New HPLC Method with Experimental Design and Fluorescence Detection for Analytical Study of Antihypertensive Mixture, Amlodipine and Valsartan

Abstract

<p class="MsoNormal"> <span lang="EN-US">New HPLC method was developed for determination of amlodipine and valsartan in their binary mixture as a part of routine control of combined formulations. The method was validated to meet official requirements including selectivity, stability, linearity, precision and accuracy. Chromatography was carried out using a LiChrospher RP-18 column, a mixture containing acetonitrile, phosphate buffer of pH 3.5 and methanol (45:45:10, <i>v</i>/<i>v</i>/<i>v</i>) and new fluorescence detec</span><span lang="EN-US">tion at 255 nm for excitation and 448 nm for emission. The effect of methanol content, pH of the buffer, flow rate, de</span><span lang="EN-US">tection wavelengths and column temperature was estimated in robustness study, according to a plan defined by the Plackett-Burman design. For identification of significant effects, both graphical and statistical methods were used. Ro</span><span lang="EN-US">-</span><span lang="EN-US">bustness for dissolution test was checked estimating the effects of paddle speed, temperature and pH of dissolution me</span><span lang="EN-US">dium. The method was proved to complying with all official guidelines. Therefore, it is suitable for determination of amlodipine and valsartan in their binary mixtures for different analytical and pharmaceutical purposes.</span> </p

Electroanalytical application of a boron-doped diamond electrode: Improving the simultaneous voltammetric determination of amlodipine and valsartan in urine and combined dosage forms

“…With the cathodic pretreatment, the BDD electrode surface is made predominantly hydrogen terminated [39][40][41]. The electroactive area of cathodically pretreated BDD electrode (0.117 cm 2 ) was determined from the slope of plot I ap vs v 1/2 , according to Randles-Sevcik equation, using 5.0 mmol L -1 K 4 Fe(CN) 6 The HPLC determinations of AML and VAL were carried out using an LC Thermo Electron Corporation Finnigan Surveyor PDA Plus and ACE 5 C18 column (250 x 4.6 mm, 5 µm).…”

“…Several analytical methods to determine simultaneously AML and VAL in pharmaceutical formulations and biological fluids have been reported in the literature, including those capillary electrophoresis [3], chromatography [4][5][6][7][8][9][10], spectrofluorimetry [11], and spectrophotometry [12,13]. Some of these methods, such as chromatography and spectrophotometry, require a prior separation steps and tedious analytical process during analysis, the use of organic solvents, thereby generating high amounts of waste, time-consuming derivatization steps and high implementation costs, thus justifying the need for reliable, low cost and simpler methods.…”

Utilization of oxidation-reduction reaction of Folin-Ciocalteu’s phenol reagent in colorimetric determination of amlodipine in pharmaceutical dosage form

“…Amlodipine Besylate was given as a gift by Egyptian International Pharmaceutical Industry Co. (EIPICO) (10 th of Ramadan City, Sharqia, Egypt), with a purity of 99.9% according to the USP method [8].…”

“…There are many methods published for determination of AMO in dosage form and in plasma. Most of these methods utilized high-performance liquid chromatography (HPLC) with UV, fluorescence and mass detections [3][4][5][6][7][8][9][10][11][12][13][14]. Some of these methods use spectrofluorometry [15,16].…”

Simple spectrophotometric methods for the simultaneous determination of antipyrine and benzocaine

“…Usually combined dosage forms require more sophisticated analytical methods especially when their components interfere with each other during their analysis and control procedures to resolve the interference. 28 Only two HPLC methods were elaborated for the determination of benzocaine and antipyrine in the presence of BE degradation product. 29,30 One TLC method was reported for the determination of benzocaine and antipyrine in the presence of BE degradation product.…”