New Insight into Muscle-Type Cofilin (CFL2) as an Essential Mediator in Promoting Myogenic Differentiation in Cattle

Abstract

Meat quality and meat composition are not separated from the influences of animal genetic improvement systems; the growth and development of skeletal muscle are the primary factors in agricultural meat production and meat quality. Though the muscle-type cofilin (CFL2) gene has a crucial influence on skeletal muscle fibers and other related functions, the epigenetic modification mechanism of the CFL2 gene regulating meat quality remains elusive. After exploring the spatiotemporal expression data of CFL2 gene in a group of samples from fetal bovine, calf, and adult cattle, we found that the level of CFL2 gene in muscle tissues increased obviously with cattle age, whereas DNA methylation levels of CFL2 gene in muscle tissues decreased significantly along with cattle age by BSP and COBRA, although DNA methylation levels and mRNA expression levels basically showed an opposite trend. In cell experiments, we found that bta-miR-183 could suppress primary bovine myoblast differentiation by negatively regulated CFL2. In addition, we packaged recombinant adenovirus vectors for CFL2 gene knockout and overexpression and found that the CFL2 gene could promote the differentiation of primary bovine myoblasts by regulating marker genes MYOD, MYOG and MYH3. Therefore, CFL2 is an essential mediator for promoting myogenic differentiation by regulating myogenic marker genes in cattle myoblasts.

Overexpression of GPX2 gene regulates the development of porcine preadipocytes and skeletal muscle cells through MAPK signaling pathway

“…Recent research has shown that the marker genes MYOD , MYOG , and MYH3 controlled the differentiation of primary myoblasts [ 52 ]. As additional research comes to light, it is becoming increasingly clear that MYOD played a crucial role in myogenic differentiation, which promotes myogenin expression and eases myocyte fusion [ 53 ].…”

Integrative ATAC-seq and RNA-seq Analysis of the Longissimus Dorsi Muscle of Gannan Yak and Jeryak

“…Meanwhile, Liu et al [ 47 ] found that Zbed6 +/− may promote muscle growth in mice by upregulating Barx2 . Additionally, CFL2 , serving as a crucial mediator in promoting bovine myogenic differentiation, facilitates the differentiation of bovine myoblasts through the regulation of muscle differentiation marker gene expression [ 48 ]. The signal visualization revealed a consistent trend in the signals of ATAC-seq and RNA-seq for FOXO1 and CRY2 , both exhibiting robust signals in Jeryak.…”

Comprehensive Analysis of miRNA and mRNA Expression Profiles during Muscle Development of the Longest Dorsal Muscle in Gannan Yaks and Jeryaks

“…In addition, the Six1 gene, a potent regulator of skeletal muscle development [45], activates myogenesis and enhances Myf3 expression by specifically binding to the MEF5 site in the promoter of the gene, thereby promoting skeletal muscle development [46,47]. Sun et al [48]. reported a significant upregulation of CFL2 expression in skeletal muscle, which plays a crucial in regulating the activity of transcription factors involved in muscle formation within C2C12 cells and this, in turn, has an impact on the proliferation and differentiation of myofibroblasts.…”