New insight into the mechanisms of Ginkgo biloba leaves in the treatment of cancer

Yu, Jing; Wang, Jinghui; Yang, Jianhua; Ouyang, Ting; Gao, Honglei; Kan, Hongxing; Yang, Yinfeng

doi:10.1016/j.phymed.2023.155088

Cited by 8 publications

(1 citation statement)

References 95 publications

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“…Great attention has been paid to the anti-GC studies based on traditional Chinese medicine (TCM) that are mainly derived from natural products [ 4 ]. These drugs have been reported to show anti-cancer effects through inducing apoptosis and inhibiting the proliferation and metastasis of cancer cells [ 5 ]. One of an important reason for selecting these TCM agents is their abilities to modulate the cancer-related signaling pathways and molecular targets, without causing adverse events [ 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

Gentiopicroside inhibits the progression of gastric cancer through modulating EGFR/PI3K/AKT signaling pathway

Chen,

Zhang,

et al. 2024

Eur J Med Res

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Background This study was designed to clarify the function and potential mechanism of gentiopicroside (GPS) in regulating the malignant progression of gastric cancer (GC) through in vitro cellular experiments and in vivo animal models. Methods AGS and HGC27 cells were divided into control group and GPS treatment groups (50 µM and 100 µM). Then, the cellular proliferation, colony formation, migration, invasion, and apoptosis were detected, respectively. Transmission electron microscope (TEM) was used to observe the mitochondrial changes, and the mitochondrial membrane potential (MMP) was determined using the JC-1 commercial kit. Network pharmacology analysis was utilized to screen the potential molecule that may be related to the GPS activity on GC cells, followed by validation tests using Western blot in the presence of specific activator. In addition, xenografted tumor model was established using BALB/c nude mice via subcutaneous injection of HGC27 cells, along with pulmonary metastasis model. Then, the potential effects of GPS on the tumor growth and metastasis were detected by immunohistochemistry (IHC) and HE staining. Results GPS inhibited the proliferation, invasion and migration of GC cell lines in a dose-dependent manner. Besides, it could induce mitochondrial apoptosis. Epidermal growth factor receptor (EGFR) may be a potential target for GPS action in GC by network pharmacological analysis. GPS inhibits activation of the EGFR/PI3K/AKT axis by reducing EGFR expression. In vivo experiments indicated that GPS induced significant decrease in tumor volume, and it also inhibited the pulmonary metastasis. For the safety concerns, GPS caused no obvious toxicities to the heart, liver, spleen, lung and kidney tissues. IHC staining confirmed GPS downregulated the activity of EGFR/PI3K/AKT. Conclusions Our investigation demonstrated for the first time that GPS could inhibit GC malignant progression by targeting the EGFR/PI3K/AKT signaling pathway. This study indicated that GPS may be serve as a safe anti-tumor drug for further treatment of GC.

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Section: Introductionmentioning

confidence: 99%

Gentiopicroside inhibits the progression of gastric cancer through modulating EGFR/PI3K/AKT signaling pathway

Chen,

Zhang,

et al. 2024

Eur J Med Res

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An effective and comprehensive optimization strategy for preparing Ginkgo biloba leaf extract enriched in shikimic acid by macroporous resin column chromatography

Zhang,

Gong,

2024

Phytochemical Analysis

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IntroductionPreviously reported preparation methods of Ginkgo biloba leaf extract (EGBL) have mainly focused on the enrichment of flavonoid glycosides (FG) and terpene trilactones (TT), which led to the underutilization of G. biloba leaves (GBL).ObjectivesTo make full use of GBL, in this study, a comprehensive optimization strategy for preparing EGBL by macroporous resin column chromatography was proposed and applied to enrich FG, TT, and shikimic acid (SA) from GBL.MethodologyInitially, the static adsorption and desorption were executed to select suitable resin. Then, the influences of solution pH were investigated by the static and dynamic adsorption. Subsequently, eight process parameters were systematically investigated via a definitive screening design (DSD). After verification experiments, scale‐up enrichment was carried out, investigating the feasibility of the developed strategy for application on an industrial scale.ResultsIt was found that XDA1 was the most appropriate adsorbent for the preparation of EGBL at solution pH 2.0. Furthermore, based on the constraints of the desired quality attributes, the optimized ranges of operating parameters were successfully acquired, and the verification experiments demonstrated the accuracy and reliability of using DSD to investigate the chromatography process for the preparation of EGBL. Finally, magnified experiments were successfully performed, obtaining the EGBL containing 26.54% FG, 8.96% TT, and 10.70% SA, which reached the SA level of EGB761, an international standard EGBL.ConclusionThe present study not only provided an efficient and convenient approach for the preparation of EGBL enriched in SA but also accelerated efforts to high‐value utilization of GBL.

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Molecular mechanisms of apoptosis induction in KG1a leukemia cells by the Ginkgo biloba extract (EGb)

Babaei,

Abdzadeh,

Bakhshandeh

et al. 2024

ADV TRADIT MED (ADTM)

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New insight into the mechanisms of Ginkgo biloba leaves in the treatment of cancer

Cited by 8 publications

References 95 publications

Gentiopicroside inhibits the progression of gastric cancer through modulating EGFR/PI3K/AKT signaling pathway

Gentiopicroside inhibits the progression of gastric cancer through modulating EGFR/PI3K/AKT signaling pathway

An effective and comprehensive optimization strategy for preparing Ginkgo biloba leaf extract enriched in shikimic acid by macroporous resin column chromatography

Molecular mechanisms of apoptosis induction in KG1a leukemia cells by the Ginkgo biloba extract (EGb)

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