New insights into virulence mechanisms of rice pathogen Acidovorax avenae subsp. avenae strain RS-1 following exposure to ß-lactam antibiotics

Li, Bin; Ge, Mengyu; Zhang, Yang; Wang, Li; Ibrahim, Muhammad; Wang, Yanli; Sun, Guochang; Chen, Gongyou

doi:10.1038/srep22241

Cited by 7 publications

(14 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We propose that antibiotics induce the expression and secretion of T6SS and might make the attacker T6SS + strains more aggressive in the competition for growth. A previous report (Li et al, 2016 ) has shown a similar phenomenon in Acidovorax avenae subsp. avenae (Aaa) strain RS-1.…”

Section: Discussionsupporting

confidence: 62%

“…ClpV (a class of ATPases) is required to disassemble the contracted VipA/VipB sheath (Bönemann et al, 2009 ; Basler and Mekalanos, 2012 ). Two core proteins, Hcp and VgrG, have frequently been used as markers to investigate the T6SS translocation of secreted effectors (Pukatzki et al, 2006 , 2009 ; Li et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

“…However, little is known about the antibacterial function of T6SS in K. pneumoniae (Hood et al, 2017 ). A recent finding suggested that the rice pathogenic bacterium Acidovorax avenae strain RS-1 exposure to the β-lactam antibiotics enhanced the virulence of T6SS (Li et al, 2016 ). Thus, a better understanding of the role of T6SS in CRKP under antibiotic stress is warranted.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Identification and Characterization of an Antibacterial Type VI Secretion System in the Carbapenem-Resistant Strain Klebsiella pneumoniae HS11286

Liu

et al. 2017

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

The type VI secretion system (T6SS) is a class of sophisticated cell contact-dependent apparatus with anti-eukaryotic or anti-bacterial function. Klebsiella pneumoniae is one of the most common bacterial pathogens with resistance to the carbapenem antibiotics. However, little is known about the antibacterial T6SS in K. pneumoniae. Using core-component protein searches, we identified a putative T6SS gene cluster on the chromosome of the carbapenemase-producing K. pneumoniae (CRKP) strain HS11286. Intraspecies and interspecies competition assays revealed an antibacterial function of the HS11286 T6SS. The phospholipase Tle1KP was found to be an effector protein that is transferred by T6SS. The overexpression of this effector gene in the periplasm caused severe growth inhibition of Escherichia coli. A sub-inhibitory concentration of β-lactam antibiotics stimulated the expression and secretion of the HS11286 T6SS and enhanced T6SS-dependent killing. It suggested that the antibiotics might be an impact factor for the T6SS secretion and antibacterial activity.

show abstract

Section: Discussionsupporting

confidence: 62%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Identification and Characterization of an Antibacterial Type VI Secretion System in the Carbapenem-Resistant Strain Klebsiella pneumoniae HS11286

Liu

et al. 2017

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Thus, the role of ClpB in bacterial virulence may be at least partially due to the two putative effector proteins. This was justified by previous data, which clearly demonstrated that Hcp and VgrG could influence bacterial growth, motility, biofilm formation and T6SS gene expression in RS‐1 (Li et al ., , ).…”

Section: Discussionmentioning

confidence: 99%

“…According to previous studies (Li et al, 2014(Li et al, , 2016, the pathogenicity of Aaa RS-1 in rice is highly associated with a functional Type VI secretion system (T6SS), which has been predicted in more than 25% of fully sequenced bacterial genomes (Cascales, 2008;Pukatzki et al, 2009;Miyata et al, 2011;Liu et al, 2012a). In addition, T6SS genes have also been found to be involved in the adaptation of Aaa RS-1 to different environmental stresses such as high salt, low temperature, b-lactam antibiotics, as well as H 2 O 2 and paraquat-induced oxidative stress (Cui et al, 2015;Li et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Characterization and functional analysis of clpB gene from Acidovorax avenae subsp. avenae RS‐1

Zhang

et al. 2017

Plant Pathology

View full text Add to dashboard Cite

The type VI secretion system (T6SS) has been reported to be highly associated with various cellular activities in strain RS‐1 of Acidovorax avenae subsp. avenae (Aaa), the pathogen of bacterial brown stripe of rice. However, the role of the clpB gene that presents in the T6SS gene cluster in Aaa pathogenicity has not been clarified. The aim of the current study was to characterize the function of clpB and to investigate its contribution to bacterial pathogenesis using insertional deletion mutation and complementation approaches. The results indicated that mutation of clpB significantly affected bacterial growth, virulence, exopolysaccharide (EPS) production, biofilm formation and expression of 13 other T6SS genes of Aaa RS‐1. The reduction of virulence may be also partially due to the change in EPS composition, which was characterized by the Fourier transform infrared (FTIR) spectra. Furthermore, analysis of protein homology modelling showed that the structure of ClpB is different from those of the other T6SS components. In addition, structural difference was observed between ClpB and Type IV pili (TFP) as well as Type IV pilus biogenesis proteins (PilP), whose functions are similar to ClpB. Taken together, this study demonstrated that the clpB gene plays a key role in Aaa bacterial virulence.

show abstract

IcmF and DotU are required for the virulence of Acidovorax oryzae strain RS-1

Wang

Chen

et al. 2018

Arch Microbiol

View full text Add to dashboard Cite

Acidovorax oryzae (Ao) cause bacterial brown stripe in rice that result in great economic loss. However, the pathogenic mechanism of this rice bacterial pathogen still remains unclear. Interestingly, transcriptomic and proteomic analysis of in vivo infection indicate that the pathogenicity of Ao strain RS-1 may be associated with the type six secretion system (T6SS), which was identified by in silico comparative genomic analysis in our previous studies. This makes it necessary to further examine the role of each core component of T6SS in the pathogenicity of Ao strain RS-1 to rice plants. Results from this study highlight the mutual interaction between IcmF and DotU, which was determined by bacterial two-hybrid and bimolecular fluorescence complementation assays. Furthermore, a difference was observed in bacterial pathogenicity, biofilm formation, secreted proteins identified by LC-MS/MS analysis and the expression of T6SS other genes examined by quantitative real-time PCR between the wild-type and both single-gene knockout mutants, which were respectively constructed based on the insertional mutagenesis of Ao strain RS-1 in this study. Overall, our results clearly revealed the importance of T6SS icmF and dotU in pathogenicity of Ao strain RS-1 to rice plants.

show abstract

New insights into virulence mechanisms of rice pathogen Acidovorax avenae subsp. avenae strain RS-1 following exposure to ß-lactam antibiotics

Cited by 7 publications

References 47 publications

Identification and Characterization of an Antibacterial Type VI Secretion System in the Carbapenem-Resistant Strain Klebsiella pneumoniae HS11286

Identification and Characterization of an Antibacterial Type VI Secretion System in the Carbapenem-Resistant Strain Klebsiella pneumoniae HS11286

Characterization and functional analysis of clpB gene from Acidovorax avenae subsp. avenae RS‐1

IcmF and DotU are required for the virulence of Acidovorax oryzae strain RS-1

Contact Info

Product

Resources

About