New Macrolide-Lincosamide-Streptogramin B Resistance Gene
            <i>erm</i>
            (48) on the Novel Plasmid pJW2311 in Staphylococcus xylosus

Wipf, Juliette Ramona Karin; Riley, Matthew C.; Kania, Stephen A.; Bemis, David A.; Andreis, Sabrina Nathalie; Schwendener, Sybille; Perreten, Vincent

doi:10.1128/aac.00066-17

Cited by 19 publications

(7 citation statements)

References 23 publications

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“…Colonization of farm animals by these pathogens, specifically non-typhoidal Salmonella (NTS) serovars, presents a significant food safety dilemma for the poultry industry. , More importantly, multi-AMR pathogenic bacterial strains have been isolated from both humans and animals making the problem more complicated and deeply worrisome. The rise of antibiotic resistance in bacteria (such as the β-lactam-resistant Gram-negative Enterobacteriaceae and the macrolide–lincosamide–streptogramin B in Gram-positive bacteria) is of particular concern. − If nothing is done, it has been estimated that AMR could cause more deaths than cancer with a pinnacle global economic damage, similar to that of the 2008 financial crisis, by 2050. , As a result of the aforementioned reasons, the use of cost-effective strategies in the production of antibiotic-free animals with improved health and productivity will provide the industry with alternative solutions while increasing the public trust in antibiotic use policies as well as decreasing problems associated with AMR pathogens. Despite having many regulatory agencies recognizing the importance of withdrawing and banning antibiotics from use other than clinical applications, , the narrow profitability margins in addition to the high costs of production within the animal/feed industries are among the top reasons for producers to be reluctant of embracing a complete antibiotic ban in animal feed …”

Section: Amr In Agriculturementioning

confidence: 99%

Grape Pomace as a Promising Antimicrobial Alternative in Feed: A Critical Review

Hassan

Kosir

et al. 2019

J. Agric. Food Chem.

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Antimicrobial resistance is among the most urgent global challenges facing sustainable animal production systems. The use of antibiotics as growth promoters and for infectious disease prevention in intensive animal-farming practices has translated into the selection and spread of antimicrobial resistance genes in an unprecedented fashion. Several multiresistant bacterial strains have been isolated from food-producing animals, thus constituting an alarming food-safety issue. Many industrial byproducts with potential antimicrobial properties are currently being investigated to identify empirical and affordable solutions/alternatives that can potentially be used in feed for animals. Grape pomace is among such byproducts that gained the attention as a result of its low cost, abundance, and, most importantly, its bioactive and antibacterial properties. This review discusses the recently reported studies with regard to exploring the use of grape pomace (and its extracts) in animal production to control pathogens, along with the promotion of beneficial bacterial species in the gut to ultimately alleviate antibacterial resistance. The review further summarizes realistic expectations connected with grape pomace usage and lists the still-to-beaddressed concerns about its application in animal agriculture.

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Section: Amr In Agriculturementioning

confidence: 99%

Grape Pomace as a Promising Antimicrobial Alternative in Feed: A Critical Review

Hassan

Kosir

et al. 2019

J. Agric. Food Chem.

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“…Truncation of a variety of helices and loops of domain V led to the identification of smaller RNA structures methylated by Erm proteins, ranging from as small as 27-nucleotide (nt) RNA ( 9 ), 24-nt RNA ( 10 ), and 32-nt RNA ( 11 ) to 41-nt RNA ( 12 ). Almost 40 different erm methyltransferase genes have been isolated and characterized from diverse sources, ranging from pathogens to antibiotic-producing actinomycetes ( 1 , 2 , 13 , 14 ). Despite the diversity of sources, the significant homology among Erm proteins and their common functional role in methylating the specific adenine residue at position N 6 suggest that they could assume similar structures ( 11 , 15 ) and originate from a common ancestor ( 2 , 16 ).…”

Section: Introductionmentioning

confidence: 99%

Plausible Minimal Substrate for Erm Protein

Lee

Park

Jin

2020

Antimicrob Agents Chemother

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Erm proteins methylate a specific adenine residue (A2058, Escherichia coli coordinates) conferring MLSB (macrolide–lincosamide–streptogramin B) antibiotic resistance on a variety of microorganisms ranging from antibiotic producers to pathogens. To identify the minimal motif required to be recognized and methylated by Erm protein, various RNA substrates from 23S rRNA were constructed, and the substrate activity of these constructs was studied using three Erm proteins: ErmB from Firmicutes and ErmE and ErmS from Actinobacteria. The shortest motif of 15 nt could be recognized and methylated by ErmS, consisting of A2051 to the methylatable adenine (A2058) and its base-pairing counterpart strand presumably assuming a quite similar structure to that in 23S rRNA, an unpaired target adenine immediately followed by an irregular double-stranded RNA region. This observation confirms the ultimate end of each side in stem 73 for methylation, determined by the approaches described above, and could reveal the mechanism behind the binding, recognition, induced fit, methylation, and conformational change for product release in the minimal context of substrate, presumably with the help of structural determination of protein–RNA complex. In the course of determining the minimal portion of substrate from domain V, protein-specific features could be observed among the Erm proteins in terms of the methylation of RNA substrate and cooperativity and/or allostery between the region in stem 73 furthest away from the target adenine and the large portion of domain V above the methylatable adenine.

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“…Whereas recent reports illustrate that NAS may harbor resistance determinants (Srednik et al, 2017b ; Wipf et al, 2017 ) including genes considered potential public health hazards (Srednik et al, 2017a ), the prevalence of AMR and respective resistance determinants in NAS isolated from dairy herds remains unknown. It is noteworthy that the vast majority of studies designed to estimate the prevalence of AMR in NAS isolated from dairy herds are regional and limit screening of resistance determinants to resistant isolates, usually by PCR or a similar approach.…”

Section: Introductionmentioning

confidence: 99%

Prevalence and Genetic Basis of Antimicrobial Resistance in Non-aureus Staphylococci Isolated from Canadian Dairy Herds

et al. 2018

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Emergence and spread of antimicrobial resistance is a major concern for the dairy industry worldwide. Objectives were to determine: (1) phenotypic and genotypic prevalence of drug-specific resistance for 25 species of non-aureus staphylococci, and (2) associations between presence of resistance determinants and antimicrobial resistance. Broth micro-dilution was used to determine resistance profiles for 1,702 isolates from 89 dairy herds. Additionally, 405 isolates were sequenced to screen for resistance determinants. Antimicrobial resistance was clearly species-dependent. Resistance to quinupristin/dalfopristin was common in Staphylococcus gallinarum (prevalence of 98%), whereas S. cohnii and S. arlettae were frequently resistant to erythromycin (prevalence of 63 and 100%, respectively). Prevalence of resistance was 10% against β-lactams and tetracyclines. In contrast, resistance to antimicrobials critically important for human medicine, namely vancomycin, fluoroquinolones, linezolid and daptomycin, was uncommon (< 1%). Genes encoding multidrug-resistance efflux pumps and resistance-associated residues in deducted amino acid sequences of the folP gene were the most frequent mechanisms of resistance, regardless of species. The estimated prevalence of the mecA gene was 17% for S. epidermidis. Several genes, including blaZ, mecA, fexA, erm, mphC, msrA, and tet were associated with drug-specific resistance, whereas other elements were not. There were specific residues in gyrB for all isolates of species intrinsically resistant to novobiocin. This study provided consensus protein sequences of key elements previously associated with resistance for 25 species of non-aureus staphylococci from dairy cattle. These results will be important for evaluating effects of interventions in antimicrobial use in Canadian dairy herds.

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New Macrolide-Lincosamide-Streptogramin B Resistance Gene erm (48) on the Novel Plasmid pJW2311 in Staphylococcus xylosus

Cited by 19 publications

References 23 publications

Grape Pomace as a Promising Antimicrobial Alternative in Feed: A Critical Review

Grape Pomace as a Promising Antimicrobial Alternative in Feed: A Critical Review

Plausible Minimal Substrate for Erm Protein

Prevalence and Genetic Basis of Antimicrobial Resistance in Non-aureus Staphylococci Isolated from Canadian Dairy Herds

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