New ionic targets of 3,3′,5′-triiodothyronine at the plasma membrane of rat Sertoli cells

Zanatta, Ana Paula; Gonçalves, Renata; Zanatta, Leïla; Oliveria, Geisel Túlio de; Moraes, Ana Luiza Ludwig; Zamoner, Ariane; Fernández-Dueñas, Víctor; Lanznaster, Débora; Ciruela, Francisco; Tasca, Carla I.; Delalande, Christelle; Menegaz, Danusa; Silva, Fátima Regina Mena Barreto

doi:10.1016/j.bbamem.2019.01.002

Cited by 8 publications

(3 citation statements)

References 68 publications

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“…Similarly, 3,3',5,5'-tetraiodothyroacetic acid (TETRAC), derived from deamination of T4, is a powerful ligand of αvβ3 receptor, acting as an efficient competitor for T4/T3 binding, thus inhibiting their effects in a variety of cellular models of tumorigenesis [20,327]. Even rT3 has been reported to induce non-genomic responses in Sertoli cells [328]. Finally, THs can also undergo decarboxylation, giving rise to thyronamines, such as 3-iodothyronamine (T1AM).…”

Section: Discussionmentioning

confidence: 99%

Genomic and Non-Genomic Mechanisms of Action of Thyroid Hormones and Their Catabolite 3,5-Diiodo-L-Thyronine in Mammals

Giammanco

Schiera

Liegro

2020

IJMS

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Since the realization that the cellular homologs of a gene found in the retrovirus that contributes to erythroblastosis in birds (v-erbA), i.e. the proto-oncogene c-erbA encodes the nuclear receptors for thyroid hormones (THs), most of the interest for THs focalized on their ability to control gene transcription. It was found, indeed, that, by regulating gene expression in many tissues, these hormones could mediate critical events both in development and in adult organisms. Among their effects, much attention was given to their ability to increase energy expenditure, and they were early proposed as anti-obesity drugs. However, their clinical use has been strongly challenged by the concomitant onset of toxic effects, especially on the heart. Notably, it has been clearly demonstrated that, besides their direct action on transcription (genomic effects), THs also have non-genomic effects, mediated by cell membrane and/or mitochondrial binding sites, and sometimes triggered by their endogenous catabolites. Among these latter molecules, 3,5-diiodo-L-thyronine (3,5-T2) has been attracting increasing interest because some of its metabolic effects are similar to those induced by T3, but it seems to be safer. The main target of 3,5-T2 appears to be the mitochondria, and it has been hypothesized that, by acting mainly on mitochondrial function and oxidative stress, 3,5-T2 might prevent and revert tissue damages and hepatic steatosis induced by a hyper-lipid diet, while concomitantly reducing the circulating levels of low density lipoproteins (LDL) and triglycerides. Besides a summary concerning general metabolism of THs, as well as their genomic and non-genomic effects, herein we will discuss resistance to THs and the possible mechanisms of action of 3,5-T2, also in relation to its possible clinical use as a drug.

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Section: Discussionmentioning

confidence: 99%

Genomic and Non-Genomic Mechanisms of Action of Thyroid Hormones and Their Catabolite 3,5-Diiodo-L-Thyronine in Mammals

Giammanco

Schiera

Liegro

2020

IJMS

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“…Sertoli cells present a plasma membrane microenvironment that contains, among the different classes of lipids, proteins that function as ionic channels [1,2]. The kinetics of the ionic channels of Sertoli cells coordinate rapid response events, such as the control of plasma membrane potential, by influencing Na + -K + -ATPase [3][4][5][6], the activities of enzymes that are located at the plasma membrane [4], cell secretion as well as genomic responses as nuclear transcription [7][8][9]. Accordingly, the rapid response activities of the Na + -K + -ATPase pump, calcium-dependent potassium channels (Ca 2+ -K + ), ATP-dependent potassium channels (ATP-K + ), and voltage-sensitive potassium currents (Kv) support the function of Sertoli cells to maintain spermatogenesis and fertility.…”

Section: Introductionmentioning

confidence: 99%

Predictions from a mathematical approach to model ionic signaling for rapid responses of Sertoli cells exhibit similarities to pharmacological approaches

Taques,

Gamba,

Menegaz

et al. 2023

Biomed. Phys. Eng. Express

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Sertoli cells are essential for the male reproduction system as they provide morphological support and nutrients for germ cells to guarantee ongoing spermatogenesis. The aim of this work was to predict the electrical properties at the plasma membrane that trigger Sertoli cell rapid responses by involving ionic channels.The rapid responses of Sertoli cells in culture were monitored using patch clamp electrical measurement and compared to data obtained using pharmacological tools (from intact seminiferous tubules). A mathematical model was used to define the roles of potassium channels and the ATP-dependent Na+/K+ pump in these responses. Mathematical data verification was also performed to determine the resting and hormonal stimulated membrane potentials of Sertoli cells in the intact seminiferous tubules and of Sertoli cells in culture (patch clamp measurements).The prediction of these data based on mathematical modeling demonstrated, for the first time, the involvement of potassium channels and the activation of Na+/K+ pump in the hyperpolarization of Sertoli cells and their consequent rapid responses. Moreover, the mathematical analysis showing the involvement of ionic balance in the rapid responses of these cells to hormones, such as follicle-stimulating hormone, is consistent with previous reports obtained using pharmacological techniques in Sertoli cells.Thus, the validation of such data is reliable and represents a first step in the proposition for a mathematical model to predict rapid responses of Sertoli cells to hormonal stimuli.

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“…Sertoli cells present a plasma membrane microenvironment that contains, among the different classes of lipids, proteins that function as ionic channels 1,2 . The kinetics of the ionic channels of Sertoli cells coordinate events, such as the control of plasma membrane potential, by influencing Na + -K + -ATPase [3][4][5][6] , the activities of enzymes that are located at the plasma membrane 4 , cell secretion and nuclear transcription [7][8][9] . Accordingly, the activities of the Na + -K + -ATPase pump, calcium-dependent potassium channels (Ca 2+ -K + ), ATP-dependent potassium channels (ATP-K + ), and voltage-sensitive potassium currents (Kv) support the function of Sertoli cells to maintain spermatogenesis and fertility.…”

Section: Introductionmentioning

confidence: 99%

Predictions from a mathematical approach to model ionic signaling for rapid responses of Sertoli cells exhibit similarities to pharmacological approaches

Taques

Gamba

Menegaz

et al. 2022

Preprint

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Sertoli cells are essential for the male reproduction system as they provide morphological support and nutrients for germ cells to guarantee ongoing spermatogenesis. The aim of this work was to predict the electrical properties at the plasma membrane that trigger Sertoli cell rapid responses. The responses of Sertoli cells in culture to hormone stimulation were monitored using patch clamp electrical measurement and compared to data obtained using pharmacological tools. A mathematical model was used to define the roles of potassium channels and the ATP-dependent Na+/K + pump in these responses. Mathematical data verification was also performed to determine the resting and hormonal stimulated membrane potentials of Sertoli cells collected from intact seminiferous tubules and of Sertoli cells in culture. The prediction of these data based on mathematical modeling demonstrated, for the first time, the involvement of potassium channels and the activation of Na+/K + pump in the hyperpolarization of Sertoli cells and their consequent rapid responses. Moreover, the mathematical analysis showing the involvement of ionic balance in the rapid responses of these cells to hormones, such as follicle-stimulating hormone, is consistent with previous reports obtained using pharmacological techniques in Sertoli cells. Thus, the validation of such data is reliable and represents a first step in the proposition for a mathematical model to predict rapid responses of non-excitable cells to hormonal stimuli.

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New ionic targets of 3,3′,5′-triiodothyronine at the plasma membrane of rat Sertoli cells

Cited by 8 publications

References 68 publications

Genomic and Non-Genomic Mechanisms of Action of Thyroid Hormones and Their Catabolite 3,5-Diiodo-L-Thyronine in Mammals

Genomic and Non-Genomic Mechanisms of Action of Thyroid Hormones and Their Catabolite 3,5-Diiodo-L-Thyronine in Mammals

Predictions from a mathematical approach to model ionic signaling for rapid responses of Sertoli cells exhibit similarities to pharmacological approaches

Predictions from a mathematical approach to model ionic signaling for rapid responses of Sertoli cells exhibit similarities to pharmacological approaches

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