New investigations on the guaiacol peroxidase of Opuntia ficus indica L. and its modulation by ascorbic acid and copper. Towards an optimization of quantitative and qualitative tests

Kohaich, K.; Baaziz, M.

doi:10.1016/j.molcatb.2015.05.013

Cited by 4 publications

(2 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, Zdravković-Korać et al [ 61 ] observed a reduction in the activity of POX (extraction with liquid nitrogen and pyrogallol as a substrate) during the transition of friable calluses to ECs of Aesculus flava . A similar decrease in POX activity (ascorbate peroxidase) was observed during the induction of regeneration in Avena nuda [ 62 ]. In addition to the plant species factor, these differences could be related to the POX type, preparation protocol, and the substrates used in activity tests.…”

Section: Discussionsupporting

confidence: 57%

Peroxidase Enzyme Fractions as Markers of Somatic Embryogenesis Capacities in Olive (Olea europaea L.)

Oulbi

Kohaich

Baaziz

et al. 2021

Plants

View full text Add to dashboard Cite

As part of the search for biochemical markers of somatic embryogenesis in tissue cultures of olive (Olea europaea L.), peroxidases (POXs) in both the soluble and ionically wall-bound fractions were studied in two reputed olive cultivars (cvs.): “Picholine Marocaine” and “Dahbia”. In order to carry out embryogenesis induction, proximal cotyledons were cultured in modified olive medium (OMc) supplemented with 25 μM indole-3-butylic acid (IBA) and 2.5 μM 2-isopentenyladenine (2iP), while distal leaf fragments (somatic explants) were cultured in OMc supplemented with 4.56 µM zeatin riboside (ZR) and 10.25 µM 1-naphthaleneacetic acid (NAA). Regarding embryogenic potentials, the zygotic explants (cv. Picholine Marocaine: 43.39%; cv. Dahbia: 53.41%) were more regenerative than the somatic explants (cv. Picholine Marocaine: 13.05%; cv. Dahbia: 19.51%). The enzyme assay showed a higher POX activity in embryogenic calluses (ECs) than in nonembryogenic calluses (NECs) for the zygotic explants in both studied cultivars. When expressed as units per milligram of proteins (U mg−1 proteins), the highest total POXs activities (soluble POXs + ionically wall-bound POXs) were found in the ECs derived from the zygotic explants; for cv. Dahbia, 65% of the enzyme activities came from the ionically wall-bound fractions. Polyacrylamide gel electrophoresis showed that the ECs of the highly active cv. Dahbia were characterized by highly active isoperoxidases that were revealed in four migration zones, particularly a doublet in the A4 zone (Rf 0.70–0.73) present in the ionically wall-bound POXs. The fast-moving anodic POXs of the ionically wall-bound fractions could be adopted as an early electrophoretic test to determine the embryogenesis capacities in olive tissue culture materials. As biochemical markers, the POX enzyme and its profile in fractions, i.e., as soluble POXs and ionically wall-bound POXs, can offer a valuable tool for improving the tissue culture of olive via somatic embryogenesis.

show abstract

Section: Discussionsupporting

confidence: 57%

Peroxidase Enzyme Fractions as Markers of Somatic Embryogenesis Capacities in Olive (Olea europaea L.)

Oulbi

Kohaich

Baaziz

et al. 2021

Plants

View full text Add to dashboard Cite

show abstract

“…p -coumaric and sinapic acids) did not present such behavior (data not shown). In fact, some authors had found specific guaiacol peroxidades from different parts of O. ficus indica species [42].…”

Section: Resultsmentioning

confidence: 99%

Waste residues from Opuntia ficus indica for peroxidase-mediated preparation of phenolic dimeric compounds

Sánchez-Carvajal

Alatorre‐Santamaría²,

Valerio‐Alfaro

et al. 2018

Biotechnology Reports

View full text Add to dashboard Cite

show abstract

Quantitative and Qualitative Aspects of Soluble and Ionically Wall-Bound Peroxidases of EMS-Mutagenized Durum Wheat Lines (Triticum durum), as Tested for Yield at Drought Conditions

et al. 2018

View full text Add to dashboard Cite

New investigations on the guaiacol peroxidase of Opuntia ficus indica L. and its modulation by ascorbic acid and copper. Towards an optimization of quantitative and qualitative tests

Cited by 4 publications

References 27 publications

Peroxidase Enzyme Fractions as Markers of Somatic Embryogenesis Capacities in Olive (Olea europaea L.)

Peroxidase Enzyme Fractions as Markers of Somatic Embryogenesis Capacities in Olive (Olea europaea L.)

Waste residues from Opuntia ficus indica for peroxidase-mediated preparation of phenolic dimeric compounds

Quantitative and Qualitative Aspects of Soluble and Ionically Wall-Bound Peroxidases of EMS-Mutagenized Durum Wheat Lines (Triticum durum), as Tested for Yield at Drought Conditions

Contact Info

Product

Resources

About