New insights into polyurethane biodegradation and realistic prospects for the development of a sustainable waste recycling process

Cregut, Mickaël; Bedas, Marion; Durand, Marie-José; Thouand, Gérald

doi:10.1016/j.biotechadv.2013.08.011

Cited by 216 publications

(139 citation statements)

References 48 publications

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“…Impranil colloids were added to cell-free supernatants of Pf-5 and pueA or pueB mutants (Pf5⌬pueA, Pf5⌬pueB, and Pf5⌬pueAB) grown in M9-citrate medium. After 24 h of incubation, clarified samples were analyzed by 1 H NMR for the presence of hydrolysis products. (C) Cell-free supernatants of M9-citrate cultures were also incubated with 4-NPP to assess general lipase activities of the strains.…”

Section: Discussionmentioning

confidence: 99%

Carbon Catabolite Repression and Impranil Polyurethane Degradation in Pseudomonas protegens Strain Pf-5

Hung

Zingarelli

Nadeau

et al. 2016

Appl Environ Microbiol

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Polyester polyurethane (PU) coatings are widely used to help protect underlying structural surfaces but are susceptible to biological degradation. PUs are susceptible to degradation by Pseudomonas species, due in part to the degradative activity of secreted hydrolytic enzymes. Microorganisms often respond to environmental cues by secreting enzymes or secondary metabolites to benefit their survival. This study investigated the impact of exposing several Pseudomonas strains to select carbon sources on the degradation of the colloidal polyester polyurethane Impranil DLN (Impranil). The prototypic Pseudomonas protegens strain Pf-5 exhibited Impranil-degrading activities when grown in sodium citrate but not in glucose-containing medium. Glucose also inhibited the induction of Impranil-degrading activity by citrate-fed Pf-5 in a dose-dependent manner. Biochemical and mutational analyses identified two extracellular lipases present in the Pf-5 culture supernatant (PueA and PueB) that were involved in degradation of Impranil. Deletion of the pueA gene reduced Impranil-clearing activities, while pueB deletion exhibited little effect. Removal of both genes was necessary to stop degradation of the polyurethane. Bioinformatic analysis showed that putative Cbr/Hfq/Crc-mediated regulatory elements were present in the intergenic sequences upstream of both pueA and pueB genes. Our results confirmed that both PueA and PueB extracellular enzymes act in concert to degrade Impranil. Furthermore, our data showed that carbon sources in the growth medium directly affected the levels of Impranil-degrading activity but that carbon source effects varied among Pseudomonas strains. This study uncovered an intricate and complicated regulation of P. protegens PU degradation activity controlled by carbon catabolite repression. IMPORTANCEPolyurethane (PU) coatings are commonly used to protect metals from corrosion. Microbiologically induced PU degradation might pose a substantial problem for the integrity of these coatings. Microorganisms from diverse genera, including pseudomonads, possess the ability to degrade PUs via various means. This work identified two extracellular lipases, PueA and PueB, secreted by P. protegens strain Pf-5, to be responsible for the degradation of a colloidal polyester PU, Impranil. This study also revealed that the expression of the degradative activity by strain Pf-5 is controlled by glucose carbon catabolite repression. Furthermore, this study showed that the Impranil-degrading activity of many other Pseudomonas strains could be influenced by different carbon sources. This work shed light on the carbon source regulation of PU degradation activity among pseudomonads and identified the polyurethane lipases in P. protegens.

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Section: Discussionmentioning

confidence: 99%

Carbon Catabolite Repression and Impranil Polyurethane Degradation in Pseudomonas protegens Strain Pf-5

Hung

Zingarelli

Nadeau

et al. 2016

Appl Environ Microbiol

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“…Polyurethanes account for 6e7% of the total mass of plastics generated all over the world and over half of the total global market for automotive and commercial coating applications [1,2]. As a result, the waste generated from the raw materials and the degradation of the polyurethanes is of significant interest to the global community.…”

Section: Introductionmentioning

confidence: 99%

The applicability of Impranil®DLN for gauging the biodegradation of polyurethanes

Biffinger

Barlow

Cockrell

et al. 2015

Polymer Degradation and Stability

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“…Esto dejaría libres los grupos carbonilo del enlace uretano aumentando la señal en 1731 cm -1 como se puede ver en la figura. 31 Algunos reportes indican que el poliuretano de tipo poliéster es susceptible a la biodegradación en los enlaces éster y que el de tipo poliéter es degradado química o físicamente debido a sus enlaces más estables, 32 así que si la biodegradación de este tipo de poliuretano rígido ocurre, está podría iniciarse en los enlaces uretano, como algunos reportes 4,33 4-dicarbamic acid dibutyl ester (TDCB lo indican.…”

Section: 29unclassified

Fungal biodegradation of rigid polyurethane

et al. 2017

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FUNGAL BIODEGRADATION OF RIGID POLYURETHANE. Polyurethane (PU) is one of the most widely used polymer materials. This generates a serious environmental problem because of its poor biodegradability since most of the native microorganisms cannot use it as a source of nutrients. Several techniques involving mechanical and chemical processes have been used for its recovery and reuse; however, the problem of contamination has not been solved, and recently research has focused on developing strategies that use different microorganisms for the biodegradation of PU. In this work, the biodegradation and enzymatic capacity of three fungal strains were evaluated. Thermogravimetric and infrared spectrophotometry analyzes were used to evaluate the ability of the three fungal strains to biodegrade PU and also their enzymatic activities were studied. It was found that a strain of Trichoderma was able to modify the PU structure enough that the changes were detected by both TGA and FTIR. These changes can be caused by urease activity that breaks down carbon-nitrogen bonds in the structure because the signals suggesting the formation of carboxylic acids and decreasing corresponding to carbon-nitrogen bonds of the urethane bond are increased.Keywords: polyurethane; biodegradation; filamentous fungi. INTRODUCCIONEn el mundo, los polímeros son los principales responsables del deterioro del medio ambiente.1 Dentro de estos, los poliuretanos (PU) son ampliamente usados como materia prima en diversas industrias, desde muebles y automóviles, a aislamientos térmicos de barcos y materiales médicos.2 Son sintetizados a partir de polioles y poliisocianatos y se caracterizan por ser insolubles en agua.3 El problema principal radica en su acumulación y no biodegradación por parte de los microorganismos existentes, que no cuentan con estrategias de biodegradación contra esta estructura polimérica. 4 Cada año, más de 5 millones de toneladas de residuos que contienen diferentes plásticos y espumas de PU son generados en los Estados Unidos y Canadá, 3 por lo que la degradación del PU y el descubrimiento de microorganismos capaces de aprovecharlo como fuerte de carbono o nitrógeno, se ha convertido en un pilar dentro de la investigación científica hacia una química más verde. 5Los polímeros pueden ser sustratos potenciales para microorganismos quimioheterotrofos.6 Se han aislado tanto bacterias como hongos filamentosos capaces de degradar PU.7 Los géneros de hongos encontrados han sido Geomyces pannorum y Nectria sp, principalmente, además de Cylindrocladiella parva, Penicillium inflatum, Plectosphaerella cucumerin y P. inflatum, Alternaria, Penicillium venetum, Neonectria ramulariae y Penicillium viridicatum. Los poliurteanos están conformados por regiones C-C, C-N y C-O y poseen un enlace característico, el enlace uretano, similar al enlace peptídico y a la urea. La principal funcion del enlace uretano es unir cadenas orgánicas de polioles y poliisocianatos, para formar la unidad polimérica del poliuretano, la cual diferencia a cada poliuretano ...

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New insights into polyurethane biodegradation and realistic prospects for the development of a sustainable waste recycling process

Cited by 216 publications

References 48 publications

Carbon Catabolite Repression and Impranil Polyurethane Degradation in Pseudomonas protegens Strain Pf-5

Carbon Catabolite Repression and Impranil Polyurethane Degradation in Pseudomonas protegens Strain Pf-5

The applicability of Impranil®DLN for gauging the biodegradation of polyurethanes

Fungal biodegradation of rigid polyurethane

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