New Insights on Plant Cell Elongation: A Role for Acetylcholine

Sansebastiano, Gian Pietro Di; Fornaciari, Silvia; Barozzi, Fabrizio; Piro, Gabriella; Arru, Laura

doi:10.3390/ijms15034565

Cited by 34 publications

(24 citation statements)

References 111 publications

(142 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Acetylcholine is a small neurotransmitter molecule, reportedly involved in regulation of growth and development by modulating a multitude of physio‐biochemical processes in plants and animals (Tretyn & Kendrick ; Sansebastiano et al . ). However, little evidence is available in the literature on the function of ACh in plant adaptation to salinity stress.…”

Section: Discussionmentioning

confidence: 97%

Beneficial role of acetylcholine in chlorophyll metabolism and photosynthetic gas exchange in Nicotiana benthamiana seedlings under salinity stress

et al. 2020

View full text Add to dashboard Cite

Acetylcholine (ACh) is believed to improve plant growth. However, regulation at biochemical and molecular levels is largely unknown.• The present study investigated the impact of exogenously applied ACh (10 µM) on growth and chlorophyll metabolism in hydroponically grown Nicotiana benthamiana under salt stress (150 mM NaCl).• Salinity reduced root hydraulic conductivity while ACh-treated seedlings exhibited a significant increase, resulting in increased relative water content. Salinity induced a reduction in chlorophyll biosynthetic intermediates, such as protoporphyrin-IX, Mgphotoporphyrin-IX and protochlorophyllide, which were significantly ameliorated in the presence of ACh. This influence of ACh on chlorophyll synthesis was confirmed by up-regulation of HEMA1, CHLH, CAO and POR genes. Gas exchange parameters, i.e. stomatal conductance, internal CO 2 concentration and transpiration rate, increased with ACh, thereby alleviating the salinity effects on photosynthesis. In addition, the salinity-induced enhancement of lipid peroxidation declined after ACh treatment through modulation of the activity of the assayed antioxidant enzymes (superoxide dismutase and peroxidase). Importantly, ACh significantly reduced the uptake of Na and increased uptake of K, resulting in a decline in the Na/K ratio.• Results of the present study indicate that ACh can be effective in ameliorating NaClinduced osmotic stress, altering chlorophyll metabolism and thus photosynthesis by maintaining ion homeostasis, hydraulic conductivity and water balance.

show abstract

Section: Discussionmentioning

confidence: 97%

Beneficial role of acetylcholine in chlorophyll metabolism and photosynthetic gas exchange in Nicotiana benthamiana seedlings under salinity stress

et al. 2020

View full text Add to dashboard Cite

show abstract

“…The drug BFA is often used to demonstrate the involvement of Golgi in vacuolar trafficking (for a review see [36]) but it is also acting on endosomal compartments and on recycling to the plasmalemma, causing the formation of BFA bodies [37]. Recent results in Nicotiana [17], indirectly confirmed in tomato [38], indeed suggest that GFPChi transits via an intermediate compartment independent from the trans -Golgi Network (TGN) but involved in the late events of BFA bodies formation.…”

Section: Chitinase Sorting a Hidden Pathmentioning

confidence: 99%

Contribution of Chitinase A’s C-Terminal Vacuolar Sorting Determinant to the Study of Soluble Protein Compartmentation

Stigliano

Sansebastiano

Neuhaus

2014

IJMS

Self Cite

View full text Add to dashboard Cite

Plant chitinases have been studied for their importance in the defense of crop plants from pathogen attacks and for their peculiar vacuolar sorting determinants. A peculiarity of the sequence of many family 19 chitinases is the presence of a C-terminal extension that seems to be important for their correct recognition by the vacuole sorting machinery. The 7 amino acids long C-terminal vacuolar sorting determinant (CtVSD) of tobacco chitinase A is necessary and sufficient for the transport to the vacuole. This VSD shares no homology with other CtVSDs such as the phaseolin’s tetrapeptide AFVY (AlaPheValTyr) and it is also sorted by different mechanisms. While a receptor for this signal has not yet been convincingly identified, the research using the chitinase CtVSD has been very informative, leading to the observation of phenomena otherwise difficult to observe such as the presence of separate vacuoles in differentiating cells and the existence of a Golgi-independent route to the vacuole. Thanks to these new insights in the endoplasmic reticulum (ER)-to-vacuole transport, GFPChi (Green Fluorescent Protein carrying the chitinase A CtVSD) and other markers based on chitinase signals will continue to help the investigation of vacuolar biogenesis in plants.

show abstract

“…Because rooting (in vivo or in vitro) is mainly affected by auxin, the effectiveness of Ach in promoting root grown Table 1 Percentage of germinated (%G), germination speed index (GSI), first count (%FC), strong normal seedlings (SNS), weak normal soybean seedlings (WNS), length shoot (LS), length root (LR), length total (LT), dry matter of shoot (DMS), root dry mass (RDM), total dry matter (DMT) and relationship between root and shoot (R/S) soy genotype Intacta, subjected to different concentrations of acetylcholine and different osmotic conditions suggests an interesting possibility on the mechanism of action of Ach and its involvement with auxin (Baluska et al 2005). Some experiments have shown that Ach combined with auxin increases the expression of the expansin LeEXPA2 gene in the hypocotyls of tomatoes, when compared to auxin and Ach treatments separately (Di-Sansebastiano et al 2014). Expansins are known to be involved in cell elongation, being codified by a multigene family, where LeEXPA2 is included, which are regulated by auxin in the growing regions of young tomato hypocotyl (Caderas et al 2000).…”

Section: Discussionmentioning

confidence: 99%

Mitigating effects of acetylcholine supply on soybean seed germination under osmotic stress

2017

View full text Add to dashboard Cite

Acetylcholine (Ach) is a common neurotransmitter in animals, also synthesized in plants, which can have an influence on plant response to stress, also acting as a signaling molecule between root and shoot. The objective of this study was to analyze the possible mitigating effects of exogenous application of Ach on soybean germination under different levels of osmotic potential. The experiments were conducted with soybean [Glycine max (L.) Merrill] genotype Intacta. The seeds were first treated with Ach solutions with the following concentrations: 0.0 (control); 0.5; 1. 0 and 2.0 mM. Then, the seeds were subjected to two water potentials,-0.5 and-1.0 MPa, reached by using mannitol solutions, for the induction of osmotic stress, and a control condition with distilled water. Thus, 12 treatments were established in a double factorial 4 9 3, with 4 levels of Ach and 3 osmotic potential treatments (0.0,-0.5 and-1.0 MPa) with four replicates per treatment. The results showed that the concentration of 1.0 mM Ach, without osmotic stress, presented higher values for total dry mass of the seedlings compared to the control treatment (without Ach supply). In the treatments conducted to test the effectiveness of Ach on the mitigation of severe osmotic stress effects (-1.0 MPa), results showed that the concentration of 0.5 mM Ach showed positive results for the following parameters; dry weight of shoot, root dry weight, total dry mass, which were significantly higher than treatment under 1.0 MPa.

show abstract

New Insights on Plant Cell Elongation: A Role for Acetylcholine

Cited by 34 publications

References 111 publications

Beneficial role of acetylcholine in chlorophyll metabolism and photosynthetic gas exchange in Nicotiana benthamiana seedlings under salinity stress

Beneficial role of acetylcholine in chlorophyll metabolism and photosynthetic gas exchange in Nicotiana benthamiana seedlings under salinity stress

Contribution of Chitinase A’s C-Terminal Vacuolar Sorting Determinant to the Study of Soluble Protein Compartmentation

Mitigating effects of acetylcholine supply on soybean seed germination under osmotic stress

Contact Info

Product

Resources

About