New Insights of Human Parvovirus B19 in Modulating Erythroid Progenitor Cell Differentiation

Feng, Shuwen; Zeng, Dongxin; Zheng, Jiandong; Zhao, Dongchi

doi:10.1089/vim.2020.0013

Cited by 3 publications

(3 citation statements)

References 96 publications

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“…The data are derived from the results of three independent experiments, LSD t-test after ANOVA analyses, *p < 0.05. [27]. The evolution of erythroid cells contains the different stages including hematopoietic stem cells (HSC), common myeloid progenitors (CMP), megakaryo-erythroid progenitor (MEP), BFU-E, CFU-E, proerythroblast, erythroblast, reticulocyte, and eventually red blood cell.…”

Section: Discussionmentioning

confidence: 99%

Human Parvovirus B19 Nonstructural Protein 1 Regulates GATA1 Expression via the Notch Signaling Pathway in K562 Cell Line

Zeng¹,

Zheng²,

Feng³

et al. 2022

Front. Biosci. (Landmark Ed)

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Background: Human parvovirus B19 (B19) infection can affect the hematopoietic arrest in fetus by hindering the differentiation and maturation of erythroid progenitor cells. B19 nonstructural protein 1 (NS1) has been shown to inhibit the differentiation of erythroid progenitor cells. The goal of this study is to explore the role of B19 NS1 in the regulation of GATA1 and Notch signaling pathway in hematopoietic cells. Methods: The B19 NS1 expression plasmid was reconstituted, and the possibility of NS1 regulating GATA1 and GATA2 expression modulated by Notch-Hes pathway was tested by qRT-PCR and western blot. Immunofluorescence assays were conducted to visualize pNS1 in K562 cells. Results: We demonstrate that B19 NS1 inhibited GATA1 and induced Hes1/Hes5, which is involved in the activation of Notch signaling pathway. Meanwhile, NS1 exhibited promoting effects on GATA2 expression. Activation of the Notch signaling pathway up-regulated its downstream transcriptional repressor family Hes, thereby inhibiting the expression of GATA gene in K562 cells. Conclusions: The results show that B19 NS1 protein negatively regulates GATA1 related nuclear transcription and may interfere with hematopoietic cell differentiation.

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Section: Discussionmentioning

confidence: 99%

Human Parvovirus B19 Nonstructural Protein 1 Regulates GATA1 Expression via the Notch Signaling Pathway in K562 Cell Line

Zeng¹,

Zheng²,

Feng³

et al. 2022

Front. Biosci. (Landmark Ed)

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“…Parvovirus B19 primarily targets immature cells, inducing TAC through tumor necrosis factor and stimulating autoimmune reactions to affect cell lines 3,7. The prevalence of parvovirus B19 antibodies found in serum sampling increases exponentially with age, from 2% in children under age 5 years to 85% of older adults 3,5,8.…”

Section: Discussionmentioning

confidence: 99%

“…2,3,5 Parvovirus B19 primarily targets immature cells, inducing TAC through tumor necrosis factor and stimulating autoimmune reactions to affect cell lines. 3,7 The prevalence of parvovirus B19 antibodies found in serum sampling increases exponentially with age, from 2% in children under age 5 years to 85% of older adults. 3,5,8 Although erythema infectiosum facial rash is classically associated with parvovirus B19, it is rare in parvovirus B19-induced TAC, rendering it a challenging diagnosis without distinguishing features.…”

Section: Discussionmentioning

confidence: 99%

What is causing this infant's febrile lethargy?

Carius

Bridwell²,

Rozman³

2022

Jaapa

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GATA1 transcription factor targets the gene expression of B19 virus in HEK293 cell line

Atashi,

Jafaripour,

Froughi

et al. 2024

Annals of Medicine &Amp; Surgery

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Backgrond/Aim: B19 virus (B19V) is a single strand DNA virus that has specific tropism to erythroid progenitor cells (EPCs). The virus enters to the cells via P antigen and coreseptors and induces infection and cell apoptosis. GATA1 has a high expression in EPC and is a critical transcription factor for the cells development and differentiation. As human EPCs are the main target of the virus infection that have high expression of GATA-1 as the critical transcription factor, the aim of this study was to investigate the effect of GATA1 cotransfection with B19V genome on expression of the viral mRNAs in HEK293 as non-permissive cell line to the virus that had no mRNA expression of GATA-1. Methods: HEK293 cells were transfected with pHI0 plasmid containing B19V genome and the plasmid of GATA1 genome. The quantity of B19V mRNAs (NS1, 7.5 kDa, 11 kDa) expression was evaluated after 24h of transfection. Results: The results showed a statistically significant increase in fold change expression of (NS1 ∽ 12.3, VP1 ∽ 27.6, 11kb protein ∽ 38) in cotransfected cells with GATA1 and B19 plasmids compare to control group (P<0.05). Conclusion: This research showed transfected cells with GATA1had elevation in expression of the B19V genes mRNAs in a non-permissive cell. This result may show a role of GATA1 as a critical transcription factor in support of the virus infection in EPCs. This suggests that GATA1 may potentially sport B19V replication or gene expression.

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New Insights of Human Parvovirus B19 in Modulating Erythroid Progenitor Cell Differentiation

Cited by 3 publications

References 96 publications

Human Parvovirus B19 Nonstructural Protein 1 Regulates GATA1 Expression via the Notch Signaling Pathway in K562 Cell Line

Human Parvovirus B19 Nonstructural Protein 1 Regulates GATA1 Expression via the Notch Signaling Pathway in K562 Cell Line

What is causing this infant's febrile lethargy?

GATA1 transcription factor targets the gene expression of B19 virus in HEK293 cell line

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