New Isocoumarins from an Endophytic Fungal Strain Diaporthe arengae M2 and their Antibacterial Activities

Abstract

Four new isocoumarin derivatives 12‐O‐acetyl‐isocitreoisocoumarinol (1), (+)‐(10R)‐O‐acetyl‐diaportinol (2‐a), (‐)‐(10S)‐O‐acetyl‐diaportinol (2‐b), peyroisocoumarin E (3) and new stereoconfigurations of three isocoumarin derivatives desmethyldichlorodiaportinol A (4), threo‐monochlorodiaportinol A (5‐a), erytheo‐monochlorodiaportinol A (5‐b), together with nine known ones (6‐14), were separated from the rice fermentation of endophytic fungus Diaporthe arengae M2 isolated from Camellia oleifera. The structures of new compounds were determined by extensive spectroscopic analyses including nuclear magnetic resonance (NMR) and high resolution electrospray ionization mass spectroscopy (HR‐ESI‐MS). Compounds 4, 7, 8, 12, 13 exhibited definite inhibition against five strains of bacteria with the MIC values range from 16 μg/mL to 64 μg/mL.