New
            <i>lnu</i>
            (C) Gene Conferring Resistance to Lincomycin by Nucleotidylation in
            <i>Streptococcus agalactiae</i>
            UCN36

Achard, Adeline; Villers, Corinne; Pichereau, Vianney; Leclercq, Roland

doi:10.1128/aac.49.7.2716-2719.2005

Cited by 85 publications

(56 citation statements)

References 25 publications

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“…Sequence analysis of the insert identified a gene whose product had 60% identity to LnuC from S. agalactiae (accession number AY928180). LnuC confers lincomycin resistance since it is a lincosamide nucleotidyltransferase that converts the antibiotic to a nontoxic form (5). The high level of sequence identity suggested that the pJIR2774 lincomycin resistance gene in C. perfringens strain 95-948, which we designated lnuP, conferred resistance by a similar mechanism.…”

Section: Resultsmentioning

confidence: 97%

“…tISCpe8, tISSag10 (4,5), and a putative element from Haemophilus parasuis have very similar genetic organizations (Fig. 3), and each element carries only two genes, a gene encoding a putative transposase and a lincomycin resistance gene.…”

Section: Discussionmentioning

confidence: 99%

“…Although cross-resistance to MLS antibiotics most commonly involves N6 dimethylation of the A2058 residue of 23S rRNA and is catalyzed by an erm-encoded rRNA methyltransferase (24,34,47), specific resistance to the lincosamides is the result of modification and inactivation by a lincosamide nucleotidyltransferase encoded by mem-bers of the lnu (previously lin) gene family (5,34,45). This type of resistance gene is found in staphylococci and streptococci, where it is often located on plasmids or transposons (5,45).…”

mentioning

confidence: 99%

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tIS Cpe8 , an IS 1595 -Family Lincomycin Resistance Element Located on a Conjugative Plasmid in Clostridium perfringens

et al. 2009

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Clostridium perfringens is a normal gastrointestinal organism that is a reservoir for antibiotic resistance genes and can potentially act as a source from which mobile elements and their associated resistance determinants can be transferred to other bacterial pathogens. Lincomycin resistance in C. perfringens is common and is usually encoded by erm genes that confer macrolide-lincosamide-streptogramin B resistance. In this study we identified strains that are lincomycin resistant but erythromycin sensitive and showed that the lincomycin resistance determinant was plasmid borne and could be transferred to other C. perfringens isolates by conjugation. The plasmid, pJIR2774, is the first conjugative C. perfringens R-plasmid to be identified that does not confer tetracycline resistance. Further analysis showed that resistance was encoded by the lnuP gene, which encoded a putative lincosamide nucleotidyltransferase and was located on tISCpe8, a functional transposable genetic element that was a member of the IS1595 family of transposon-like insertion sequences. This element had significant similarity to the mobilizable lincomycin resistance element tISSag10 from Streptococcus agalactiae. Like tISSag10, tISCpe8 carries a functional origin of transfer within the resistance gene, allowing the element to be mobilized by the conjugative transposon Tn916. The similarity of these elements and the finding that they both contain an oriT-like region support the hypothesis that conjugation may result in the movement of DNA modules that are not obviously mobile since they are not linked to conjugation or mobilization functions. This process likely plays a significant role in bacterial adaptation and evolution.

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Section: Resultsmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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tIS Cpe8 , an IS 1595 -Family Lincomycin Resistance Element Located on a Conjugative Plasmid in Clostridium perfringens

et al. 2009

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“…It was proposed that MTnSag1 (tISSag10) can be transferred by conjugation and that its acquisition by S. agalactiae might be efficient for the spread of lincomycin resistance since the majority of S. agalactiae strains possess Tn916-like genetic elements (Achard & Leclercq, 2007). However, the insertion of MTnSag1 (tISSag10) in the capsular operon of strain UCN36 has interrupted the cpsE gene; as a consequence the strain was non-typable (Achard et al, 2005). It is thus probable that gain of lincomycin resistance by strain UCN36 is concomitant with a reduction of its virulence.…”

Section: Mobile Genetic Element Of the Is1595 Familymentioning

confidence: 99%

“…The 1724 bp long mobilizable transposon MTnSag1 (also annotated MTnLnu) was found to be inserted within the cpsE gene belonging to the capsular synthesis operon of the S. agalactiae clinical strain UCN36 (Achard et al, 2005). MTnSag1 was reported to encode a transposase related to the InsAB' protein of IS1.…”

Section: Mobile Genetic Element Of the Is1595 Familymentioning

confidence: 99%

Physiological impact of transposable elements encoding DDE transposases in the environmental adaptation of Streptococcus agalactiae

Fléchard

Gilot

2014

Microbiology

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We have referenced and described Streptococcus agalactiae transposable elements encoding DDE transposases. These elements belonged to nine families of insertion sequences (ISs) and to a family of conjugative transposons (TnGBSs). An overview of the physiological impact of the insertion of all these elements is provided. DDE-transposable elements affect S. agalactiae in a number of aspects of its capability to adapt to various environments and modulate the expression of several virulence genes, the scpB-lmB genomic region and the genes involved in capsule expression and haemolysin transport being the targets of several different mobile elements. The referenced mobile elements modify S. agalactiae behaviour by transferring new gene(s) to its genome, by modifying the expression of neighbouring genes at the integration site or by promoting genomic rearrangements. Transposition of some of these elements occurs in vivo, suggesting that by dynamically regulating some adaptation and/or virulence genes, they improve the ability of S. agalactiae to reach different niches within its host and ensure the 'success' of the infectious process.

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Exploring Adenylylation and Phosphocholination as Post‐Translational Modifications

et al. 2013

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New lnu (C) Gene Conferring Resistance to Lincomycin by Nucleotidylation in Streptococcus agalactiae UCN36

Abstract: Streptococcus agalactiae UCN36 was resistant to lincomycin (MIC ‫؍‬ 16 g/ml) but susceptible to clindamycin (MIC ‫؍‬ 0.12 g/ml) and erythromycin (MIC ‫؍‬ 0.06 g/ml).

Cited by 85 publications

References 25 publications

tIS Cpe8 , an IS 1595 -Family Lincomycin Resistance Element Located on a Conjugative Plasmid in Clostridium perfringens

tIS Cpe8 , an IS 1595 -Family Lincomycin Resistance Element Located on a Conjugative Plasmid in Clostridium perfringens

Physiological impact of transposable elements encoding DDE transposases in the environmental adaptation of Streptococcus agalactiae

Exploring Adenylylation and Phosphocholination as Post‐Translational Modifications

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