New insights into the molecular basis of mammalian sperm-egg membrane interactions

Vjugina, Ulyana

doi:10.2741/2693

Cited by 62 publications

(51 citation statements)

References 97 publications

(135 reference statements)

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“…The sperm and the plasma membrane of the egg bind and fuse. [141][142][143] Several genes have been implicated to play a role in post-testicular maturation, capacitation and fertilization using mouse model studies Mouse models in male fertility research D Jamsai and MK O'Bryan 147 (Table 3). In contrast to premeiotic and meiotic defects, the majority of genes critical for postmeiotic and post-testicular defects exhibit male-specific infertility defect.…”

Section: -114mentioning

confidence: 99%

Mouse models in male fertility research

Jamsai¹,

O'Bryan²

2010

Asian J Androl

115

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Limited knowledge of the genetic causes of male infertility has resulted in few treatment and targeted therapeutic options. Although the ideal approach to identify infertility causing mutations is to conduct studies in the human population, this approach has progressed slowly due to the limitations described herein. Given the complexity of male fertility, the entire process cannot be modeled in vitro. As such, animal models, in particular mouse models, provide a valuable alternative for gene identification and experimentation. Since the introduction of molecular biology and recent advances in animal model production, there has been a substantial acceleration in the identification and characterization of genes associated with many diseases, including infertility. Three major types of mouse models are commonly used in biomedical research, including knockout/knockin/gene-trapped, transgenic and chemical-induced point mutant mice. Using these mouse models, over 400 genes essential for male fertility have been revealed. It has, however, been estimated that thousands of genes are involved in the regulation of the complex process of male fertility, as many such genes remain to be characterized. The current review is by no means a comprehensive list of these mouse models, rather it contains examples of how mouse models have advanced our knowledge of post-natal germ cell development and male fertility regulation.

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Section: -114mentioning

confidence: 99%

Mouse models in male fertility research

Jamsai¹,

O'Bryan²

2010

Asian J Androl

115

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“…Although a considerable amount of knowledge has been gained especially in human and mouse (Almeida et al 1995, Bronson & Fusi 1996, Fusi et al 1996a, 1996b, Nixon et al 2007, Vjugina & Evans 2008, the exact mechanisms of the molecules involved in bovine fertilization are not unequivocally identified at present. Carbohydrates and glycoproteins are assumed to modulate adhesion and binding events during consecutive reproductive processes, like sperm-oviduct adhesion (Lefebvre et al 1997, Suarez et al 1998, Revah et al 2000, Talevi & Gualtieri 2001, Sostaric et al 2005, Ignotz et al 2007, sperm-oocyte interactions (Tulsiani et al 1997, Gougoulidis et al 1999, Amari et al 2001, Tanghe et al 2004a, 2004b, and embryo implantation (Whyte & Allen 1985, Biermann et al 1997.…”

Section: Introductionmentioning

confidence: 99%

Expression and putative function of fibronectin and its receptor (integrin α5β1) in male and female gametes during bovine fertilization in vitro

Thys¹,

Nauwynck²,

Maes³

et al. 2009

Reproduction

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Fibronectin (Fn) is a 440 kDa glycoprotein assumed to participate in sperm-egg interaction in human. Recently, it has been demonstrated that Fn -when present during bovine IVF -strongly inhibits sperm penetration. The present study was conducted firstly to evaluate the expression of Fn and its integrin receptor (a 5 b 1 ) on male and female bovine gametes using indirect immunofluorescence and secondly, to determine the function of Fn during bovine IVF. Endogenous Fn was detected underneath the zona pellucida (ZP) and integrin a 5 on the oolemma of cumulus-denuded oocytes. Bovine spermatozoa displayed integrin a 5 at their equatorial segment after acrosome reaction. We established that the main inhibitory effect of exogenously supplemented Fn was located at the sperm-oolemma binding, with a (concurrent) effect on fusion, and this can probably be attributed to the binding of Fn to spermatozoa at the equatorial segment, as shown by means of Alexa Fluor 488-conjugated Fn. Combining these results, the inhibitory effect of exogenously supplemented Fn seemed to be exerted on the male gamete by binding to the exposed integrin a 5 b 1 receptor after acrosome reaction. The presence of endogenous Fn underneath the ZP together with integrin a 5 expression on oolemma and acrosome-reacted (AR) sperm cell surface suggests a 'velcro' interaction between the endogenous Fn ligand and corresponding receptors on both (AR) sperm cell and oolemma, initiating sperm-egg binding.

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“…Cd9-null eggs can be fertilized and undergo normal embryonic development when sperm-egg membrane interactions are bypassed by intracytoplasmic sperm injection (ICSI) (60,61). Interestingly, CD9 interacts in vitro with pregnancy-specific glycoprotein PSG17, a member of the carcinoembryoinc antigen subfamily of IgSFs that has not been detected on sperm (50). IZUMO1 is also an IgSF member, leading to the hypothesis that CD9 and IZUMO1 interact, but currently there is no evidence to support this (16,50).…”

Section: Sperm-egg Fusion In Micementioning

confidence: 99%

“…Tetraspanins contain four transmembrane domains with one large extracellular loop and another smaller loop. They interact with other molecules in the plasma membrane including other tetraspanins, integrins, growth factor receptors, IgSF proteins, complement regulatory proteins, and proteoglycans, and they are thought to regulate plasma membrane functions by creating networks of proteins through their cis interactions (50). Cd9 -/-female mice are severely subfertile because of defective spermegg interactions (59,60).…”

Section: Sperm-egg Fusion In Micementioning

confidence: 99%

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Fertilization and the oocyte-to-embryo transition in C. elegans

Marcello¹,

Singson²

2010

BMB Reports

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Fertilization is a complex process comprised of numerous steps. During fertilization, two highly specialized and differentiated cells (sperm and egg) fuse and subsequently trigger the development of an embryo from a quiescent, arrested oocyte. Molecular interactions between the sperm and egg are necessary for regulating the developmental potential of an oocyte, and precise coordination and regulation of gene expression and protein function are critical for proper embryonic development. The nematode Caenorhabditis elegans has emerged as a valuable model system for identifying genes involved in fertilization and the oocyte-to-embryo transition as well as for understanding the molecular mechanisms that govern these processes. In this review, we will address current knowledge of the molecular underpinnings of gamete interactions during fertilization and the oocyte-to-embryo transition in C. elegans. We will also compare our knowledge of these processes in C. elegans to what is known about similar processes in mammalian, specifically mouse, model systems. [BMB reports 2010; 43(6): 389-399]

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New insights into the molecular basis of mammalian sperm-egg membrane interactions

Cited by 62 publications

References 97 publications

Mouse models in male fertility research

Mouse models in male fertility research

Expression and putative function of fibronectin and its receptor (integrin α5β1) in male and female gametes during bovine fertilization in vitro

Fertilization and the oocyte-to-embryo transition in C. elegans

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