New insight into the molecular basis of chromium exposure of Litopenaeus vannamei by transcriptome analysis

Dai, Tianmeng; Cao, Tinglan; Jin, Min; Sun, Peng; Zhou, Qicun

doi:10.1016/j.marpolbul.2020.111673

Cited by 18 publications

(7 citation statements)

References 54 publications

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“…Lysosomes are also essential to crustaceans’ innate immune system, providing protection against pathogens (Guo et al, 2019, 2021; Yu et al, 2018). In addition, lysosomal pathways and associated enzymes are sensitive to various environmental stressors (Guo et al, 2021; Jiao et al, 2020; Yu et al, 2018), as highlighted in the present study which showed that the lysosomal enzyme transcripts cathepsin L and legumain lowered in abundance after 24 h of exposure to oil‐contaminated sediments. Similarly, the lysosome pathway in Chinese mitten crabs and the phagosome pathway in green mussel embryos was altered following exposure to BaP (Jiang et al, 2016; Yu et al, 2018).…”

Section: Discussionsupporting

confidence: 66%

“…Similarly, the lysosome pathway in Chinese mitten crabs and the phagosome pathway in green mussel embryos was altered following exposure to BaP (Jiang et al, 2016; Yu et al, 2018). In addition, cathepsin L was also lowered in expression after exposure to BaP in green mussels (Jiang et al, 2016) and to chromium in Pacific white shrimp (Jiao et al, 2020). Conversely, higher expression of cathepsin L was found in decapods after exposure to cadmium (Sun et al, 2016) and ammonia (Yu et al, 2019) when compared to the control.…”

Section: Discussionmentioning

confidence: 99%

“…The “base excision repair” pathway is of particular interest as it is the primary repair pathway utilized for small base lesions from oxidation and alkylation damage caused by a range of endogenous and exogeneous sources (Almeida & Sobol, 2007). The “base excision repair” pathway was significantly enriched in freshwater crabs exposed to cadmium and in Pacific white shrimp exposed to chromium (Jiao et al, 2020; Sun et al, 2016). However, after prawns were transferred to clean sediment tanks for a 72‐h recovery period a reversal in genetic information processing and cellular processes pathways was observed.…”

Section: Discussionmentioning

confidence: 99%

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Transcriptomic and Histological Analysis of the Greentail Prawn (Metapenaeus bennettae) Following Light Crude Oil Exposure

Armstrong

Mondon

Miller

et al. 2022

Enviro Toxic and Chemistry

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Oil spills pose a significant threat to marine biodiversity. Crude oil can partition into sediments where it may be persistent, placing benthic species such as decapods at particular risk of exposure. Transcriptomic and histological tools are often used to investigate the effects of hydrocarbon exposure on marine organisms following oil spill events, allowing for the identification of metabolic pathways impacted by oil exposure. However, there is limited information available for decapod crustaceans, many of which carry significant economic value. In the present study, we assess the sublethal impacts of crude oil exposure in the commercially important Australian greentail prawn (Metapenaeus bennettae) using transcriptomic and histological analyses. Prawns exposed to light, unweathered crude oil "spiked" sediments for 90 h were transferred to clean sediments for a further 72 h to assess recovery. Chemical analyses indicated that polycyclic aromatic hydrocarbons increased by approximately 65% and 91% in prawn muscle following 24 and 90 h of exposure, respectively, and significantly decreased during 24-and 72-h recovery periods. Transcriptomic responses followed an exposure and recovery pattern with innate immunity and nutrient metabolism transcripts significantly lowered in abundance after 24 h of exposure and were higher in abundance after 72 h of recovery. In addition, transcription/translation, cellular responses, and DNA repair pathways were significantly impacted after 24 h of exposure and recovered after 72 h of recovery. However, histological alterations such as tubule atrophy indicated an increase in severity after 24 and 72 h of recovery. The present study provides new insights into the sublethal impacts of crude oil exposure in greentail prawns and identifies molecular pathways altered by exposure. We expect these findings to inform future management associated with oil extraction activity and spills.

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Section: Discussionsupporting

confidence: 66%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Transcriptomic and Histological Analysis of the Greentail Prawn (Metapenaeus bennettae) Following Light Crude Oil Exposure

Armstrong

Mondon

Miller

et al. 2022

Enviro Toxic and Chemistry

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“…Total hepatopancreas RNA was extracted from tissues using TRIzol reagents (Invitrogen, Waltham, MA, United States) and then determined its quality and quantity as previously described (Jiao et al, 2020). Subsequently, mRNA was isolated and fragmented for cDNA synthesis.…”

Section: Hepatopancreas Transcriptome Analysismentioning

confidence: 99%

“…The Pacific white shrimp Litopenaeus vannamei is considered a paramount aquaculture species, accounting for more than 70% of the total global shrimp production worldwide (Jiao et al, 2020). L. vannamei starts benthic life soon after the mysis stage or the early stage of postlarvae.…”

Section: Introductionmentioning

confidence: 99%

Influence of Light/Dark Cycles on Body Color, Hepatopancreas Metabolism, and Intestinal Microbiota Homeostasis in Litopenaeus vannamei

Dai

Tao

et al. 2021

Front. Mar. Sci.

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In aquatic animals, the light/dark cycle acts as an important biological factor that influences the entire life cycle. Until present, evidence regarding the regulation of physiological metabolic process under different light/dark cycles is limited in Litopenaeus vannamei. In this study, we mainly investigated the effects of different light/dark cycles (12 h light/12 h dark, 0 h light/24 h dark) on the hepatopancreas metabolism and intestinal microbiota homeostasis in L. vannamei using multiomics techniques. One interesting finding was that the body color of L. vannamei became darker after dark treatment for 8 weeks. Further hepatopancreas transcriptome analysis identified down-regulated genes involved in regulating nutrition metabolism, body-color formation, diurnal rhythm, immune function, hormone levels, and posttranslational modifications. The intestinal microbiota analysis showed that dark treatment-induced alterations in intestinal bacterial abundances in L. vannamei, such as decreased (P < 0.05) relative abundance of Formosa, Demequina, Lutimonas and increased (P < 0.05) relative abundance of Ruegeria, Vibrio, Actibacter, Roseovarius, Ilumatobacter, and Kriegella at the genus level. The microbiota functional analysis demonstrated that the dark treatment mainly increased susceptibility of pathogens, decreased nutrition metabolism, and influenced circadian rhythm. This study indicated for the first time that constant darkness treatment darkened the body color and altered hepatopancreas metabolism and intestinal microbiota homeostasis in L. vannamei, which might give potential clues for improving the productive capacities by changing light/dark cycles in shrimp farming.

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Acute environmental hypoxia induced changes of tissue morphology, antioxidant capacity, immune response and energy metabolism in Marsupenaeus japonicus

Feng

Zhang

Chen

et al. 2023

Aquaculture Reports

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New insight into the molecular basis of chromium exposure of Litopenaeus vannamei by transcriptome analysis

Cited by 18 publications

References 54 publications

Transcriptomic and Histological Analysis of the Greentail Prawn (Metapenaeus bennettae) Following Light Crude Oil Exposure

Transcriptomic and Histological Analysis of the Greentail Prawn (Metapenaeus bennettae) Following Light Crude Oil Exposure

Influence of Light/Dark Cycles on Body Color, Hepatopancreas Metabolism, and Intestinal Microbiota Homeostasis in Litopenaeus vannamei

Acute environmental hypoxia induced changes of tissue morphology, antioxidant capacity, immune response and energy metabolism in Marsupenaeus japonicus

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