New method for cryoprotectant-free freezing of human oligoasthenoteratozoospremic spermatozoa with high-molecular polymer

Petrushkо, Maryna; Yurchuk, Taisiia; Todorov, Plamen; Hristova, Elena; Piniaiev, Volodymyr; Isachenko, Evgenia; Rahimi, Gohar; Mallmann, Peter; Isachenko, Vladimir

doi:10.1016/j.cryobiol.2021.09.013

Cited by 8 publications

(4 citation statements)

References 39 publications

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“…16 The inclusion of cryoprotective agents alleviates the cryostorage of living matter by supporting supersaturation (incomplete cell freezing) and enhancing the osmotic strength. 21,22 Unfortunately, their toxicity at high concentrations [23][24][25] imposes the cryopreservation of human spermatozoa in an articial seminal plasma (relying on its self-protective function) without cryoprotectants 26 or via the addition of 5% high-molecular polyvinylpyrrolidone polymer, 27 risking the loss of sperm motility and mitochondrial activity (nearly 40-50%, according to Table S2 † and Fig. 3 in ref.…”

Section: Introductionmentioning

confidence: 99%

Hydrophobic soot nanoparticles as a non-cytotoxic motility activator of human spermatozoa

Esmeryan

Rangelov²,

Chaushev³

2022

Nanoscale Adv.

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Section: Introductionmentioning

confidence: 99%

Hydrophobic soot nanoparticles as a non-cytotoxic motility activator of human spermatozoa

Esmeryan

Rangelov²,

Chaushev³

2022

Nanoscale Adv.

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“…Highly effective methods of low-temperature storage of spermatozoa opened the way to banking and wide clinical application of spermatozoa in ART methods. For all that, the study of the morphological, ultrastructural and functional characteristics of spermatozoa remains relevant [2,3,4].…”

Section: огляди літератури / Literature Reviews Connection Of the Pub...mentioning

confidence: 99%

Does Cryopreservation Uniformly Affect the Dna Fragmentation Rate of Spermatozoa of Men With Different States of Spermatogenesis? A Systematic Review and Meta-Analysis

Petrushko,

Pugovkin,

Shevchenko

et al. 2023

VPBM

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Cryotechnologies have become an integral part of infertility treatment methods. However, the question of the impact of cryopreservation on human sperm DNA fragmentation levels remains open. The aim was to conduct a systematic review and meta-analysis of studies on the effect of the cryopreservation method (a method of rapid cooling and vitrification) on the occurrence of DNA damage in spermatozoa of men with different states of spermatogenesis. The search was carried out in Medline, Embase, PubMed and Google Scholar databases. The results of 52 studies from 32 scientific papers were analysis in the present study. DNA damage in spermatozoa of men with different states of spermatogenesis was measured before and after cryopreservation using one of these methods: SCSA, SCD, TUNEL and Comet Assay. It was found that cryopreservation, regardless of the method used, leads to an increase of 7.73±2.04% in the DNA fragmentation rate of spermatozoa, regardless of the state of spermatogenesis of men. Cryopreservation increases normospermia sperm DNA fragmentation compared to pathospermia (SMD=8.76%, p<0.00001 and SMD=4.01%, p<0.00001, respectively). The greatest effect of cryopreservation on DNA fragmentation compared to fresh sperm was determined by the TUNEL method. Using this method, 8.08±2.38% of cells with the mentioned pathology were identified (SMD=8.08%, 95% CI 5.69 -10.46%, p<0.00001). Determining the degree of DNA damage of spermatozoa before their banking can be a useful marker of the effectiveness of their cryopreservation, as it has prognostic value regarding the effectiveness of infertility treatment by assisted reproductive technology methods.Results. A relationship between high-risk disease and male gender, multifocal lesions, localization in the lower third of the thyroid lobe, superficial location of the tumor, and stromal fibrosis was showing in Sak S. D.`s study. According to the results of studies, size of tumor larger than 1.0 cm and multifocal lesions can be considered an indicator of the aggressive course of PTC. AIT complicates the cytological diagnosis of nodal masses. Cellular atypia, anisonucleosis, and pleomorphism are observed in both AIT and PTC, so differential diagnosis in the case of a combination of these pathologies is more difficult. The basis for this relationship is the molecular, hormonal and histopathological similarity of these diseases. Galectin-3 is a regulator of normal cell proliferation, and its overexpression leads to malignant cell transformation and metastasis. The neoplastic transformation of benign tissue is a complex process influenced by numerous factors. Humoral mechanisms of cancer development are associated with the IgG4 subclass. Regulatory Treg cells are a subgroup of CD4+ cells that suppress the immune response, playing an important role in avoiding the host's antitumor immune response. It is reported that a decrease in CD4+ leads to oncological transformation. The BRAF mutation has also been shown to cause overexpression of many other molecular tumour markers, such...

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“…Maintenance of the morphological and functional features of the gametes, embryo, and accessory cells after cryopreservation and thawing is a key factor affecting the success of ART. Thus, it has been proven that vitrification is a more effective method of preserving oocytes and embryos compared with slow freezing, and a fast two-stage method is more effective for spermatozoa [ 2 , 3 , 4 , 5 ]. Slow freezing is considered a standard method for cryopreservation of tissues and suspensions of somatic cells [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

New Approach to the Cryopreservation of GV Oocytes and Cumulus Cells through the Lens of Preserving the Intercellular Gap Junctions Based on the Bovine Model

Yurchuk,

Likszo,

Witek

et al. 2024

IJMS

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Differences in structural and functional properties between oocytes and cumulus cells (CCs) may cause low vitrification efficiency for cumulus–oocyte complexes (COCs). We have suggested that the disconnection of CCs and oocytes in order to further cryopreservation in various ways will positively affect the viability after thawing, while further co-culture in vitro will contribute to the restoration of lost intercellular gap junctions. This study aimed to determine the optimal method of cryopreservation of the suspension of CCs to mature GV oocytes in vitro and to determine the level of mRNA expression of the genes (GJA1, GJA4; BCL2, BAX) and gene-specific epigenetic marks (DNMT3A) after cryopreservation and in vitro maturation (IVM) in various culture systems. We have shown that the slow freezing of CCs in microstraws preserved the largest number of viable cells with intact DNA compared with the methods of vitrification and slow freezing in microdroplets. Cryopreservation caused the upregulation of the genes Cx37 and Cx43 in the oocytes to restore gap junctions between cells. In conclusion, the presence of CCs in the co-culture system during IVM of oocytes played an important role in the regulation of the expression of the intercellular proteins Cx37 and Cx43, apoptotic changes, and oocyte methylation. Slow freezing in microstraws was considered to be an optimal method for cryopreservation of CCs.

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New method for cryoprotectant-free freezing of human oligoasthenoteratozoospremic spermatozoa with high-molecular polymer

Cited by 8 publications

References 39 publications

Hydrophobic soot nanoparticles as a non-cytotoxic motility activator of human spermatozoa

Hydrophobic soot nanoparticles as a non-cytotoxic motility activator of human spermatozoa

Does Cryopreservation Uniformly Affect the Dna Fragmentation Rate of Spermatozoa of Men With Different States of Spermatogenesis? A Systematic Review and Meta-Analysis

New Approach to the Cryopreservation of GV Oocytes and Cumulus Cells through the Lens of Preserving the Intercellular Gap Junctions Based on the Bovine Model

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