New lumbar method for monitoring cerebrospinal fluid pressure in rats

Kusaka, Gen; Calvert, John W.; Smelley, Christopher; Nanda, Anil; Zhang, Junyi

doi:10.1016/j.jneumeth.2003.12.013

Cited by 25 publications

(20 citation statements)

References 23 publications

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“…Second, it is cheaper, more accessible and requires less complex logistics for experiments, allowing for a decrease in cost and increase in sample size of preliminary studies. Third, the basal values of CSF pressure that we found in the rat (in horizontal position) as well as those reported in the literature range from 4 to 7 mmHg [40,41,48] which is close to the values reported in cats [20], pigs [46] and humans [6].…”

Section: Discussionsupporting

confidence: 90%

“…This emphasises the need for measuring CSF pressure caudal and rostral to the lesion. In our study, we only measured the CSF pressure caudal to the lesion but in rat, it is also possible to measure CSF pressure rostral to the injury level: Barth et al [40] have proposed a technique to measure CSF pressure in the cisterna magna by inserting a catheter through the atlantooccipital membrane, whereas Kusaka et al [48] have simultaneously measured intracranial and spinal CSF pressure. In our study, the Th10 level of the experimental SCI does not preclude the surgical approach of the atlantooccipital membrane and the relevance of the present model should be strongly improved by including the measurement of the CSF pressure rostral to the lesion.…”

Section: Discussionmentioning

confidence: 99%

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Rat model of spinal cord injury preserving dura mater integrity and allowing measurements of cerebrospinal fluid pressure and spinal cord blood flow

et al. 2013

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Purposes Cerebrospinal fluid (CSF) pressure elevation may worsen spinal cord ischaemia after spinal cord injury (SCI). We developed a rat model to investigate relationships between CSF pressure and spinal cord blood flow (SCBF). Methods Male Wistar rats had SCI induced at Th10 (n = 7) or a sham operation (n = 10). SCBF was measured using laser-Doppler and CSF pressure via a sacral catheter. Dural integrity was assessed using subdural methyleneblue injection (n = 5) and myelography (n = 5). Results The SCI group had significantly lower SCBF (p \ 0.0001) and higher CSF pressure (p \ 0.0001) values compared to the sham-operated group. Sixty minutes after SCI or sham operation, CSF pressure was 8.6 ± 0.4 mmHg in the SCI group versus 5.5 ± 0.5 mmHg in the shamoperated group. No dural tears were found after SCI. Conclusion Our rat model allows SCBF and CSF pressure measurements after induced SCI. After SCI, CSF pressure significantly increases.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Rat model of spinal cord injury preserving dura mater integrity and allowing measurements of cerebrospinal fluid pressure and spinal cord blood flow

et al. 2013

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“…A telemetric physiologic monitoring system with implantable pressure transmitters (PA-C10 and C-50PXT Data Sciences International, St Paul, USA) was used for blood, CSF, or brain parenchyma pressure measurement. Catheters (0.4 mm outer diameter) were inserted into the subarachnoid space at the fifth lumbar vertebra (Kusaka et al 2004), the cisterna magna after partial laminectomy of first cervical vertebra, or the left frontal cerebrum through a 0.5 mm hole in the skull, and anchored with a ligature and cyanoacrylate glue (Vet Bond, 3M Health Care, St Paul, MN). The transmitter was placed in a skin pouch over the lumbar area or scapular area.…”

Section: Animal Preparationmentioning

confidence: 99%

Simultaneous determination of mechanical properties and physiologic parameters in living rat brain

Shulyakov

Fernando

Cenkowski

et al. 2008

Biomech Model Mechanobiol

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Mechanical properties were determined in living adult rat brain. Reconstructed magnetic resonance images of the rat brain before and after 2 mm compression with a 4.06 mm diameter vinyl screw showed the total volumetric strain was maximal at the site of indentation. The pressure response to stepwise brain compression showed a linear relationship to the point of respiratory compromise. Instrumented indentation was performed on live brain with intact dura using a 4-mm-diameter flat punch indenter to a maximum depth 1.2 mm at loading-unloading rates not exceeding 0.34 N/min and 1.2 mm/min. The calculated elastic modulus showed no consistent change after death. Creep deformation over 15 s was 7.86+/-1.6% in live brain and 27.0+/-0.24% after death. In constant multicycle indentation, displacement from 1st to 10th cycle increased 8.0+/-1.7% in life and 12.9+/-2.8% after death. The results suggest that elastic properties of rat intracranial contents do not change immediately after death, while changes in the viscous properties are substantial. The process of measuring these properties can alter physiological parameters.

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“…In this animal model, we have observed a marked effect of PP1 on brain edema as shown in Figure 2. Neurological scoring is routinely employed in animal models studying neurological disorders, such as middle cerebral artery occlusion model for ischemic stroke [14], endovascular perforation model for subarachnoid hemorrhage [25], and spinal cord injury models [3]. However, these scoring systems were inappropriate for the present model as the affected brain area is localized and the type of injury is different.…”

Section: Brain Injuries Produced By This Animal Modelmentioning

confidence: 99%

Neuroprotection against surgically induced brain injury

Jadhav

Solaroğlu

Obenaus³

et al. 2007

Surgical Neurology

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Background-Neurosurgical procedures are carried out routinely in health institutions across the world. A key issue to be considered during neurosurgical interventions is that there is always an element of inevitable brain injury that results from the procedure itself due to the unique nature of the nervous system. Brain tissue at the periphery of the operative site is at risk of injury by various means including incisions and direct trauma, electrocautery, hemorrhage, and retractor stretch.

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New lumbar method for monitoring cerebrospinal fluid pressure in rats

Cited by 25 publications

References 23 publications

Rat model of spinal cord injury preserving dura mater integrity and allowing measurements of cerebrospinal fluid pressure and spinal cord blood flow

Rat model of spinal cord injury preserving dura mater integrity and allowing measurements of cerebrospinal fluid pressure and spinal cord blood flow

Simultaneous determination of mechanical properties and physiologic parameters in living rat brain

Neuroprotection against surgically induced brain injury

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