New Insights into Vitamin K—From Its Natural Sources through Biological Properties and Chemical Methods of Quantitative Determination

Wianowska, Dorota; Bryshten, Iryna

doi:10.1080/10408347.2022.2121599

Cited by 8 publications

(8 citation statements)

References 99 publications

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“…Vitamin K is crucial in blood coagulation, bones calcification, brain and kidney function and is involved in maintaining human health through its large spectrum of anticancer, antibacterial and antiviral activities [1][2][3][4]. It is also involved in cellular respiration, photosynthetic mechanisms, and oxidative phosphorylation [5].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical biosensor based on NAD(P)H-dependent Quinone Reductase for rapid and efficient detection of vitamin K3

Khalife,

Stankovic,

Stankovic

et al. 2023

Preprint

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Vitamin K refers to a group of vitamins that play an important role in blood coagulation and regulation of bone and vascular metabolism. However, vitamin K3 may give severe side effects in animal and humans when improperly added to food and feed due to its toxicity. Here, an electrochemical biosensor, based on the YaiB NADPH-dependent quinone reductase from Lactococcus lactis (YaiB), was developed to achieve rapid and redox probe-free detection of vitamin K3. First, we demonstrated the ability of the carbon electrode to distinguish between 1,4-benzoquinone and hydroquinone. Then, we engineered YaiB to work as a bioreceptor immobilized at the electrode and we demonstrated its sensitivity and specificity to reduce vitamin K3. Finally, to demonstrate the practical potential of the biosensor, we tested it directly in spiked milk samples, achieving 15-minute quantification of the vitamin K3. The limit of detection was 0.18microM and 0.86 microM in buffer and milk, respectively.

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Section: Introductionmentioning

confidence: 99%

Electrochemical biosensor based on NAD(P)H-dependent Quinone Reductase for rapid and efficient detection of vitamin K3

Khalife,

Stankovic,

Stankovic

et al. 2023

Preprint

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“…According to [ 2 , 3 , 4 , 5 , 6 , 8 ] these properties are more specific to MK-7, hence the growing interest in determining this compound is observed. This interest goes beyond the strictly scientific framework, and currently, the analysis of vitamin K2MK-7 becomes an important diagnostic and preventive tool [ 9 ]. This is because the half-life of MK-7 is several days, not 1–2 h as in the case of K1, and the longer half-life means that it stays longer in the blood and is much more available to extrahepatic tissues [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

“…Among them, liquid chromatography (LC) with the fluorescent detection after the post-column zinc reduction to a stable hydroquinone derivative, is the most commonly applied [ 12 , 16 , 17 ]. The most recent one is LC coupled with mass spectrometry (MS) or tandem mass spectrometry (MS/MS) detection [ 9 , 15 , 18 , 19 , 20 ]. These techniques have great analytical capabilities; however, are insufficient to determine a compound at low concentration levels in a complex natural matrix [ 21 , 22 , 23 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

“…Most approaches for the determination of K2MK-7 use solid-phase extraction (SPE) and/or liquid–liquid extraction (LLE), with the most commonly used extractants being those with higher partition coefficients ( log P ), such as diethyl ether (0.9), dichloromethane (1.5), ethyl acetate (0.7), toluene (2.7), chloroform (2.3), n -hexane (3.9), and cyclohexane (3.4) [ 12 , 17 , 18 , 19 , 20 , 25 , 26 ]. These approaches use relatively large volumes of solvents (4–8 mL) in a single extraction step, with several steps usually being used to increase extraction efficiency [ 9 ]. These are also time-consuming procedures, usually taking several dozen minutes, which significantly reduces the throughput of the laboratory.…”

Section: Introductionmentioning

confidence: 99%

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Development, Validation, and Two-Year Application of Rapid and Simple LC-MS/MS-Based Method for the Determination of K2MK-7 in Blood Samples

Paprotny,

Szewczak,

Bryshten

et al. 2023

Molecules

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Biological properties of menaquinone-7, one of the vitamin K2 vitamers (K2MK-7), both those proven and those that remain to be investigated, arouse extensive interest that goes beyond the strictly scientific framework. The most important of them is the prevention of age-related diseases, considering that we live in the times identified as the era of aging societies and many people are exposed to the vitamin K2MK-7 deficiency. Therefore, an effective analytical protocol that can be adopted as a diagnostic and preventive analytics tool is needed. Herein, a simple sample preparation method followed by the liquid chromatography-tandem mass spectrometry-based method (LC-MS/MS), was used for the selective and sensitive determination of K2MK-7 in serum samples. Under the optimized conditions, using 500 µL of serum and the same amount of n-hexane, the reproducibility and the accuracy were obtained in the ranges of 89–97% and 86–110%, respectively, and the limit of detection value was 0.01 ng/mL. This method was used for the routine analysis. Statistical interpretation of the data from 518 samples obtained during 2 years of practice allowed for obtaining information on the content and distribution of K2MK-7 in the Polish population, broken down by the sex and age groups.

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“…The growing proportion of elderly people in many world populations and the emergence of various age-related metabolic disorders increase consumer health awareness and interest in a healthy diet rich in compounds with significant health benefits [ 1 ]. One such compound is quercetin, a polyphenolic antioxidant commonly consumed in many fruits and vegetables.…”

Section: Introductionmentioning

confidence: 99%

Correlation Study of Biological Activity with Quercetin and Phenolics Content in Onion Extracts

2022

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In this study it was shown that the fungistatic and antioxidant activities of onion extracts are related to the type of liquid used as the extractant and the technique of its preparation. A change in the antioxidant properties of white and red onion extracts was demonstrated with the change of the temperature of the pressurized hot water extraction process, which can be easily related to the changes accompanying the process of thermal processing of vegetables and fruits during cooking. Owing to the experimental and mathematical approaches concerning both the main and characteristic components of onions, i.e., quercetin and phenols, respectively, with the biological activity of the extracts, it was possible to demonstrate the significant share of these compounds in the antifungal and antioxidant properties of the extracts. Considering that the research was carried out, inter alia, on onion husks, demonstrating a very high potential of biological properties of this waste material from agricultural production, the research results presented in the paper should encourage the popularization of the use of this so far underestimated raw material for the production of various functional materials.

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New Insights into Vitamin K—From Its Natural Sources through Biological Properties and Chemical Methods of Quantitative Determination

Cited by 8 publications

References 99 publications

Electrochemical biosensor based on NAD(P)H-dependent Quinone Reductase for rapid and efficient detection of vitamin K3

Electrochemical biosensor based on NAD(P)H-dependent Quinone Reductase for rapid and efficient detection of vitamin K3

Development, Validation, and Two-Year Application of Rapid and Simple LC-MS/MS-Based Method for the Determination of K2MK-7 in Blood Samples

Correlation Study of Biological Activity with Quercetin and Phenolics Content in Onion Extracts

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