New magnetic-resonance-imaging-visible poly(ε-caprolactone)-based polyester for biomedical applications

Blanquer, Sébastien; Guillaume, Olivier; Letouzey, V.; Lemaire, Laurent; Franconi, Florence; Paniagua, C.; Coudane, Jean; Garric, Xavier

doi:10.1016/j.actbio.2011.11.009

Cited by 26 publications

(28 citation statements)

References 35 publications

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“…It is a cell-membrane-associated enzyme, expressed also in matrix vesicles, and in association with other proteins enhances deposition of hydroxyapatite along the collagen fibrils. ALP hydrolyzes its substrate, inorganic pyrophosphate, to inorganic phosphate, the latter being a substrate for the HA mineral [60]. In our experiments, the highest activity of ALP was found in the samples obtained from the lysate of cells cultivated on Ti SiO 2 implants.…”

Section: Discussionmentioning

confidence: 56%

Comparative in vitro study regarding the biocompatibility of titanium-base composites infiltrated with hydroxyapatite or silicatitanate

et al. 2014

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BackgroundThe development of novel biomaterials able to control cell activities and direct their fate is warranted for engineering functional bone tissues. Adding bioactive materials can improve new bone formation and better osseointegration. Three types of titanium (Ti) implants were tested for in vitro biocompatibility in this comparative study: Ti6Al7Nb implants with 25% total porosity used as controls, implants infiltrated using a sol–gel method with hydroxyapatite (Ti HA) and silicatitanate (Ti SiO2). The behavior of human osteoblasts was observed in terms of adhesion, cell growth and differentiation.ResultsThe two coating methods have provided different morphological and chemical properties (SEM and EDX analysis). Cell attachment in the first hour was slower on the Ti HA scaffolds when compared to Ti SiO2 and porous uncoated Ti implants. The Alamar blue test and the assessment of total protein content uncovered a peak of metabolic activity at day 8–9 with an advantage for Ti SiO2 implants. Osteoblast differentiation and de novo mineralization, evaluated by osteopontin (OP) expression (ELISA and immnocytochemistry), alkaline phosphatase (ALP) activity, calcium deposition (alizarin red), collagen synthesis (SIRCOL test and immnocytochemical staining) and osteocalcin (OC) expression, highlighted the higher osteoconductive ability of Ti HA implants. Higher soluble collagen levels were found for cells cultured in simple osteogenic differentiation medium on control Ti and Ti SiO2 implants. Osteocalcin (OC), a marker of terminal osteoblastic differentiation, was most strongly expressed in osteoblasts cultivated on Ti SiO2 implants.ConclusionsThe behavior of osteoblasts depends on the type of implant and culture conditions. Ti SiO2 scaffolds sustain osteoblast adhesion and promote differentiation with increased collagen and non-collagenic proteins (OP and OC) production. Ti HA implants have a lower ability to induce cell adhesion and proliferation but an increased capacity to induce early mineralization. Addition of growth factors BMP-2 and TGFβ1 in differentiation medium did not improve the mineralization process. Both types of infiltrates have their advantages and limitations, which can be exploited depending on local conditions of bone lesions that have to be repaired. These limitations can also be offset through methods of functionalization with biomolecules involved in osteogenesis.

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Section: Discussionmentioning

confidence: 56%

Comparative in vitro study regarding the biocompatibility of titanium-base composites infiltrated with hydroxyapatite or silicatitanate

et al. 2014

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“…We limited our study to low ratios because our previous work on MRI-visible PCLs proved the efficient visualization of polymers incorporating low amounts of contrast agents. 6 MRI-PCLs 4 were finally obtained by a Cu I -catalyzed [3 + 2] cycloaddition between the azide groups of 2 and the alkyne groups of 3. Typically, a 3 equiv excess of 2 relative to the alkyne moles present in copolymers 3 was used and reaction was performed in a dilute regime to avoid nondesired intermolecular cross-linking and to favor the reaction of 2 with a single propargyl group (Scheme 2).…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

MRI-Visible Poly(ε-caprolactone) with Controlled Contrast Agent Ratios for Enhanced Visualization in Temporary Imaging Applications

Habnouni¹,

Nottelet²,

Darcos³

et al. 2013

Biomacromolecules

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Hydrophobic macromolecular contrast agents (MMCAs) are highly desirable to provide safe and efficient magnetic resonance (MR) visibility to implantable medical devices. In this study, we report on the synthesis and evaluation of novel biodegradable poly(ε-caprolactone)-based MMCAs. Poly(α-propargyl-ε-caprolactone-co-ε-caprolactone)s containing 2, 5, and 10 mol % of propargyl groups have been prepared by ring-opening copolymerization of ε-caprolactone and the corresponding propargylated lactone. In parallel, a diazido derivative of the clinically used diethylenetriaminepentaacetic acid (DTPA)/Gd(3+) complex has been synthesized. Finally, MRI-visible poly(ε-caprolactone)s (PCLs) were obtained by the efficient click ligation of these compounds via a Cu(I)-catalyzed [3 + 2] cycloaddition. ICP-MS analyses confirmed the efficient coupling of the complex on the PCL backbone with the MRI-visible PCLs containing 1.0, 2.6, and 3.6 wt % of Gd(3+). The influence of the Gd(3+) grafting density on the T1 relaxation times and on the MRI visibility of the novel biodegradable MMCAs was evaluated. Finally, their stability and cytocompatibility were assessed with regard to their potential as innovative MRI-visible biomaterials for biomedical applications.

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“…Ces dernières années, quelques équipes de recherche tentent d'incorporer des systèmes au sein de treillis afin de les localiser en IRM, mais à ce jour très peu de résultats sont concluants [24,25]. La stratégie chimique développée au laboratoire a permis de greffer de manière durable un complexant du Gd 3+ (le DTPA), sur le squelette de polymère dégradable (PCL) ou biostable (PAM) pour viser des applications biomédicales temporaires ou permanentes [12,13]. Les polymères obtenus (PCL-DTPA-Gd et PAM-DTPA-Gd) induisent un effet T1 significatif lors de l'analyse en IRM 7 Tesla.…”

Section: Discussionunclassified

“…Une méthode de modification par activation anionique déjà décrite au laboratoire est utilisée afin de greffer de manière covalente et avec une liaison de type carbone-carbone le DTPA (complexant du Gd 3+ ) sur la PCL ou sur le PAM [12,13]. La illustre l'absence de rehaussement de signal du gel seul (1) ou après inclusion de la PCL-DTPA (2).…”

Section: Synthèse Des Polymères-dtpa-gd Et Visualisation En Irmunclassified

“…Parallèlement, des polymères ont été synthétisés par activation anionique permettant de greffer de manière covalente et durable un agent de contraste en IRM (DTPA-Gd) sur, soit un polymère dégradable, la poly(-caprolactone) (PCL), soit un polymère biostable, le poly(acrylate de méthyle) (PAM) [11][12][13]. La présence de cet agent de contraste sur la chaîne des polymères permet de rehausser le signal des poudres de polymères.…”

Section: Introductionunclassified

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Conception d’un treillis anti-infectieux et visible en IRM pour la prise en charge chirurgicale des prolapsus génitaux et des hernies abdominales

Guillaume¹,

Blanquer²,

Letouzey³

et al. 2012

IRBM

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New magnetic-resonance-imaging-visible poly(ε-caprolactone)-based polyester for biomedical applications

Cited by 26 publications

References 35 publications

Comparative in vitro study regarding the biocompatibility of titanium-base composites infiltrated with hydroxyapatite or silicatitanate

Comparative in vitro study regarding the biocompatibility of titanium-base composites infiltrated with hydroxyapatite or silicatitanate

MRI-Visible Poly(ε-caprolactone) with Controlled Contrast Agent Ratios for Enhanced Visualization in Temporary Imaging Applications

Conception d’un treillis anti-infectieux et visible en IRM pour la prise en charge chirurgicale des prolapsus génitaux et des hernies abdominales

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