New iron export pathways acting via holo-ferritin secretion

Yanatori, Izumi; Kishi, Fumio; Toyokuni, Shinya

doi:10.1016/j.abb.2023.109737

Cited by 7 publications

(2 citation statements)

References 58 publications

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“…Sitokin-sitokin tersebut menginduksi apoptosis pada erythroid burst-forming unit dan colony-forming unit. Aksi tersebut sebagian diperantarai oleh aksi ceramide serta penurunan jumlah reseptor eritropoietin di dalam prekursor sel eritroid (Yanatori et al, 2023). Kehadiran Tabel 3.…”

Section: Hasil Dan Pembahasanunclassified

Profil Hematologi, Kadar Besi (Fe), dan Rasio Mieloid: Eritroid Kuda Hiperimun yang digunakan dalam Produksi Plasma Antisera

Arifianto,

Esfandiari,

Wibawan

et al. 2024

Jurnal Sain Veteriner

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Kuda merupakan salah satu hewan yang penting dalam produksi plasma antisera sebagai bahan baku pembuatan serum. Akhir abad ke-19 kuda berperan krusial dalam pengembangan serum pertama terhadap penyakit difteri manusia. Hingga saat ini kuda masih digunakan untuk memproduksi berbagai serum anti-venom, anti-rabies, anti-tetanus, hingga anti-SARS CoV-2. Proses produksi plasma antisera diawali dengan injeksi imunogen pada kuda yang disebut dengan hiperimunisasi. Antibodi dalam jumlah besar yang terbentuk dikoleksi kemudian dipurifikasi untuk digunakan pada manusia. Hiperimunisasi menimbulkan berbagai efek samping, diantaranya adalah penimbunan fibril amiloid pada berbagai jaringan yang dapat mengganggu fisiologi dan fungsi organ tubuh. Sebanyak 12 ekor kuda digunakan dalam penelitian ini dan dibagi menjadi 4 kelompok. Kuda kontrol (n=3) terdiri atas kuda yang belum pernah mendapatkan perlakuan hiperimunisasi, sedangkan 9 kuda lainnya dikelompokkan berdasarkan lamanya waktu produksi plasma antisera dengan metode hiperimunisasi (masing-masing kelompok berjumlah 3 ekor). Kelompok I terdiri atas kuda dengan masa produksi 2-3 tahun, kelompok II terdiri atas kuda dengan masa produksi 4-5 tahun, dan kelompok III terdiri atas kuda dengan masa produksi 6-7 tahun. Sampel darah, serum, dan aspirasi sumsum tulang dianalisis utuk melihat profil eritrosit, Hb, PCV, trombosit, profil besi (besi serum, UIBC, TIBC, saturasi transferin), rasio mieloid:eritroid (M:E) dan persentase retikulosit. Analisis statistik menunjukkan bahwa terdapat perbedaan yang signifikan pada parameter eritrosit dan rasio M:E (P<0,05), sedangkan Hb, trombosit, dan profil besi tidak ditemukan perbedaan yang signifikan (P>0,05). Hal tersebut menunjukkan bahwa perlakuan hiperimunisasi menyebabkan terjadinya anemia non-regeneratif dengan mekanisme supresi eritropoiesis sumsum tulang tanpa memengaruhi komponen besi.

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Section: Hasil Dan Pembahasanunclassified

Profil Hematologi, Kadar Besi (Fe), dan Rasio Mieloid: Eritroid Kuda Hiperimun yang digunakan dalam Produksi Plasma Antisera

Arifianto,

Esfandiari,

Wibawan

et al. 2024

Jurnal Sain Veteriner

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“…It is one of the two subunits forming unbound ferritin, which is linked to iron homeostasis and delivery to cells. Ferritin is also known to influence tumor microenvironment and immunity and is involved in ferroptosis, a type of programmed cell death [37]. In contrast, the other protein, chloride intracellular channel protein 1 (CLC1), was downregulated by P2X7R stimulation of GSCs.…”

Section: Influence Of the P2x7r Stimulation In Cultured Gscs On The E...mentioning

confidence: 99%

Changes Induced by P2X7 Receptor Stimulation of Human Glioblastoma Stem Cells in the Proteome of Extracellular Vesicles Isolated from Their Secretome

Di Giuseppe,

Ricci-Vitiani,

Pallini

et al. 2024

Cells

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Extracellular vesicles (EVs) are secreted from many tumors, including glioblastoma multiforme (GBM), the most common and lethal brain tumor in adults, which shows high resistance to current therapies and poor patient prognosis. Given the high relevance of the information provided by cancer cell secretome, we performed a proteomic analysis of microvesicles (MVs) and exosomes (EXOs) released from GBM-derived stem cells (GSCs). The latter, obtained from the brain of GBM patients, expressed P2X7 receptors (P2X7Rs), which positively correlate with GBM growth and invasiveness. P2X7R stimulation of GSCs caused significant changes in the EV content, mostly ex novo inducing or upregulating the expression of proteins related to cytoskeleton reorganization, cell motility/spreading, energy supply, protection against oxidative stress, chromatin remodeling, and transcriptional regulation. Most of the induced/upregulated proteins have already been identified as GBM diagnostic/prognostic factors, while others have only been reported in peripheral tumors. Our findings indicate that P2X7R stimulation enhances the transport and, therefore, possible intercellular exchange of GBM aggressiveness-increasing proteins by GSC-derived EVs. Thus, P2X7Rs could be considered a new druggable target of human GBM, although these data need to be confirmed in larger experimental sets.

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Challenges in Exploiting Human H Ferritin Nanoparticles for Drug Delivery: Navigating Physiological Constraints

Macone,

Cappelletti,

Incocciati

et al. 2024

WIREs Nanomed Nanobiotechnol

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Over the past two decades, ferritin has emerged as a promising nanoparticle for drug delivery, catalyzing the development of numerous prototypes capable of encapsulating a wide array of therapeutic agents. These ferritin‐based nanoparticles exhibit selectivity for various molecular targets and are distinguished by their potential biocompatibility, unique symmetrical structure, and highly controlled size. The hollow interior of ferritin nanoparticles allows for efficient encapsulation of diverse therapeutic agents, enhancing their delivery and effectiveness. Despite these promising features, the anticipated clinical advancements have yet to be fully realized. As a physiological protein with a central role in both health and disease, ferritin can exert unexpected effects on physiology when employed as a drug delivery system. Many studies have not thoroughly evaluated the pharmacokinetic properties of the ferritin protein shell when administered in vivo, overlooking crucial aspects such as biodistribution, clearance, cellular trafficking, and immune response. Addressing these challenges is crucial for achieving the desired transition from bench to bedside. Biodistribution studies need to account for ferritin's natural accumulation in specific organs (liver, spleen, and kidneys), which may lead to off‐target effects. Moreover, the mechanisms of clearance and cellular trafficking must be elucidated to optimize the delivery and reduce potential toxicity of ferritin nanoparticles. Additionally, understanding the immune response elicited by exogenous ferritin is essential to mitigate adverse reactions and enhance therapeutic efficacy. A comprehensive understanding of these physiological constraints, along with innovative solutions, is essential to fully realize the therapeutic potential of ferritin nanoparticles paving the way for their successful clinical translation.

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New iron export pathways acting via holo-ferritin secretion

Cited by 7 publications

References 58 publications

Profil Hematologi, Kadar Besi (Fe), dan Rasio Mieloid: Eritroid Kuda Hiperimun yang digunakan dalam Produksi Plasma Antisera

Profil Hematologi, Kadar Besi (Fe), dan Rasio Mieloid: Eritroid Kuda Hiperimun yang digunakan dalam Produksi Plasma Antisera

Changes Induced by P2X7 Receptor Stimulation of Human Glioblastoma Stem Cells in the Proteome of Extracellular Vesicles Isolated from Their Secretome

Challenges in Exploiting Human H Ferritin Nanoparticles for Drug Delivery: Navigating Physiological Constraints

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