New insights into molecular pathways associated with flatfish ovarian development and atresia revealed by transcriptional analysis

Tingaud‐Sequeira, Angèle; Chauvigné, François; Lozano, Juan José; Agulleiro, Maria Josep; Asensio, Esther; Cerdà, Joan

doi:10.1186/1471-2164-10-434

Cited by 59 publications

(52 citation statements)

References 106 publications

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“…Several studies have dealt with qPCR normalization in fish (Aursnes et al, 2011;Bower and Johnston, 2009;Fernandez et al, 2008;Infante et al, 2008;Jorgensen et al, 2005;Øvergard et al, 2010). And while there have been also studies directed at the differential expression of genes during oocyte development in teleosts (Luckenbach et al, 2008a(Luckenbach et al, , 2008bTingaud-Sequeira et al, 2009;Von Schalburg et al, 2005), this is the first study which characterized genes with stable expression throughout oocyte growth to be used as normalizers.…”

Section: Pattern Of Expression Levels Of Reference Genesmentioning

confidence: 96%

Reference genes to quantify gene expression during oogenesis in a teleost fish

Deloffre

Andrade

Filipe

et al. 2012

Gene

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Section: Pattern Of Expression Levels Of Reference Genesmentioning

confidence: 96%

Reference genes to quantify gene expression during oogenesis in a teleost fish

Deloffre

Andrade

Filipe

et al. 2012

Gene

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“…The mitochondria reportedly have anti-apoptotic and antioxidant functions in germ cells and granulosa cells (28,29). The mitochondrial regulators, NRF-1 and PGC-1α, interact to regulate oxidative metabolism (30,31).…”

Section: Discussionmentioning

confidence: 99%

Using cysteine/cystine to overcome oxidative stress in goat oocytes and embryos cultured in vitro

Zhou

Jia

Zhang

et al. 2016

Molecular Medicine Reports

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Abstract. Assisted reproductive techniques expose gametes to excessive concentrations of reactive oxygen species. The present study aimed to evaluate the effects of oxidative stress on apoptosis in goat oocytes and embryonic development. The results demonstrated that the addition of 100 µM hydrogen peroxide (H 2 O 2 ) into media produces an oxidative environment during oocyte maturation. The number of cumulus cells positive for terminal deoxynucleotidyl transferase UTP nick end labeling, and the activity of caspase 3 in mature oocytes were increased, compared with the control group (P<0.05). In addition, the expression levels of mitochondrial regulators, including peroxisome proliferator-activated receptor γ coactivator-1 α (PGC-1α) and nuclear respiratory factor-1 (NRF-1) were increased in the oxidative oocytes, compared with those in the control group (P<0.05). The ratio of the proapoptotic gene, B cell lymphoma (Bcl-2)-associated X pr otein (BAX), to the anti-apoptotic gene, BCL-2, was higher in the H 2 O 2 group, compared with the control group (P<0.05). To overcome oxidative stress in oocytes and embryos cultured in vitro, 200 µM cysteine and 200 µM cystine were added to the media, thereby increasing the concentration of intracellular glutathione (GSH) and assisting in maintaining the redox state of the cells. In conclusion, cysteine and cystine reduced the oxygen tension caused by H 2 O 2 , thereby providing a novel strategy for optimizing in vitro embryonic development systems.

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“…7). The Because Senegalese sole exhibits group-synchronous ovarian development [68], a sample of ovarian tissue from each female collected was fixed for histological determination of the specific stage of ovarian development. As shown in Figure 7F, ovaries collected in July and January retained exclusively previtellogenic follicles at the primary growth stage (Fig.…”

Section: Expression Of Sole Fshra and Lhcgrba In The Ovary During Oocmentioning

confidence: 99%

Functional and Evolutionary Analysis of Flatfish Gonadotropin Receptors Reveals Cladal- and Lineage-Level Divergence of the Teleost Glycoprotein Receptor Family1

Chauvigné

Tingaud‐Sequeira

Agulleiro

et al. 2010

Biology of Reproduction

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Pituitary gonadotropins, follicle-stimulating hormone (FSH), and luteinizing hormone (LH) act via their cognate glycoprotein hormone receptors (GpHRs), FSH receptor (FSHR), and LH/choriogonadotropin receptor (LHCGR) to regulate gonad physiology. Here, we show that the flatfish Senegalese sole (Solea senegalensis) expresses functional isoforms of fshr and lhcgr, but the genomic origin, ligand activation, and tissue distribution of the receptor transcripts are more complex than expected. By integrating the molecular phylogeny of GpHRs with the syntenic loci of vertebrate orthologs, and by subsequently characterizing the physical maps with the phylogeny of flanking genes, we found that vertebrate GpHRs have undergone a divergent evolution. In Teleostei, fshr genes have a common descent and can be classified as fshra, whereas lhcgrb genes exist as alternatively coded genes even in closely related species. Structural analyses of the receptors revealed that Fshra has an elongated ligand-binding domain, containing an extra leucine-rich repeat that specifically arose in the Acanthomorpha because of exon duplication. Ectopic expression in Xenopus laevis oocytes demonstrated that sole Fshra responded to piscine Fsh and Lh, whereas Lhcgrba was preferentially activated by its cognate hormone. The expression pattern of sole fshra and lhcgrba in gonads during the reproductive cycle was consistent with earlier observations wherein Fshra regulates ovarian growth and spermatogenesis and Lhcgrb triggers gamete maturation, respectively. However, contrary to observations in other teleosts, fshra was localized exclusively in Sertoli cells of the testis, whereas lhcgrba was expressed in Leydig cells as well as in spermatids. These results demonstrate the presence of alternatively coded lhcgr isoforms (lhcgrba and lhcgrbb) in teleosts and suggest a role of the lhcgrba receptor in the differentiation of spermatids into spermatozoa in Senegalese sole.

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New insights into molecular pathways associated with flatfish ovarian development and atresia revealed by transcriptional analysis

Cited by 59 publications

References 106 publications

Reference genes to quantify gene expression during oogenesis in a teleost fish

Reference genes to quantify gene expression during oogenesis in a teleost fish

Using cysteine/cystine to overcome oxidative stress in goat oocytes and embryos cultured in vitro

Functional and Evolutionary Analysis of Flatfish Gonadotropin Receptors Reveals Cladal- and Lineage-Level Divergence of the Teleost Glycoprotein Receptor Family1

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