New investigations into the stability of Mesna using LC-MS/MS and NMR

Abstract

3 It is important for sarcoma patients to receive the correct dose of Mesna as an adjuvant with Ifosfamide to reduce the risk of haemorrhagic cystitis. This paper describes a study conducted to evaluate the physicochemical stability of Mesna for injection formulation over 14 days.Mesna samples (n=4, 20mg/mL) were incubated in glass vials at 37+0.5ºC. Mesna concentrations were determined by LC-MS/MS, and NMR was used to detect degradation products. Evaporative losses and pH were also monitored.Our results differed from those published in existing literature. Both LC-MS/MS and NMR indicated that Mesna was unstable. The mean percentage decrease in Mesna concentration was 40% by day 14 of the analysis. The presence of Mesna's dimer Dimesna was detected on day 0 and its concentration increased over time. Dimesna was the only byproduct identified To conclude, both LC-MS/MS and NMR analyses confirmed the instability of Mesna and its conversion into Dimesna.

Cytoprotective Antioxidant, Anti-Inflammatory, and Antifibrotic Impact of Celery Seed Oil and Manuka Honey Against Cyclophosphamide-Induced Cystitis in Rabbits

“…On the other hand, mesna is transformed into dimesna, which is rapidly excreted in urine to create a nontoxic, inert dimer (thioether) on the mucosal surface of UB by direct coupling of dimesna with acrolein [48]. Lack of mesna antioxidant and anti-inflammatory activity limits its effectiveness, leading to ineffective prevention of HC in 25% of cases treated with CP.…”

A novel gold-nanocluster-based fluorescent sensor for detection of sodium 2-mercaptoethanesulfonate

“…Preparation of (a) His-Au NCs and (b) MES-Au NCs (a) Au nanoclusters were synthesized by blending histidine and chloroauric acid as reported previously. 25,26 Briey, chloroauric acid (5 mL, 0.01 M) was added to the solution of histidine (15 mL, 0.1 M), then stored at 25 C for 2 h without light. The concentration of the as-synthesized His-Au NCs was 2500 mM.…”

“…23 Most of the currently available detection methods were based on instrumental analysis. [24][25][26][27] For example, Mare et al 24 achieved MES detection by using chromatography combined with uorescence detection; Rizk et al 25 adopted high performance liquid chromatography (HPLC) method for simultaneous separation and quantication of MES in the presence of its degradation products; Salman et al 26 showed a reliable and specic LC-MS/MS method for detection of MES and Dimesna; Ling et al 27 performed capillary liquid chromatography to detect MES. Even though the sensitivity of these instrumental analytical methods is relatively high, they are usually complicated in sample pretreatment and high in operation and instrument cost.…”