New insights into the biosynthesis of esterified oxylipins and their involvement in plant defense and developmental mechanisms

Genva, Manon; Akong, Firmin Obounou; Andersson, Mats X.; Deleu, Magali; Lins, Laurence; Fauconnier, Marie‐Laure

doi:10.1007/s11101-018-9595-8

Cited by 49 publications

(37 citation statements)

References 100 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Certain undesired microbe interaction with plant host produced hypersensitive response which can be determined by the production of hypersensitive inducing molecules in plants (Terrón-Camero et al, 2018). We quantified two intracellular signalling molecules for inducing HR (c-di-GMP (Terrón-Camero et al, 2018) and cAMP level (Almblad et al, 2019) and four metabolites (phosphatidic acid (PA) pure Oxo-phytodeinoic acid (OPDA), esterified OPDA, and Jasmonic acid (JA) in maize seedlings through GC/MS technique (Genva et al, 2019). Result deducted that under uniconizole treatment, the concentration of these hypersensitive molecules was same as control (Figure 5A-5J & 6A-6T).…”

Section: Resultsmentioning

confidence: 99%

Phytohormonal cross-talk modulate Bipolaris sorokiniana (Scc.)interaction with Zea mays

Yousaf

Hussain

Hamayun

et al. 2019

Preprint

View full text Add to dashboard Cite

16Besides acting as growth inducing molecule, Gibberellin (GA 3 ) also confers the compatibility 17 of microbial interactions with host. We inoculated 11 days old Z. mays seedlings grown under 18 hydroponic conditions and high GA 3 levels with Bipolaris sorokiniana (BIPOL) at the spore 19 density (SD) of OD 0.6 . The high level of GA 3 negatively affected the growth of the seedlings, 20 accompanied by the high level of stress deducing secondary metabolites (proline, total 21 flavanoids, phenylpropanoids, and glucosinolides). Moreover, high level of GA 3 produced a 22 hypersensitive response (HR) in the seedlings. The HR developed cross talks with IAA and 23 trans-zeatins and triggered higher production of hypersensitive inducing biomolecules. The 24 other HR co-related biological processes were demonstrated by high phytoalexins level and 25 high protease activities. Such activities ultimately inhibited the colonization of BIPOL on the 26 roots of maize seedlings. The products of the genes expressed at high GA 3 also conferred the 27 deterrence of BIPOL colonization at SD = OD 0.6 . Intriguingly, when we inhibited GA 3 28 biosynthesis in the seedlings with aerially sprayed uniconizole, prior to BIPOL treatment, the 29 BIPOL colonized and subsequently promoted the seedling growth. This low level of GA 3 30 after BIPOL treatment checked the high level of secondary metabolites and hypersensitivity 31 inducing molecules. The results, thus suggested that the aforementioned processes only 32 happened in the BIPOL at SD (OD 0.6 ), whereas the SD at lower levels (OD 0.2 or OD 0.4 ) 33 neither promoted the growth of uniconizole pre-treated seedlings nor produced HR in control 34 seedlings of maize plant.35

show abstract

Section: Resultsmentioning

confidence: 99%

Phytohormonal cross-talk modulate Bipolaris sorokiniana (Scc.)interaction with Zea mays

Yousaf

Hussain

Hamayun

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…Of note, OPDA is also found esterified to galactolipids (8,9). However, it has not been entirely clarified whether esterified oxylipins are produced from free fatty acid intermediates or whether synthesis can occur on fatty acids while they are esterified into lipids (10).…”

mentioning

confidence: 99%

JASSY, a chloroplast outer membrane protein required for jasmonate biosynthesis

Guan

Denkert

Eisa

et al. 2019

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Jasmonates are vital plant hormones that not only act in the stress response to biotic and abiotic influences, such as wounding, pathogen attack, and cold acclimation, but also drive developmental processes in cooperation with other plant hormones. The biogenesis of jasmonates starts in the chloroplast, where several enzymatic steps produce the jasmonate precursor 12-oxophytodienoic acid (OPDA) from α-linolenic acid. OPDA in turn is exported into the cytosol for further conversion into active jasmonates, which subsequently induces the expression of multiple genes in the nucleus. Despite its obvious importance, the export of OPDA across the chloroplast membranes has remained elusive. In this study, we characterized a protein residing in the chloroplast outer membrane, JASSY, which has proven indispensable for the export of OPDA from the chloroplast. We provide evidence that JASSY has channel-like properties and propose that it thereby facilitates OPDA transport. Consequently, a lack of JASSY in Arabidopsis leads to a deficiency in accumulation of jasmonic acids, which results in impaired expression of jasmonate target genes on exposure to various stresses. This results in plants that are more susceptible to pathogen attack and also exhibit defects in cold acclimation. jasmonate | plant hormones | chloroplast | membrane pore | cold acclimation Author contributions: J.S.

show abstract

“…Following biotic and abiotic stress, lipids can release FFAs (including free saturated fatty acids and free unsaturated fatty acids), and then the FFAs can be oxidized into a wide range of metabolites. The lipids can also be directly oxidized into oxylipin-containing lipids (Genva et al 2018). In this study, the total amount of oxylipincontaining glycolipids (MGDGs and DGDGs) and oxylipin-containing PGs increased at 1 h after treatment with oligochitosan; however, glycolipids (MGDGs), PGs, and FFAs (C14:0, C16:0, C16:2, C16:3, C18:0, C18:2 and C18:3) decreased at the same time.…”

Section: Discussionmentioning

confidence: 56%

“…The results suggested that the synthesized glycerolipids were oxidized to oxylipin-containing glycerolipids, rather than they released FFAs in response to oligochitosan treatment. Plants produced high amount of oxylipin-containing lipids under stress conditions (Genva et al 2018). Buseman et al (2006) found that the content of oxylipin-containing lipids (glycerolipids containing oxophytodienoic acid and dinor-oxophytodienoic acid) was higher in Arabidopsis wounded leaves than in control ones.…”

Section: Discussionmentioning

confidence: 99%

Changes of lipid metabolism of Arabidopsis thalianain response to oligochitosan treatment

Wang¹,

Su²,

Li³

et al. 2020

Biol. plant.

View full text Add to dashboard Cite

Lipids are vital cellular constituents in plant, and lipid peroxidation metabolites are critical defence substances in plants. In this study, mass spectrometry along with projections to latent structures discriminant analysis (PLS-DA) was used to detect lipid metabolism changes in Arabidopsis thaliana in response to oligochitosan (an effective resistance elicitor for the control of plant diseases). The PLS-DA showed that lipid metabolites of Arabidopsis thaliana were influenced by oligochitosan treatment. The total content of oxylipin containing monogalactosyldiacylglycerols, oxylipin-containing digalactosyldiacylglycerols, and oxylipin-containing phosphatidylglycerols increased firstly (after 1 h), and then decreased with the increase of oligochitosan treatment duration. In contrast, the total content of monogalactosyldiacylglycerols, phosphatidylcholines, phosphatidyl-ethanolamine, and phosphatidylglycerols decreased firstly, and then increased with the increase of oligochitosan treatment duration. The amounts of free fatty acids (C16:2, C16:3, C18:2, and C18:3) were lower after treatment with oligochitosan for 1, 3, and 6 h than in the control, while the production of volatile organic compounds such as 2-hexenal (except for 3 h) and nonanal was higher than in the control. In conclusion, lipid metabolites of Arabidopsis thaliana were influenced by oligochitosan treatment, and the synthesized lipids and oxylipin-containing lipids were remodelled and free fatty acids was metabolized to volatile organic compounds.

show abstract

New insights into the biosynthesis of esterified oxylipins and their involvement in plant defense and developmental mechanisms

Cited by 49 publications

References 100 publications

Phytohormonal cross-talk modulate Bipolaris sorokiniana (Scc.)interaction with Zea mays

Phytohormonal cross-talk modulate Bipolaris sorokiniana (Scc.)interaction with Zea mays

JASSY, a chloroplast outer membrane protein required for jasmonate biosynthesis

Changes of lipid metabolism of Arabidopsis thalianain response to oligochitosan treatment

Contact Info

Product

Resources

About