discussionmentioning

confidence: 99%

The extracts of osteoblast developed from adipose-derived stem cell and its role in osteogenesis

Tangporncharoen,

Silathapanasakul,

Tragoonlugkana

et al. 2024

J Orthop Surg Res

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“…Future efforts could include optimizing the RPA CRISPR/Cas12a biosensor for the detection of Pd from guano without the need of a commercial DNA extraction kit to reduce cost per sample. Bead‐beating cell lysis protocols (Danaeifar, 2022) could be tested in conjunction with nuclease inactivation treatment, lyticase enzyme and a rapid extraction reagent like PrepMan Ultra. Efforts could also survey additional types of noninvasive samples, including further diagnostic surveys of guano and other Pd biotic or abiotic reservoirs that would not disturb bats (e.g.…”

Section: Discussionmentioning

confidence: 99%

A minimally invasive, field‐applicable CRISPR/Cas biosensor to aid in the detection of Pseudogymnoascus destructans, the causative fungal agent of white‐nose syndrome in bats

Pérez,

Tobin,

Stechly

et al. 2023

Molecular Ecology Resources

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The accessibility to CRISPR/Cas (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR‐associated protein) genetic tools has given rise to applications beyond site‐directed genome editing for the detection of DNA and RNA. These tools include precise diagnostic detection of human disease pathogens, such as SARS‐CoV‐2 and Zika virus. Despite the technology being rapid and cost‐effective, the use of CRISPR/Cas tools in the surveillance of the causative agents of wildlife diseases has not been prominent. This study presents the development of a minimally invasive, field‐applicable and user‐friendly CRISPR/Cas‐based biosensor for the detection of Pseudogymnoascus destructans (Pd), the causative fungal agent of white‐nose syndrome (WNS), an infectious disease that has killed more than five million bats in North America since its discovery in 2006. The biosensor assay combines a recombinase polymerase amplification (RPA) step followed by CRISPR/Cas12a nuclease cleavage to detect Pd DNA from bat dermal swab and guano samples. The biosensor had similar detection results when compared to quantitative PCR in distinguishing Pd‐positive versus negative field samples. Although bat dermal swabs could be analysed with the biosensor without nucleic acid extraction, DNA extraction was needed when screening guano samples to overcome inhibitors. This assay can be applied to help with more rapid delineation of Pd‐positive sites in the field to inform management decisions. With further optimization, this technology has broad translation potential to wildlife disease‐associated pathogen detection and monitoring applications.

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“…For non-nuclear cells, simpler extraction methods, such as hypotonic lysis and freeze-thawing, can be used. For nuclear cells, other methods, such as the use of ultrasonic waves and homogenization, are more suitable [24,49]. In industrial-scale production, homogenization combined with centrifugation is extensively used [37,38,49].…”

Section: Cell Membrane Extractionmentioning

confidence: 99%

Cell membrane-coated nanoparticles: a novel multifunctional biomimetic drug delivery system

Liu

Jiang

et al. 2022

Drug Deliv. and Transl. Res.

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Recently, nanoparticle-based drug delivery systems have been widely used for the treatment, prevention, and detection of diseases. Improving the targeted delivery ability of nanoparticles has emerged as a critical issue that must be addressed as soon as possible. The bionic cell membrane coating technology has become a novel concept for the design of nanoparticles. The diverse biological roles of cell membrane surface proteins endow nanoparticles with several functions, such as immune escape, long circulation time, and targeted delivery; therefore, these proteins are being extensively studied in the fields of drug delivery, detoxification, and cancer treatment. Furthermore, hybrid cell membrane-coated nanoparticles enhance the beneficial effects of monotypic cell membranes, resulting in multifunctional and efficient delivery carriers. This review focuses on the synthesis, development, and application of the cell membrane coating technology and discusses the function and mechanism of monotypic/hybrid cell membrane-modified nanoparticles in detail. Moreover, it summarizes the applications of cell membranes from different sources and discusses the challenges that may be faced during the clinical application of bionic carriers, including their production, mechanism, and quality control. We hope this review will attract more scholars toward bionic cell membrane carriers and provide certain ideas and directions for solving the existing problems. Graphical abstract

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New horizons in developing cell lysis methods: A review

Cited by 35 publications

References 109 publications

The extracts of osteoblast developed from adipose-derived stem cell and its role in osteogenesis

The extracts of osteoblast developed from adipose-derived stem cell and its role in osteogenesis

A minimally invasive, field‐applicable CRISPR/Cas biosensor to aid in the detection of Pseudogymnoascus destructans, the causative fungal agent of white‐nose syndrome in bats

Cell membrane-coated nanoparticles: a novel multifunctional biomimetic drug delivery system

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