New Meroterpenoid and Isocoumarins from the Fungus Talaromyces amestolkiae MST1-15 Collected from Coal Area

Abstract

Three new compounds including a meroterpenoid (1) and two isocoumarins (8 and 9), together with thirteen known compounds (2–7, 10–16) were isolated from the metabolites of Talaromyces amestolkiae MST1-15. Their structures were identified by a combination of spectroscopic analysis. The absolute configuration of compound 1 was elucidated on the basis of experimental and electronic circular dichroism calculation, and compounds 8 and 9 were determined by Mo2(OAc)4-induced circular dichroism experiments. Compounds 7–16 showed weak antibacterial activities against Stenotrophomonas maltophilia with MIC values ranging from 128 to 512 μg/mL (MICs of ceftriaxone sodium and levofloxacin were 128 and 0.25 μg/mL, respectively).

Phenylhydrazone Alkaloids from the Deep-Sea Cold Seep Derived Fungus Talaromyces amestolkiae HDN21-0307

“…HPLC and LC-MS chromatograms of the extract from strain HDN21-0307 grown in Fungus 2# liquid media under shaking conditions showed four major peaks with distinctive UV absorptions (λ max ∼ 220, 270, 480 nm/225, 280, 400, 540 nm), and their molecular weights were 351, 564, 310, and 326 Da, respectively (Figure S3). It is interesting to note that none of the previously reported natural products from T. amestolkiae displayed this distinct UV profile. − This result suggests that the Fungus 2# liquid media induced the production of new metabolites in T. amestolkiae .…”

“…The metabolites of T. amestolkiae previously reported by other research groups primarily consist of coumarins and terpenes, predominantly isolated from solid-substrate media (Figure S1). − Hence, an OSMAC strategy using six different liquid media under either static or shaking conditions was employed to further explore the metabolic potential of T. amestolkiae (Figure S2). HPLC and LC-MS chromatograms of the extract from strain HDN21-0307 grown in Fungus 2# liquid media under shaking conditions showed four major peaks with distinctive UV absorptions (λ max ∼ 220, 270, 480 nm/225, 280, 400, 540 nm), and their molecular weights were 351, 564, 310, and 326 Da, respectively (Figure S3).…”

“…Talaromyces , a genus belonging to the family Trichocomaceae, produced a wide range of secondary metabolites, including esters, polyketones, anthraquinones, terpenoids, meroterpenoids, steroids, and alkaloids. , Most of these show significant biological activities, such as antibacterial, antifungal, antitumor, anti-inflammatory, and antioxidant activities. , Talaromyces amestolkiae has remained relatively unexplored for secondary metabolites, and the secondary metabolites that have been reported so far are relatively simple, including mostly coumarins and terpenes (Figure S1). − In our ongoing investigations of bioactive compounds from marine-derived fungi, − T. amestolkiae HDN21-0307, a fungal strain isolated from deep-sea cold seep sediments in the South China Sea, was selected for further research. Employing the one strain–many compounds (OSMAC) strategy for cultivating T. amestolkiae HDN21-0307 led to unexpected findings, as it produced compounds with significantly different UV absorption profiles (series UV absorption around 220, 270, 480 nm/225, 280, 400, 540 nm, Figures S2 and S3), compared to the known compounds of T. amestolkiae . − …”

“… − In our ongoing investigations of bioactive compounds from marine-derived fungi, − T. amestolkiae HDN21-0307, a fungal strain isolated from deep-sea cold seep sediments in the South China Sea, was selected for further research. Employing the one strain–many compounds (OSMAC) strategy for cultivating T. amestolkiae HDN21-0307 led to unexpected findings, as it produced compounds with significantly different UV absorption profiles (series UV absorption around 220, 270, 480 nm/225, 280, 400, 540 nm, Figures S2 and S3), compared to the known compounds of T. amestolkiae . − …”