New Insight on Antibiotic Resistance and Virulence of Escherichia coli from Municipal and Animal Wastewater

Gregová, G.; Kmet, V.; Szabóová, Tatiana

doi:10.3390/antibiotics10091111

Cited by 11 publications

(6 citation statements)

References 43 publications

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“…Escherichia coli, which also causes infections in humans, is present in water treatment plants, in farm animal and wildlife waste, and has been associated with MDR (Gregova et al, 2021;Savin et al, 2021). Gregova et al (2021), studied the virulence of E. coli in two municipal water treatment plants and water used to treat animals in Slovakia and described the resistance of this isolate to AMP (68% and 46%), CLO (32% and 11.4%) respectively in the two types of water and ERT (55%) for the two waters examined, in addition to R for other ATBs not performed in our study such as: nadixic acid (68% and 46%), clotrimazole (63% and 37%) and orfenicol (36.5% and 16%) respectively for municipal waters and animal treatment waters and teracyclic (61%) for both waters.…”

Section: Discussionmentioning

confidence: 99%

Bovine colostrum and multidrug resistance: One Health Vision for human consumption

Jara,

Cardozo,

Kraus

et al. 2024

Preprint

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One Health represents a holistic approach to understanding the interconnectedness of the human-animal-environment triad in the context of achieving health and well-being. In this context, surplus bovine colostrum (BC) not consumed by calves is important due to its potential nutritional value for human consumption. However, it is imperative to ensure their safety and quality to reach the market. It is important to reflect on the presence of pathogenic microorganisms and their multi-resistance to antimicrobials for use in animals and humans, which can have serious consequences for One Health. The objective here was to verify the susceptibility profile of bacteria found in BC and bovine colostrum silage to antibiotics. The strains studied cover the genera Escherichia spp., Klebsiella sp., Enterococcus sp., Staphylococcus spp. and Streptococcus sp.. Susceptibility tests were carried out using the Kirb & Bauer method following guidelines from BrCAST and CLSI (2023). Antibiotics evaluated included quinolones, penicillin, cephalosporins, carbapenems, β-lactamase inhibitors, polypeptides, aminoglycosides, glycopeptides, macrolides, oxalidinones, lincosamines, rifampins, and phenicols. Although the results indicate low overall resistance, the study highlights the presence of multi-resistant bacteria in raw BC and BC silage, highlighting the risk of transmission of these and their resistance genes to humans if rigorous processing measures are not carried out to mitigate the One Health risks.

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Section: Discussionmentioning

confidence: 99%

Bovine colostrum and multidrug resistance: One Health Vision for human consumption

Jara,

Cardozo,

Kraus

et al. 2024

Preprint

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“…It causes bacterial intestinal and extra-intestinal infections including septicaemia, diarrhoea, urinary tract infections, and neonatal meningitis in man and livestock ( 24 ). It is often linked with multiple antimicrobial resistances ( 25 ), and among bacteria isolated from animal foods, such as pigs, cattle, and poultry ( 26 ).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the physicochemical properties and bacterial loads of selected rivers in Ondo State, Nigeria

Bello,

Akinpeloye,

Bello

et al. 2023

IJM

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Background and Objectives: Water is crucial to human existence but may be contaminated with microorganisms, thus making it unfit for consumption. This study aimed to evaluate the physicochemical properties and bacterial loads of selected river waters in Ondo State, Nigeria. Materials and Methods: Ten major rivers were sampled between April and August, 2021. The pH, temperature, total al- kalinity, colour, turbidity, electrical conductivity, dissolved oxygen, ammonium, aluminium, organic matters, nitrate levels including the microbial loads were determined according to standard procedures. Confirmation of identified isolates was achieved by API 20E and API 20NE. Results: The turbidity, colour, conductivity, ammonium, and aluminium ranged from 4.3 to 15.2 NTU, 4 to 20 NTU, 123.5 to 580.2 mgL-1, 0 to 3.5 mgL-1, and 0.05 to 1.7, respectively. Most physicochemical parameters showed no significant differences from the WHO permissible limits for drinking water (p > 0.05). The total viable bacterial count in the rivers ranged from 1.5 × 105 to 6.3 × 105 CFUmL-1, while the total coliform count ranged from 1.3 × 103 to 4.8 × 103 CFUmL-1. The predominant bacteria were Escherichia coli and Pseudomonas aeruginosa. Conclusion: This study revealed that the physiochemical properties of the waters were majorly within the WHO permissible standards but with significantly higher bacterial loads.

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“…The bla CTX-M (group 1, 2, and 9) genes were detected via M-PCRs ( Dallenne et al, 2010 ), and the bla TEM and bla SHV genes were detected using a simplex PCR ( Pitout et al, 1998 ). The presence of integrons ( intI1 and int2 ) ( Kurekci et al, 2017 ), transposon (Tn 3 ) ( Gregova, Kmet & Szaboova, 2021 ), and insertion sequence (IS Ecp1 ) ( Eckert, Gautier & Arlet, 2006 ) were detected by PCR as described previously. DNA sequencing of PCR products (CTX-M1, M9, and TEM) was used.…”

Section: Methodsmentioning

confidence: 99%

Detection and characterization of ESBL-producing Escherichia coli and additional co-existence with mcr genes from river water in northern Thailand

Chotinantakul

Chusri

Okada

2022

PeerJ

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Background Extended-spectrum β-lactamase producing Escherichia coli (ESBL-producing E. coli) have emerged, causing human and animal infections worldwide. This study was conducted to investigate the prevalence and molecular genetic features of ESBL-producing and multidrug-resistant (MDR) E. coli in river water. Methods A total of 172 E. coli samples were collected from the Kok River and Kham River in Chiang Rai, Thailand, during a 10-month period (2020–2021). Results We detected 45.3% of E. coli to be MDR. The prevalence of ESBL-producers was 22%. Among those ESBL-producing strains, CTX-M-15 (44.7%) was predominantly found, followed by CTX-M-55 (26.3%), CTX-M-14 (18.4%), and CTX-M-27 (10.5%). The blaTEM-1 and blaTEM-116 genes were found to be co-harbored with the blaCTX-M genes. Mobile elements, i.e., ISEcp1 and Tn3, were observed. Twelve plasmid replicons were found, predominantly being IncF (76.3%) and IncFIB (52.6%). Whole genome sequencing of ten selected isolates revealed the co-existence of ESBL with mcr genes in two ESBL-producing E. coli. A wide diversity of MLST classifications was observed. An mcr-1.1-pap2 gene cassette was found to disrupt the PUF2806 domain-containing gene, while an mcr-3.4 contig on another isolate contained the nimC/nimA-mcr-3.4-dgkA core segment. Discussion In conclusion, our data provides compelling evidence of MDR and ESBL-producing E. coli, co-existing with mcr genes in river water in northern Thailand, which may be disseminated into other environments and so cause increased risks to public health.

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New Insight on Antibiotic Resistance and Virulence of Escherichia coli from Municipal and Animal Wastewater

Cited by 11 publications

References 43 publications

Bovine colostrum and multidrug resistance: One Health Vision for human consumption

Bovine colostrum and multidrug resistance: One Health Vision for human consumption

Evaluation of the physicochemical properties and bacterial loads of selected rivers in Ondo State, Nigeria

Detection and characterization of ESBL-producing Escherichia coli and additional co-existence with mcr genes from river water in northern Thailand

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