New insight into the dynamical system of αB-crystallin oligomers

Inoue, Rintaro; Takata, Takumi; Fujii, Norihiko; Ishii, Kentaro; Uchiyama, Susumu; Sato, Nobuhiro; Oba, Yojiro; Wood, Kathleen; Kato, Koichi; Fujii, Nobutaka; Sugiyama, Masaaki

doi:10.1038/srep29208

Cited by 37 publications

(50 citation statements)

References 27 publications

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“…It is well known that the dynamics and polydispersity of oligomers play an important role in the functioning of α-crystallins [ 10 , 29 , 90 , 91 ]. It was reported that subunit exchange promotes structural reorganization within the homo-oligomers of αB-crystallin [ 27 ] (see review [ 29 ]). The equilibrium between oligomeric forms is very sensitive to many factors such as temperature [ 1 , 27 , 92 , 93 , 94 ], post-translational modifications (phosphorylation) [ 33 , 35 , 95 ], divalent cations [ 31 , 32 ], crowding conditions [ 25 ], presence of target protein [ 25 ], and many others.…”

Section: Discussionmentioning

confidence: 99%

“…HSPB5 tends to form large polydisperse assemblies ranging from 10-mer to 40-mer and higher, having very dynamic structures [ 19 , 23 , 24 , 25 ]. All oligomeric forms possess chaperone-like activity and easily exchange their subunits [ 20 , 26 , 27 ]. It is well accepted that a flexible dynamic quaternary structure is necessary for sHSPs activity [ 1 , 4 , 10 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

“…It is well accepted that a flexible dynamic quaternary structure is necessary for sHSPs activity [ 1 , 4 , 10 , 28 ]. Changes in the cellular environment, such as temperature [ 9 , 10 , 27 , 29 ], pH [ 30 ], the presence of ions [ 31 , 32 ], post-translational modification (phosphorylation) [ 33 , 34 , 35 ], redox environment [ 7 , 30 ] and crowding [ 19 , 25 , 32 , 36 , 37 , 38 ] also regulate chaperone activity by affecting the structural and oligomerization dynamics of sHSPs [ 4 , 5 ]. It has been reported that sHSP dynamics is a very complex process that includes five levels of regulation: (1) flexible domains flanking the ACD, (2) polydisperse self-oligomerization, (3) hetero- oligomerization with other sHSPs, (4) subunit exchange, and (5) regulation by the cellular environment [ 5 , 39 ].…”

Section: Introductionmentioning

confidence: 99%

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Chaperone-Like Activity of HSPB5: The Effects of Quaternary Structure Dynamics and Crowding

Chebotareva

Roman

Borzova

et al. 2020

IJMS

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Small heat-shock proteins (sHSPs) are ATP-independent molecular chaperones that interact with partially unfolded proteins, preventing their aberrant aggregation, thereby exhibiting a chaperone-like activity. Dynamics of the quaternary structure plays an important role in the chaperone-like activity of sHSPs. However, relationship between the dynamic structure of sHSPs and their chaperone-like activity remains insufficiently characterized. Many factors (temperature, ions, a target protein, crowding etc.) affect the structure and activity of sHSPs. The least studied is an effect of crowding on sHSPs activity. In this work the chaperone-like activity of HSPB5 was quantitatively characterized by dynamic light scattering using two test systems, namely test systems based on heat-induced aggregation of muscle glycogen phosphorylase b (Phb) at 48 °C and dithiothreitol-induced aggregation of α-lactalbumin at 37 °C. Analytical ultracentrifugation was used to control the oligomeric state of HSPB5 and target proteins. The possible anti-aggregation functioning of suboligomeric forms of HSPB5 is discussed. The effect of crowding on HSPB5 anti-aggregation activity was characterized using Phb as a target protein. The duration of the nucleation stage was shown to decrease with simultaneous increase in the relative rate of aggregation of Phb in the presence of HSPB5 under crowded conditions. Crowding may subtly modulate sHSPs activity.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Chaperone-Like Activity of HSPB5: The Effects of Quaternary Structure Dynamics and Crowding

Chebotareva

Roman

Borzova

et al. 2020

IJMS

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“…In addition, the solution-state conditions allow kinetic, i.e. time-resolved, SANS studies to be carried out, for example on the assembly process of proteasome subunits (Sugiyama et al, 2011), -crystallin oligomerization dynamics (Inoue et al, 2016;Fig. 3a) and the enzyme-mediated unfolding of GFP (Ibrahim et al, 2017;Fig.…”

Section: Introductionmentioning

confidence: 99%

“…Recent examples from the literature illustrating the versatility of Bio-SANS. (a) Models proposed for the -crystallin oligomerization process using a kinetic SANS study of mixtures of hydrogenated and deuterated building blocks(Inoue et al, 2016). (b) Enzyme-mediated unfolding of GFP measured by time-resolved SANS(Ibrahim et al, 2017).…”

mentioning

confidence: 99%

Biological small-angle neutron scattering: recent results and development

Mahieu

Gabel

2018

Acta Cryst Sect D Struct Biol

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Small-angle neutron scattering (SANS) has increasingly been used by the structural biology community in recent years to obtain low-resolution information on solubilized biomacromolecular complexes in solution. In combination with deuterium labelling and solvent-contrast variation (HO/DO exchange), SANS provides unique information on individual components in large heterogeneous complexes that is perfectly complementary to the structural restraints provided by crystallography, nuclear magnetic resonance and electron microscopy. Typical systems studied include multi-protein or protein-DNA/RNA complexes and solubilized membrane proteins. The internal features of these systems are less accessible to the more broadly used small-angle X-ray scattering (SAXS) technique owing to a limited range of intra-complex and solvent electron-density variation. Here, the progress and developments of biological applications of SANS in the past decade are reviewed. The review covers scientific results from selected biological systems, including protein-protein complexes, protein-RNA/DNA complexes and membrane proteins. Moreover, an overview of recent developments in instruments, sample environment, deuterium labelling and software is presented. Finally, the perspectives for biological SANS in the context of integrated structural biology approaches are discussed.

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The Role of Visceral Obesity, Sarcopenia and Sarcopenic Obesity on Surgical Outcomes After Liver Resections for Colorectal Metastases

et al. 2021

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Background The impact of body compositions on surgical results is controversially discussed. This study examined whether visceral obesity, sarcopenia or sarcopenic obesity influence the outcome after hepatic resections of synchronous colorectal liver metastases. Methods Ninety-four consecutive patients with primary hepatic resections of synchronous colorectal metastases were identified from a single center database between January 2013 and August 2018. Patient characteristics and 30-day morbidity were retrospectively analyzed. Body fat and skeletal muscle were calculated by planimetry from single-slice CT images at the level of L3. Results Fifty-nine patients (62.8%) underwent minor hepatectomies, and 35 patients underwent major resections (37.2%). Postoperative complications occurred in 60 patients (62.8%) including 35 patients with major complications (Clavien–Dindo grade III–V). The mortality was nil at 30 days and 2.1% at 90 days. The body mass index showed no influence on postoperative outcomes (p = 1.0). Visceral obesity was found in 66 patients (70.2%) and was significantly associated with overall and major complication rates (p = .002, p = .012, respectively). Sarcopenia was observed in 34 patients (36.2%) without a significant impact on morbidity (p = .461), however, with longer hospital stay. Sarcopenic obesity was found in 18 patients (19.1%) and was significantly associated with postoperative complications (p = .014). Visceral obesity, sarcopenia and sarcopenic obesity were all identified as significant risk factors for overall postoperative complications. Conclusion Visceral obesity, sarcopenic obesity and sarcopenia are independent risk factors for overall complications after resections of CRLM. Early recognition of extremes in body compositions could prompt to perioperative interventions and thus improve postoperative outcomes.

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New insight into the dynamical system of αB-crystallin oligomers

Cited by 37 publications

References 27 publications

Chaperone-Like Activity of HSPB5: The Effects of Quaternary Structure Dynamics and Crowding

Chaperone-Like Activity of HSPB5: The Effects of Quaternary Structure Dynamics and Crowding

Biological small-angle neutron scattering: recent results and development

The Role of Visceral Obesity, Sarcopenia and Sarcopenic Obesity on Surgical Outcomes After Liver Resections for Colorectal Metastases

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