New Insights into the Role and Therapeutic Potential of Heat Shock Protein 70 in Bovine Viral Diarrhea Virus Infection

Chen, Nannan; Bai, Tongtong; Wang, Shuang; Wang, Huan; Wu, Yue; Liu, Yu; Zhu, Zhanbo

doi:10.3390/microorganisms11061473

Cited by 3 publications

(2 citation statements)

References 104 publications

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“…HSP70 was recently found to have important functions in different viruses [32,[55][56][57]. At present, HSP70 has been validated as a host antiviral target in dengue virus (DENV), bovine viral diarrhoea virus (BVDV), flavivirus, grass carp reovirus (GCRV) and respiratory syncytial virus (RSV) infections [33,52,56,58]. Different bacteria and viruses must rely on their hosts to replicate, which would help us identify the Achilles heel of many viruses.…”

Section: Discussionmentioning

confidence: 99%

Drug screening identified that handelin inhibits feline calicivirus infection by inhibiting HSP70 expression in vitro

Yan,

Yang,

Jiao

et al. 2024

Journal of General Virology

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Feline calicivirus (FCV) is considered one of the major pathogens of cats worldwide and causes upper respiratory tract disease in all cats. In some cats, infection is by a highly virulent strain of FCV (vs.-FCV), which can cause severe and fatal systemic disease symptoms. At present, few antiviral drugs are approved for clinical treatment against FCV. Therefore, there is an imminent need for effective FCV antiviral agents. Here, we used observed a cytopathic effect (CPE) assay to screen 1746 traditional Chinese medicine monomer compounds and found one that can effectively inhibit FCV replication, namely, handelin, with an effective concentration (EC50) value of approximately 2.5 µM. Further study showed that handelin inhibits FCV replication via interference with heat shock protein 70 (HSP70), which is a crucial host factor and plays a positive role in regulating viral replication. Moreover, handelin and HSP70 inhibitors have broad-spectrum antiviral activity. These findings indicate that handelin is a potential candidate for the treatment of FCV infection and that HSP70 may be an important drug target.

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Section: Discussionmentioning

confidence: 99%

Drug screening identified that handelin inhibits feline calicivirus infection by inhibiting HSP70 expression in vitro

Yan,

Yang,

Jiao

et al. 2024

Journal of General Virology

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“…Our ndings con rmed that the speci c role for HSP70 in BoDV-1 infection. HSP70 has been described in several virus infections (49)(50)(51). In some cases, the HSP70 can interact with viral polymerases directly to enhance the replication of the virus, or they can promote the formation of viral replication complexes and maintain the stability of complex proteins (26).…”

Section: Discussionmentioning

confidence: 99%

Inducing the Localization of HSP70 to Lipid Rafts by Borna Disease Virus 1 Promotes Its Invasion and Host Cells Apoptosis

Lei,

Yang,

et al. 2023

Preprint

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The Borna disease virus 1 (BoDV-1) is an emerging zoonotic virus causing severe and mostly fatal encephalitis in humans. Increasing evidence of human infections by BoDV-1 has highlighted the importance of antiviral drugs against BoDV-1. However, the process of BoDV-1 infection and its pathogenic mechanism remain elusive and there are few drugs targeting BoDV-1 infection. Our previous research suggests that BoDV-1 infects cells through lipid-related pathways. Heat shock protein 70 (HSP70) in lipid rafts (LR) has been identified to participate in various viral infections. In this study, we reported that the LR-HSP70-Caspase-3 axis engages BoDV-1 invasion and host cell apoptosis. Mechanistically, the LR inhibitor, Methyl-β-cyclodextrin (MβCD), interferes the localization HSP70 to LR and prevents BoDV-1 entry without reducing cell viability.Furthermore, we confirmed that the HSP70 is recruited into LR after BoDV-1 invasion by HSP70 knockdown and overexpression assays. And the migration of HSP70 to LR weakened the interaction between HSP70 and Caspase-3, which reducing the chaperone protective ability of HSP70 to Caspase-3, increasing the intracellular active Caspase-3 and promoting host cell apoptosis. Importantly, the MβCD treatment can effectively alleviate the symptoms and pathological changes in a model of BoDV-1 encephalitis, suggesting a significant antiviral effect in vivo. Our report reveals the process of BoDV-1 invasion and the mechanisms by which MβCD inhibits the infection, offering potential guidance for the development in the targeted treatment of BoDV-1 infection.

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Therapeutic Phages as Modulators of the Immune Response: Practical Implications

Górski,

Międzybrodzki,

Jończyk-Matysiak

et al. 2023

Clinical Infectious Diseases

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While the medical community awaits formal proof of the efficacy of phage therapy, as is required by evidence-based medicine, existing data suggest that phages could also be applied based on their non-antibacterial action, especially phage-mediated immunomodulation. Promising avenues have been revealed by findings indicating that phages may mediate diverse actions in the immune system, while the list of phages able to dampen the aberrant immunity associated with a variety of disorders continuously grows. Here we summarize what is known in this field and possible options for the future. While available data are still scarce and preliminary, it appears that “phage repurposing” is worthy of more research, which could reveal new perspectives on applying phage therapy in contemporary medicine.

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New Insights into the Role and Therapeutic Potential of Heat Shock Protein 70 in Bovine Viral Diarrhea Virus Infection

Cited by 3 publications

References 104 publications

Drug screening identified that handelin inhibits feline calicivirus infection by inhibiting HSP70 expression in vitro

Drug screening identified that handelin inhibits feline calicivirus infection by inhibiting HSP70 expression in vitro

Inducing the Localization of HSP70 to Lipid Rafts by Borna Disease Virus 1 Promotes Its Invasion and Host Cells Apoptosis

Therapeutic Phages as Modulators of the Immune Response: Practical Implications

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