New luminometric method for quantification of biological sulfur nucleophiles with the participation of 9‐cyano‐10‐methylacridinium salt

Abstract

Biologically active compounds containing sulfhydryl groups (RSHs: N-acetyl-Lcysteine, D-penicillamine, glutathione and acetylthiocholine chloride) were used to develop a luminometric method for their quantification. The title substrate capable of chemiluminescence (CL) was isolated in a highly pure state as a chloride salt (99.9% using RP-HPLC) and identified using mass spectrometry (ESI Q-TOF) and 1 H NMR spectroscopy. The cation included in the salt, 9-CMA + , underwent oxidation in an alkaline environment containing RSHs by molecular oxygen, generating CL of various intensities, with no need for the use of hydrogen peroxide. The amount of produced light was linearly proportional to the content of investigated analytes in the system over the concentration range $0.2-2 μM, with the detection limits in the range 0.19-1.73 μM. The mechanism of chemiluminogenic oxidation of 9-CMA + in the

Development of a Coelenterazine Derivative with Enhanced Superoxide Anion-Triggered Chemiluminescence in Aqueous Solution

“…In fact, both CL and BL have been gaining practical applications in fields as different as real-time imaging [9][10][11] and cancer therapy [12][13][14]. Nevertheless, the most widespread use of CL and BL substrates/reactions is arguably in sensing applications [15][16][17][18][19][20].…”

Preparation of a novel group of chemiluminescent N‐substituted acridinium esters

“…Consequently, many AEs have been synthesized and their applications have been investigated. [ 3–18 ]…”

“…Consequently, many AEs have been synthesized and their applications have been investigated. [3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18] The AE label 1 (Figure 1), containing a succinimidyl ester [Nhydroxysuccinimide (NHS) ester], was developed in 1983. [19] Much effort has been made to modify the structure of 1 to improve its performance.…”

Comparative Investigation of the Chemiluminescent Properties of a Dibrominated Coelenterazine Analog

“…The fact that these systems are independent of an external light source for activation also eliminates the problem of light penetration into biologic tissues, except for the resulting light emission. Besides its applications in real-time imaging [ 6 , 8 , 9 ], these systems show anticancer activity [ 10 , 11 , 12 ] but are mostly used in sensing applications [ 13 , 14 , 15 ].…”