New Insights on Optical Biosensors: Techniques, Construction and Application

Martins, Tatiana Duque; Ribeiro, Antonio Carlos Chaves; Camargo, Henrique Santiago de; Filho, Paulo Alves da Costa; Cavalcante, Hannah Paula Mesquita; Dias, Diogo Lopes

doi:10.5772/52330

Cited by 18 publications

(6 citation statements)

References 73 publications

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“…While the total concentration of IgG found in the adult plasma is about 7 mg/mL, IgG antibodies generated from different infections have significantly lower concentrations at the onset and exhibit unique chemical signatures . This underscores the need for an infection specific detection of IgG at low concentrations with high sensitivity. − Conventionally, antibody detection is done using the techniques like polymerase chain reaction (PCR), enzyme-linked immunosorbent assay (ELISA), or Western blot that are reliable, however, they are time-consuming processes, prone to contamination, and require high volumes of samples for definitive evaluation. − Alternatively, optical techniques for detection of biomolecules offer the benefits of faster sensing and can be done using a fraction of the sample quantity. − With label-free detection, the concerns of affecting antibody binding affinities and introducing undesired interactions or modifications is eliminated. This removes the restrictions that tag labels such as dyes give, where it can only see the binding at the very end or can potentially modify the sample outside of its’ natural state.…”

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confidence: 99%

Magnetoplasmons for Ultrasensitive Label-Free Biosensing

et al. 2021

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Early detection of immunoglobin G (IgG), a glycoprotein antibody produced in the serum due to various infections, is of paramount importance that will enable effective treatment, immunity assessment, and assist in monitoring outbreaks of contagious diseases. This work demonstrates the transverse magneto-optic Kerr effect (T-MOKE) based magnetoplasmons excited on a composite ferromagnetic/plasmonic grating as a highly sensitive, single wavelength, and target specific biosensing platform. The sharp T-MOKE sensitivity curve corresponding to reduced fwhm results in a two orders of magnitude enhancement in the resolving power compared to conventional propagating surface plasmon polariton (SPP), which is pivotal in identifying minute fluctuations in specific biomolecular concentrations. An order of magnitude improvement in antibody immunoglobin G (IgG) detection limit is observed compared to the SPP based sensing. A detection limit down to 10 ng/mL (66 pM) is achieved using the proposed T-MOKE technique. The results obtained provide compelling evidence of the significantly superior sensitivity and resolving power of the T-MOKE technique for the detection of Human IgG, and it is envisioned that this spectroscopy free, single wavelength measurement approach can be extended to detect biologically/chemically relevant molecules at lower concentrations for early biomedical diagnosis and therapy.

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confidence: 99%

Magnetoplasmons for Ultrasensitive Label-Free Biosensing

et al. 2021

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“…Initially, the model-based event detection method involves a signal-to-noise principles using laboratory and sensor test-loop evaluation. Indication of contamination events is derived from the chemical changes in background water quality signals [88,89], [137][138][139][140][141][142][143], [163], [167], [217], [244,245], [429,430] Sensor Placements Approach [372][373][374][375][376][377], [380][381][382][383][384][385][386][387][388], [391], [400][401][402][403][404][405][406][407], [434][435][436][437][438] Event Detection Model-based -High true positive alarm rate -Low false alarm detections -Fast response time -Complicated calibration process -Highly dependable on predictions and estimations -Computationally intensive…”

Section: Algorithmic Model-based Event Detectionmentioning

confidence: 99%

Detection of contaminants in water supply: A review on state-of-the-art monitoring technologies and their applications

Zulkifli

Rahim

Lau

2018

Sensors and Actuators B: Chemical

232

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a b s t r a c tWater monitoring technologies are widely used for contaminants detection in wide variety of water ecology applications such as water treatment plant and water distribution system. A tremendous amount of research has been conducted over the past decades to develop robust and efficient techniques of contaminants detection with minimum operating cost and energy. Recent developments in spectroscopic techniques and biosensor approach have improved the detection sensitivities, quantitatively and qualitatively. The availability of in-situ measurements and multiple detection analyses has expanded the water monitoring applications in various advanced techniques including successful establishment in hand-held sensing devices which improves portability in real-time basis for the detection of contaminant, such as microorganisms, pesticides, heavy metal ions, inorganic and organic components. This paper intends to review the developments in water quality monitoring technologies for the detection of biological and chemical contaminants in accordance with instrumental limitations. Particularly, this review focuses on the most recently developed techniques for water contaminant detection applications. Several recommendations and prospective views on the developments in water quality assessments will also be included.

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“…They allow highly specific and sensitive sensing of bacteria in a rapid, real-time and cost-effective fashion. In general, they can be divided into label-based (e.g., fluorescent) and label-free methods [ 109 , 110 ]. Plasmonic biosensors, relying on the use of surface plasmon resonance (SPR) or surface enhanced Raman spectroscopy (SERS), are nowadays frequently used for pathogen detection ( Figure 3 ) [ 42 ].…”

Section: Biosensors For Detecting Bacterial Cellsmentioning

confidence: 99%

Antimicrobial Peptides: Powerful Biorecognition Elements to Detect Bacteria in Biosensing Technologies

2018

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Bacterial infections represent a serious threat in modern medicine. In particular, biofilm treatment in clinical settings is challenging, as biofilms are very resistant to conventional antibiotic therapy and may spread infecting other tissues. To address this problem, biosensing technologies are emerging as a powerful solution to detect and identify bacterial pathogens at the very early stages of the infection, thus allowing rapid and effective treatments before biofilms are formed. Biosensors typically consist of two main parts, a biorecognition moiety that interacts with the target (i.e., bacteria) and a platform that transduces such interaction into a measurable signal. This review will focus on the development of impedimetric biosensors using antimicrobial peptides (AMPs) as biorecognition elements. AMPs belong to the innate immune system of living organisms and are very effective in interacting with bacterial membranes. They offer unique advantages compared to other classical bioreceptor molecules such as enzymes or antibodies. Moreover, impedance-based sensors allow the development of label-free, rapid, sensitive, specific and cost-effective sensing platforms. In summary, AMPs and impedimetric transducers combine excellent properties to produce robust biosensors for the early detection of bacterial infections.

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New Insights on Optical Biosensors: Techniques, Construction and Application

Cited by 18 publications

References 73 publications

Magnetoplasmons for Ultrasensitive Label-Free Biosensing

Magnetoplasmons for Ultrasensitive Label-Free Biosensing

Detection of contaminants in water supply: A review on state-of-the-art monitoring technologies and their applications

Antimicrobial Peptides: Powerful Biorecognition Elements to Detect Bacteria in Biosensing Technologies

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